보고서 정보
주관연구기관 |
목포대학교 Mokpo University |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2011-11 |
과제시작연도 |
2010 |
주관부처 |
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
등록번호 |
TRKO201400026377 |
과제고유번호 |
1545002028 |
사업명 |
수산기술개발 |
DB 구축일자 |
2014-11-14
|
초록
○ 연구결과
- 넓미역 개체군의 생태 및 생리학적 특성을 파악하여 완전 양식을 위한 기초자료 확보
- 넓미역의 유성 및 무성생식방법을 이용한 인공종묘 생산 기술 확립
- 넓미역의 완전양식을 위한 적정 가이식 및 양성 기법의 확립
- 넓미역의 고부가가치적 이용을 위한 추출물의 피부 미백,노화방지 효과 파악
- 넓미역 양식을 통한 양식어가의 수익률 증대 효과 분석
Abstract
▼
The brown alga Undariopsis peterseniana (Kjellman) Miyabe et Okamura only occurs in the Udo Strait between Udo Island and Jongdalri, Jeju Island in Korea (Lee 1998) although Kang (1966) reported the alga growing in Kapado as well as Udo Strait. The areas inhabited by this alga are directly affected
The brown alga Undariopsis peterseniana (Kjellman) Miyabe et Okamura only occurs in the Udo Strait between Udo Island and Jongdalri, Jeju Island in Korea (Lee 1998) although Kang (1966) reported the alga growing in Kapado as well as Udo Strait. The areas inhabited by this alga are directly affected by the Tsushima warm current. Within this area, the algae is reported as occurring within Laminaria japonica beds at the depth of 15 m (Lee and Koh 1991), and in areas where gravel-form nonarticulate coralline algae cover the sea bed (Lee 1998).
U. peterseniana has traditionally been used as a salad vegetable in Jeju Island, but recently the natural populations of this alga is in danger of disappearing. The Korean government tries to protect marine algal diversity and this alga is one of the species protectedby the law in Korea (Ministry of Government Legislation, MGL 2010).
Recently, the growth and maturation period of U. peterseniana has been studied by Hwang et al. (2010). U. peterseniana has been described as a warm water species in the Order Laminariales (Kirihara et al., 2006). In other areas of Korea, populations of cool water kelps are declining. This decline has been partly attributed to increasing seawater temperatures around the Korean peninsula in the last decade (KHOA, 1997-2006). Associated with the increasing water temperatures is a change in the seaweed species composition found in the area. As U. peterseniana is a warm water species, it has been suggested that it may be a candidate for aquaculture in areas where environmental warming is occurring.
We report here the relationships between environmental conditions during whole growth period for the artificial seed production and mass-cultivation of U. peterseniana. Those results are followed:
1. Ecological study of Undariopsis peterseniana
Ecological survey was conducted from January to December 2008 in Udo, Jeju Island. Natural population of U. peterseniana in Udo was distributed at 12 to 17 m of water depths in the narrow strait between Byyangdo and Udo. The area of the population estimated 228 ha at their maximum growth period. Among 5 survey points, U. peterseniana population was observed just in Udo. The U. peterseniana population formed dense patches at depths of 12~17 m depth.
Growth in length of the alga reached a maximum of 168±36cm at June. Sorus were observed from May to August, and a maturation peak was detected in June, when bottom seawater temperatures were around 18℃. After zoospore release, the alga bleached and disappeared after August. Developmental initiation of sporophytes occurred at temperature above 15℃, and sorus formation required approximately 236 degree-days.
2. Physiological study of Undariopsis peterseniana
Photosynthetic yield of U. peterseniana was higher value in vegetative blade than sorus area. Environmental conditions for the alga was different according to their developmental stages. Gametophyte growth facilitated at 15-25℃, 20-30 μmol m-2 s-1. Micro sporophyte growth facilitated at 20℃, 60 μmol m-2 s-1 and 10:14h (L:D) conditions. Young blade of the alga grew faster under 20-25℃ 20 μmol m-2 s-1 and 10:14h (L:D) conditions than others. Induction of regeneration and maturation of the free-living gametophytes of U. peterseniana was studied at four temperatures (5, 10, 15, and 20℃), four levels of irradiance (5, 10, 20, and 40 μ mol m-2 s-1) and three photoperiods (14:10, 12:12, and 10:14h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of 15℃, 20 μmol m-2 s-1, 10:14h (L:D), whereas the conditions for male gametophytes were slightly different at 15℃, 20 μmol m-2 s-1, 14:10h (L:D). The sexual maturation of female and male gametophytes took place under 15℃, 20 μmol m-2 s-1, 14:10h (L:D) and 10℃, 10 μmol m-2 s-1, 10:14h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for artificial seed production of U. peterseniana.
3. Cultivation study of Undariopsis peterseniana
Growth and maturation were investigated from December 2008 to October 2009 in their natural habitat. Zoospores were transplanted into an intensive seaweed culture ground in Wando, southern coast of Korea, for the mass cultivation of this species. Indoor and outdoor cultivation were conducted from June 2008 to May 2009. Mean production obtained from the zoospore seeding was 31.1±1.5 kg wet wt. m-1 of culture rope during the cultivation period in situ. Transplanted F1 thalli in Wando showed that length was 1.7 times longer than their parents in Udo and their maximal growth period changed from June (at 19.9℃ in Udo of natural habitat) to April (at 14.0℃ in Wando of culture ground). The relationship between optimal water depth for culture and underwater irradiance during the U. peterseniana cultivation was defined as: y=-0.78X + 7.67 (r2=0.92). This study indicates that U. peterseniana could be successfully transferred to the northern coast beyond the original habitat in Jeju Island. We cultured a hybrid kelp consisting of a cross between free-living gametophytes of U. peterseniana and U. pinnatifida in an attempt to extend culture period of Undaria which is an important species for both the abalone industry and for commercial seaweed mariculture for human food applications. Morphological characters and cultivation period were compared between the parent thalli and the hybrid. The cultivation experiment was conducted in Wando, on the southern coast of Korea (34°26´18.68″N, 127°05´43.88″E). The morphological characteristics of the hybrid thalli were intermediated between the two species having shallow pinnated blades and a reduced reproductive organ.
Hybrid thalli showed faster growth rates, 1.5 times greater biomass and a longer cultivation period than the parent thalli. The hybrid strain possessed characteristics that indicate it could be used as an alternative kelp source to supply the abalone feed industry.
4. Utilization and Economical evaluation
In order to investigate whether or not CCD-986sk cell line can be affected by U. peterseniana extract, we examined the MTT assay when we treated Korean U. peterseniana extract in CCD-986sk human fibroblast cell line.
80% methanol extract samples of U. peterseniana were tested for cell proliferation activity by means of a modification of the MTT assay.
U. peterseniana extracts showed significantly strong cell proliferation activity between 50mg/ml - 3.13mg/ml compared with control group. Especilly 50mg/ml (O.D.=0.166±0.001) showed powerful cell proliferation activity more than 251 percent compared with the control group. These results suggest that Korean U. peterseniana extract could be an excellent candidate for protection of human skin aging.
From 20 set of installation (100 m of long-line culture rope), ca. 60 tons-wet wt of U. peterseniana can be produced. Total sale of the production is 180,000 thousand won and production cost is 28,400 thousand won, thus the net profit is 151,600 thousand won. The profit rate of U. peterseniana cultivation is 84.22%.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.