초록 ▼

○ 연구결과
- 돌나물 유전자원을 전국 61개 지역에서 총 81 지방종을 수집하였으며, 특성평가를 통하여, 8 지방종(완주, 군산, 포항, 완도, 금산, 부천, 포천, 용인)을 선발하였음.
- 질소시비량에 따른 생육 및 개화특성은 15kg/10a 질소 시비가 엽수, 마디수, 신초수 및 품질특성이 양호하였음.
- 여름철 차광재배시 돌나물의 생육은 초장, 마디수. 엽수, 줄기두께 등이 50% 차광에서 가장 양호하였고, 고미(쓴맛)도 차광율에 비례하여 낮아졌으며, 포항 지방종이 차광재배 적응성이 가장 높았음.
- 담

○ 연구결과
- 돌나물 유전자원을 전국 61개 지역에서 총 81 지방종을 수집하였으며, 특성평가를 통하여, 8 지방종(완주, 군산, 포항, 완도, 금산, 부천, 포천, 용인)을 선발하였음.
- 질소시비량에 따른 생육 및 개화특성은 15kg/10a 질소 시비가 엽수, 마디수, 신초수 및 품질특성이 양호하였음.
- 여름철 차광재배시 돌나물의 생육은 초장, 마디수. 엽수, 줄기두께 등이 50% 차광에서 가장 양호하였고, 고미(쓴맛)도 차광율에 비례하여 낮아졌으며, 포항 지방종이 차광재배 적응성이 가장 높았음.
- 담액 수경재배에 따른 초장, 엽수 등 생육특성과 무기성분 및 비타민 C 함량은 지방종간에 차이가 없었으나, 금산, 부천, 포천 등 3지방종은 엽형과 줄기두께에서 유의한 차이를 보여, 완주, 군산, 완도, 포항 등 4 지방종들이 수경재배에 적합하였음.
- 겨울철 무가온 하우스에서 수도용 육묘상자를 이용한 돌나물 재배의 적정 재식밀도는 육묘상자당 40개체였으며, 월동후 생육특성은 지방종간에 큰 차이를 보이지 않았음.
- 이상에서 금산 지방종은 잎과 줄기가 가늘고 곁가지가 잘 발달하고, 개화가 풍성하여 관상용으로 가치가 있었으며, 완주, 군산, 완도, 포항 지방종은 짙은 녹색을 띠면서 두꺼운 잎과 줄기 및 마디가 짧아 식용으로 적합하였음.
- 삽목번식에 미치는 삽수부위 및 크기는 3마디(50 mm) 정단부위에서, 배양토는 밭흙과 모래를 1:1-2:1 혼합에서 활착 및 생육이 좋았으며, 점파, 조파, 산파 간활착율은 차이가 없었음.
- 기내 대량번식을 위한 마디배양은 0.2 mg/L kinetin 첨가배지에서 신초수, 초장, 근장, 생체중 등이 가장 양호하였음.
- 캘러스 유기는 3.0 mg/L 2,4-D와 1.0 mg/L BA 첨가 배지에서 캘러스 유기율이 가장 높았으며, 식물체 분화는 0.2 mg/L NAA와 3.0 mg/L BA 첨가배지에서 가장 양호하였음.
- 식물체 분화를 위한 AgNO₃ 첨가는 15μM AgNO₃ 첨가배지에서 95%의 식물체 분화율을 보였고, 캘러스 형성은 B5>MS배지, 잎>줄기절편 순으로 양호하였으나, 식물체 분화는 MS>B5배지에서, 줄기>잎절편 순으로 높았음.
- Genotype의 영향은 캘러스 형성에는 큰 차이가 없었으나, 식물체 분화는 금산,부천, 포천, 용인 등 4개 지방종에서 42∼95%, 부안, 제주, 순천, 광양 4개 지방종은 2∼3%, 기타 20개 지방종에서는 식물체 분화가 되지 않았음.

Abstract ▼

Development of new cultivar and propagation system in Sedum sarmentosum
Sedum sarmentosum is grown in Korea, Japan and China. In Korea, S. sarmentosum is one of the favorite fresh-vegetables in the early spring season. S. sarmentosum is rich in vitamin C, and it′s extracts show the HIV inhibition

