보고서 정보
주관연구기관 |
전북대학교 Chonbuk National University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2013-01 |
과제시작연도 |
2012 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201300014406 |
과제고유번호 |
1395027932 |
사업명 |
지역전략작목산학연협력사업(보조,광특+제주계정) |
DB 구축일자 |
2013-08-26
|
DOI |
https://doi.org/10.23000/TRKO201300014406 |
초록
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III. 연구개발의 내용 및 범위
○ 항생제대체제에 대한 토종닭 장염 예방 효능 및 생산성 평가
○ 장염 예방을 위한 항생제대체제 스크리닝 및 선발
○ 양계산학연협력단 회원 농가 장염 발생 실태조사 및 컨설팅
○ 기존 및 개량된 항생제대체제에 대한 토종닭 장염 예방 및 생산성 평가
○ 기존 항생제대체제 선발 및 개량
○ 장염발생 실태조사 및 항생제대체제 현장적용 효능평가
○ 우수한 항생제대체제 급여에 의한 토종닭 계산물 품질 분석
○ 우수한 항생제대체제 대량생산체계 구축
○ 장염발생
III. 연구개발의 내용 및 범위
○ 항생제대체제에 대한 토종닭 장염 예방 효능 및 생산성 평가
○ 장염 예방을 위한 항생제대체제 스크리닝 및 선발
○ 양계산학연협력단 회원 농가 장염 발생 실태조사 및 컨설팅
○ 기존 및 개량된 항생제대체제에 대한 토종닭 장염 예방 및 생산성 평가
○ 기존 항생제대체제 선발 및 개량
○ 장염발생 실태조사 및 항생제대체제 현장적용 효능평가
○ 우수한 항생제대체제 급여에 의한 토종닭 계산물 품질 분석
○ 우수한 항생제대체제 대량생산체계 구축
○ 장염발생 실태조사 및 항생제대체제 적용프로그램 구축
Abstract
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To evaluate the effects of naturally derived antibiotics the Allium sativum(AS), Atractylodes ovate(AO), Coptidis rhizome(CR), Phellodendron chinense(PC) and Syzygium aromaticum(SA) were selected. All natural antibiotics were mixed to wood vinegar which was collected due to the condensation of vapor
To evaluate the effects of naturally derived antibiotics the Allium sativum(AS), Atractylodes ovate(AO), Coptidis rhizome(CR), Phellodendron chinense(PC) and Syzygium aromaticum(SA) were selected. All natural antibiotics were mixed to wood vinegar which was collected due to the condensation of vapor and smoke under refrigerated temperature, and the mixture was ripened for 50 days under room temperature. All 0.1% natural antibiotic extracts were added to chicken diets, and each diet was supplied to 240 Korean native chicken for 10 weeks. The productivity including body weight, weight gain, feed intake and feed efficiency, and the microorganisms in small intestine were checked on 1, 5 and 10 weeks of growth. The control and other 5 treatments were not significantly differed on Korean native chicken productivity up to 5 weeks of growth, however, Korean native chickens in CR treatment tended to numerically improve in body weight and weight gain. The 0.1% natural antibiotics addition to chicken diet significantly increased the number of total microorganism in Korean native chickens fed SA and CR, in contrast Korean native chickens from PC showed low in total microorganism. The population of lactobacillus was high in Korean native chickens collected from AO, SA and CR treatments but chickens from PC treatment, low in total microorganism, had lower number of lactobacillus in small intestine. The salmonella and E coli were high in CR and SA treatments, and chickens in AO showed a similar number of E coli as compared to that of CR and SA but significantly high population of E coli was determined in AO as compared to that of AS and PC. Therefore, the results indicate that unless no significant differences were determined in Korean native chicken productivity, the Korean native chicken derived from CR may be a natural antibiotic which is able to mix in Korean native chicken diet due to the reaction that was effective in salmonella and E. coli reduction.
To evaluate the antimicrobial and antioxidant effects of ripened medicinal herbsderived by wood vinegar, this study was conducted. The wood vinegar was collected while o만 were heated. The Allium sativum(AS), Atractylodes ovate(AO), Cinnamomum zeylanicum(CZ), Coptidis rhizome(CR), Houttuynia cordata(HC), Phellodendron chinense(PC) and Syzygium aromaticum(SA) extracts were collected using wood vinegar, and they were ripened under room temperature for 50 days. All ripened medicinal herb extracts were used to test the antimicrobial and antioxidant activities. For the lactobacillus, clear zone of 6 different medicinal herb except CR was ranged from 1.28 to 1.63 mm, and 3.30 and 3.48 mm of clear zone were determined when CZ and SA were applied to salmonella and E. Coli, respectively. They showed the largest clear zone as compared to other herbs. The clear zones of CR for salmonella and E. coli were 2.21 and 3.34 mm, and each clear zone of CR was smaller than that of CZ but was similar to that of SA. The amount of polyphenol and flavonoid was the highest in SA and CR, and they were 4.28 and 0.38 mg/mL, respectively. The CR shown 0.38 mg/mL flavonoid, had the highest DPPH and it was 0.41 mM. The DPPH of HZ was significantly lowered in accordance with high amount of polyphenol and flavonoid, 2.56 and 0.20 mg/mL. Therefore, the results indicate that CZ, SA and CR, having the high in antimicrobial and antioxidant effects, may be medicinal herbs used for naturally derived antibiotics in chicken diets.
We evaluated the immunomodulatory effects of a proanthocyanidin-rich extract (PAE) from Pinus radiata bark in Korean native chickens. In experiment 1, proliferation of peripheral blood mononuclear cells (PBMCs) was significantly enhanced in chickens administered 1.25, 2.5, 5, and 10 mg/kg PAE for 2 weeks once daily by oral gavage compared to no administration of PAE in vitro. Proliferation of splenocytes and thymocytes cells was also significantly enhanced in chickens administered 1.25, 2.5, 5, and 10mg/kg PAE for 5 weeks. In experiment 2, proliferation of PBMCs, splenocytes, and thymocytes in chickens administered PAE for 6 weeks was continuously higher than that in other groups. Additionally, increasing cell populations which cell-surface protein expression of CD4+CD8− (Th cells) and Bu-1+ (B cells) was significantly after 4 weeks of PAE administration in chickens. Thus, the immunomodulatory effects of a PAE from P. radiata bark differed by dosing period in chickens.
In this study, systematic analysis of the NE causative pathogens was conducted in Korean native chickens. Causative pathogens of 10 farms (total 61 entity) were analyzed by molecular biologically and histologically. Low frequency of SE (8%), APEC (31%) were detected in liver biopsy but high frequency of CP (92%) were detected in fecal samples. In histological analysis, % of Ei only, Ei/cpb2, cpb2 only samples were estimated as a 10%, 67%, 23%, respectively. Between 23- to 28-day-old flocks (mean 25.6-day-old) NE were judged and 5 to 7 days later in each outbreak farms maximal mortality rate were recorded.
목차 Contents
- 표지 ... 1
- 제출문 ... 2
- 요약문 ... 3
- SUMMARY ... 5
- 목차 ... 8
- 제1장 서 론 ... 9
- 제1절 연구개발의 필요성 ... 9
- 제2장 국내외 기술개발 현황 ... 13
- 제1절 국내 연구 현황 ... 13
- 제2절 국외 연구 현황 ... 16
- 제3장 연구개발수행 내용 및 결과 ... 18
- 제1절 항생제대체제 급여에 따른 토종닭 장염 예방 및 생산성 평가 ... 18
- 제2절 토종닭 장염 예방을 위한 항생제대체제 선발 및 대량생산체계 구축 ... 31
- 제3절 전북지역 토종닭 농가 장염 발생 실태조사 및 항생제대체제 현장 적용프로그램 개발 ... 41
- 제4장 연구개발목표 달성도 및 대외기여도 ... 52
- 제1절 목표대비 대외 달성도 ... 52
- 제2절 정량적 성과 ... 52
- 제5장 연구개발결과의 활용계획 ... 53
- 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 54
- 제7장 기타 중요 변동사항 ... 55
- 제8장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 56
- 제9장 참고문헌 ... 57
- <참고> 주요 연구성과 요약 ... 62
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