Development of new cultivar and propagation system in Sedum sarmentosum
Sedum sarmentosum is grown in Korea, Japan and China. In Korea, S. sarmentosum is one of the favorite fresh-vegetables in the early spring season. S. sarmentosum is rich in vitamin C, and it′s extracts show the HIV inhibition and hepato-protective activity. We expect that S. sarmentosum are widely cultivated for farmers as a new vegetable crop.
This work was conducted with the following objectives;
i ) To collect and to conserve the local strains of Korean native S. sarmentosum;
ii) To estimate the growth and flower characteristics of collected local strains;
iii) To investigate the growth, quality and biomass according to nitrogen fertilization, hydroponic culture, shadding rate, and the culture using rice nursery tray in non-heating plastic house;
iv) To optimize the cutting propagation of S. sarmentosum;
v) To improve the plant regeneration efficiency from callus.
1. Collection, conservation, estimation and selection of local strains in Korean native S. sarmentosum
For conservation of Korean native S. sarmentosum, total 81 local strains were collected from 61 different regions containing some islands or mountains in South Korea The collected local strains were propagated and conserved in upland field of Wonkwang University.
To estimate the growth and flowering, local strains were cultivated b.y cutting (10x10 cm, 1 ㎡/strain) on September 7th-15th, In next spring, eight local strains were selected through estimation of growth and flowering characteristics among them. Wanju and Gunsan local strains with high yield and non-flowering characteristics was favorable as an edible resource. Kemnsan, Bucheon and Pocheon local strains with abundant flower stock and branches was desirable as a garden plant, and Pohang, Wando, and Yongin local strains were excellent both of growth and flowering characteristics.
2. Growth, quality and biomass of selected local strains by culture conditions
To determine the optimum nitrogen fertilization for the production of high quality vegetable, S. sarmentusum was cultivated by cutting propagation in the pot (1/5,000a) and field experiments treated with different levels of nitrogen (0, 5, 10, 15, 20, 30 kg/10a and 0, 10, 15, 20, 25 kg/10a). In pot cultivation, plant height, number of stem, fresh weight and dry weigh were increased along with added nitrogen level, but they showed no significances in 15~30 kg/10a of nitrogen. SPAD value increased along with added nitrogen level, and it showed no significance in 10~30 kg/10a of nitrogen. The value of L measured by chroma meter showed a tendency to decrease along with added nitrogen level. Number of flower stock in Keumsan local strain was decreased according to higher nitrogen level, and it was the highest in 10 kg/10a of nitrogen. Wanju local strain was not bloom in all treatments.
In field cultivation, plant height and number of stem were increased with higher nitrogen level, but they were not significant except 0 kg/10a of nitrogen. Other characteristics in field cultivation showed no significances among nitrogen levels. Fresh weight and dry weight were linearly increased according to higher nitrogen level, they were not significant among 15~25 kg/10a of nitrogen levels. The nitrogen level for high quality and maximum growth of S. sarmentosum was 15 kg/10a. Keumsan local strain was desirable having the abundant flower stock and branches. and Wanju local strain showed higher yield and non-floweling characteristics.
In hydroponic culture for selected local strains, plant height, number of node, number of leaf and number of stem showed no significances among eight local strains. In Keumsan, Bucheon and Pocheon local strains, stem diameter and leaf shape were significantly more slender than those in other local strains. Chroma value (L, a, and b) was low in Keumsan, Bucheon and Pocheon local strains. Most mineral contents excepts Fe in hydroponic culture were higher than those in field culture. Vitamin C and mineral contents were not significant among eight local strains. Fresh and dry weight of shoot in Wanju, Gunsan and Wando local strains were also higher than those in Keumsan, Bucheon and Pocheon local strains. Accordingly, Wanju, Gunsan, Pohang and Wando local strains were optimal for the hydroponic culture, but Keumsan, Bucheon, Pocheon and Yongin local strains were not.
In the culture using rice nursery tray in non-heating plastic house, the growth reaction before or after wintering was no difference among planting density (40, 60 and 80 cuttings per tray) in cuttings, September 7th. The growth characteristics after wintering didn't show a difference among 1:3 local strains, but Keumsan, Bucheon, Pocheon and Yongin local strains were not adequate for the edible resources with high quality, because their characteristics had slender stem and succulent height.
3. Cutting propagation of S. sarmentosum4. Tissue culture of S. sarmentosum
For cutting propagation of S. samentosum, the influences of cutting part, and size were investigated. Among three part of cuttings, middle stem of cuttings showed the most effective shooting and rooting. In the size of cuttings, number of shoot, growth and soil adjustment were increased in the order one node (15 mm length)For selection of culture medium for S. sarmentosum, the effect of culture medium on cutting propagation was investigated in mixtures of upland soil, sand and carbonized rice hull. Shoot growth, rooting and root elongation were better in 1:1 or 2:1 of upland soil:sand (v/v) than in the mixtures of upland soil and carbonized rice hull.
For direct cutting propagation in field, plant height and number of shoot were no difference in space drill seeding, drill seeding and broadcast seeding, and survival rate was 97~100%. Accordingly, cutting propagation in Autumn season was no problem in survival rate to late September.
4. Tissue culture of S. sarmentosum
For in vitro node cull me, stem segments (10 mm length) were cultured on MS media supplemented with 0.0, 0.2, 0.5 and 1.0 mg/L BA or kinetin. The highest multi-shoot propagation was obtained from MS medium supplemented with 0.2 mg/L kinetin. When transferred to growth regulator free MS medium after 50 days, numerous shoots subsequently developed from shoot clusters.
To establish the system of plant regeneration from callus, the lear segments of S. sarmentosum were cultured on MS media supplemented with different levels of 2,4-D, NAA and BA. The callus induction and growth showed a good response on MS medium supplemented with 3.0 mg/L 2,4-D and 1.0 mg/L BA, but a few callus induced on the media containing NAA and BA. In plant regeneration, combination of BA and NAA promoted shoot organogenesis, and the highest frequency was obtained from MS medium supplemented with 0.2 mg/L NAA and 3.0 mg/L BA. When calli transferred to the plant regeneration medium containing 0.2 mg/L NAA and 3.0 mg/L BA, healthy shoots without hyperhydricity were continuously induced as 17.2 plantlets per callus after 50 days of culture. When regenerated plantlets were transferred on hormone-free MS medium for rooting and the further growlh of plantlets, rooting easily achieved from all of them.
Plantlet regeneration showed the highest frequency of 95% with the average of 6.6 plantlets per callus when 15μM AgNO₃ was added to MS medium with 0.2 mg/L NAA and 3.0 mg/L BA.
Buth leaf and stem explants, higher rule of callus induction and growth were obtained on B5 than MS medium, but plantlet regeneration was more increased on MS than B5 medium. Callus grovvth from stem explant was more promoted than leaf explant, but plantlet regeneration was appeared in opposition to callus growth.
No recalcitrant genotype showed in callus induction. Only 8 of 28 genotypes tested regenerated plantlets, and 4 genotypes produced higher plantlets (42~95%), 4 produced a few plantlels (2~3%), and 20 did nol produced plantlels at all. Keumsan local strain showed the highest plantlet regeneration, followed by Bucheon, Pocheon and Yongin local strains.
From above results, Keumsan local strain with slender stems and leaves, plenty branches and flowers was adequate as a ornamental or ground cover resource. Wanju, Gunsan, Pohang and Wando local strains with thick stems and leaves, short nodes, and dark green leaves were adequate for high quarlity of edible resources. Especially, Wanju and Gunsan local strains with non-flowering charactcristics were optimal for the cultivation from late spring to summer, Pohang local strain for the shading cultivation, and Wanju, Gunsan, Pohang and Wanju local strains for the hydroponic cultivation.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 요약문 ... 4
• SUMMARY ... 11
• CONTENTS ... 15
• 목차 ... 17
• 제 1 장 연구개발과제의 개요 ... 19
• 제 1 절 연구개발의 목적 ... 19
• 제 2 절 연구개발의 필요성 ... 19
• 제 3 절 연구개발의 내용 및 범위 ... 20
• 제 2 장 국내외 기술개발 현황 ... 22
• 제 1 절 국내 돌나물 연구 현황 ... 22
• 제 2 절 국외 돌나물 연구현황 ... 23
• 제 3 절 돌나물 연구의 문제점 ... 24
• 제 3 장 연구개발 수행 내용 및 결과 ... 25
• 제 1 절 돌나물 유전자원 수집 및 우량계통 선발 ... 25
• 1. 서언 ... 25
• 2. 재료 및 방법 ... 26
• 3. 결과 및 고찰 ... 27
• 가. 유전자원 수집 및 증식유지 ... 27
• 나. 수집 지방종의 생육 및 개화 특성 ... 30
• 다. 우량계통 선발 ... 36
• 4. 결론 ... 39
• 제 2 절 선발계통들의 생육 및 품질 특성 ... 42
• 1. 서언 ... 42
• 2. 재료 및 방법 ... 43
• 3. 결과 및 고찰 ... 47
• 가. 질소시비수준에 따른 생육 및 품질 특성 ... 47
• 나. 차광처리에 따른 품질 및 생육 특성 ... 56
• 다. 담액 수경재배에 따른 생육 및 품질 특성 ... 62
• 라. 수도용 육묘상자를 이용한 무가온 비닐하우스 재배 ... 68
• 3. 결론 ... 80
• 제 3 절 삽목에 의한 돌나물 대량번식 체계 확립 ... 86
• 1. 서언 ... 86
• 2. 재료 및 방법 ... 87
• 3. 결과 및 고찰 ... 88
• 가. 삽목번식에 미치는 삽수 크기 및 부위의 영향 ... 88
• 나. 삽목번식에 미치는 배양토의 영향 ... 89
• 다. 가을 삽목번식시 재식밀도 및 파종방법에 따른 월동전 생육 특성 ... 92
• 3. 결론 ... 94
• 제 4 절 조직배양에 의한 돌나물 번식체계 확립 ... 95
• 1. 서언 ... 95
• 2. 재료 및 방법 ... 96
• 3. 결과 및 고찰 ... 99
• 가. 기내 마디배양에 의한 대량번식 ... 99
• 나. 캘러스 배양에 의한 대량번식 ... 101
• 3. 결론 ... 115
• 제 4 장 기대효과 ... 118
• 1. 기술적 측면 ... 118
• 2. 경제.산업적 측면 ... 118
• 제 5 장 연구개발결과의 활용계획 ... 119
• 제1절 추가연구의 필요성 및 전망 ... 119
• 제2절 결과 활용계획 ... 119
• 제 6 장 참고문헌 ... 120
• 끝페이지 ... 126