초록 ▼

○ 연구결과
•효소를 이용하여 poly(catechin)으로 전환함으로써 고부가가치 식품 소재 개발
- (+)/(-)-catechin 표준물질을 기질로 이용하여 laccase 산화중합에 의한 중합체 생산 최적 효소반응조건을 검토한 결과, (-)-catechin에서는 pH 5.0, 온도 50℃에서 중합체 상당 침전물의 생성율이 우수하였으며 (+)-catechin의 경우에는 30℃의 최적온도와 pH 5.0에서 최적 형성율을 나타내었고, 첨가 효소량에 대해서는 농도의존적이었고 기질첨가량에서는 반응액 대비 1.0% 이상에서 현저

○ 연구결과
•효소를 이용하여 poly(catechin)으로 전환함으로써 고부가가치 식품 소재 개발
- (+)/(-)-catechin 표준물질을 기질로 이용하여 laccase 산화중합에 의한 중합체 생산 최적 효소반응조건을 검토한 결과, (-)-catechin에서는 pH 5.0, 온도 50℃에서 중합체 상당 침전물의 생성율이 우수하였으며 (+)-catechin의 경우에는 30℃의 최적온도와 pH 5.0에서 최적 형성율을 나타내었고, 첨가 효소량에 대해서는 농도의존적이었고 기질첨가량에서는 반응액 대비 1.0% 이상에서 현저히 저하됨을 확인하였음. 녹차로부터 제조된 시판 catechin류 혼합제품의 경우에도 30~50℃, pH 5~7의 최적 반응조건을 보였고, 효소와 기질 첨가량의 경우에는 catechin 표준물질과 유사한 경향을 나타내었음. 반응시간에 따른 영향은 큰 차이를 보이지 않았고 항산화 활성에서는 모든 중합체가 기질보다 증진되지는 않았으나 (+)-catechin과 시판 catechin 제품의 중합체에서는 유의적으로 우수한 항산화 활성을 나타내었음
- 녹차로부터 제조된 시판 catechin류 혼합제품을 기질로 laccase로부터 생성된 중합체 상당 물질의 식품 및 cosmeceutical로의 소재 활용성을 검토하기 위하여 SD rat를 이용한 급성 및 아급성 독성 평가를 진행한 결과, 체중변화 및 식이섭취량에서 무첨가군과 유의적인 차이를 나타내지 않았으며 전혈을 이용한 hematological parameter와 다양한 혈액학적 지표의 측정에서도 유의적인 차이를 보이지 않았고, 간/신장/비장 등의 장기무게에서도 유의적인 차이가 확인되지 않았고 간과 신장의 HE stainin을 이용한 병리학적 관찰에서도 무첨가군과 유의적인 차이를 보이지 않아 중합체 상당침전물은 안전한 것으로 판단되었음
- 이와 같이 독성평가에서 안전한 polycatechin 소재를 화장품에 첨가한 후 기능성 화장품 소재로서의 활용가능성 평가하기 위하여 정상적인 피부를 가진 중년여성에게 피부미용 효과를 관찰하였음. 6주 관찰 결과, 중합체 상당 침전물이 첨가된 화장품은 수분, 경표피 수분손실량, 멜라닌지수나 홍반지수는 대조군인 base 화장품과 유의적인 차이를 보이지 않았으나 감소하는 경향을 나타내었고, 멜라닌 지수는 polycatechin 화장품군에서 유의적인 차이를 보여 polycatechin은 미백의 기능성 화장품의 소재로써 적용하기에 가능성이 높은 소재임을 알 수 있었음
•효소처리에 의해 면역활성이 우수한 다당체를 분리 및 소재화
- 녹차 성숙잎 단순 열수추출물로부터 조다당 획분(GTW)과 녹차 성숙잎의 pectinase 처리 추출물에서 획득한 조다당 획분(GTE-0)을 조제하고 GTE-0로부터 44 kDa의 GTE-I과 16 kDa의 GTE-II로 분리하였으며, GTE-I의 경우 GTE-II보다 보체계에 대하여 농도 의존적으로 높은 활성을 나타냄. 이러한 다당 획분은 macrophage에 대하여 어떠한 세포독성도 나타내지 않으면서 비장세포에 대해 높은 증식활성을 보였으며 IL6 및 IL12에 대해 생산 증가능을 보였음. GTE-I과 GTE-II를 정맥주사하여 높은 NK cell 활성을 보였으며, 저용량으로도 암전이 억제활성에 효과를 보였음.GTE-I의 항전이 활성이 주로 NK cell의 활성화에 기인함과 대조적으로 GTE-II는 NK cell 및 macrophage 모두의 활성화를 유도함에 기인하는 것으로 보임. In vivo 효과에서도 2 시료 모두 농도의존적으로 높은 NK cell 활성화를 보여 GTE-0는 NK cell을 경유하여 종양의 예방이나 치료에 효과적일 것으로 예견되었음
- 강력한 NK cell 활성과 항전이 활성을 갖는 GTE-II의 구조적 특성은 자연계에서는 희귀한 구성당을 포함한 15종의 당류로 구성되어 있었으며, pectin 유래의 rhamnogalacturonan-Ⅱ와 유사 구조를 갖는 다당체임을 추정할 수 있었음. 산 가수분해와 효소분해를 통하여 전체 구조를 해명한 결과, α-(1→4)-galacturono-oligosaccharide 주쇄에 Rhap-(1→5)-Kdo, Araf-(1→5)-Dha, AceA containing nonasaccharide 및 uronic acid rich oligosaccharide 분지를 갖고 있는 RG-Ⅱ와 유사한 구조로 존재함을 확인하였고, pH 1.6 이하의 조건에서 분자량 9.3 kDa의 주 peak가 분자량이 약절반인 5.5 kDa의 peak로 이동하는 현상을 보여 면역활성다당인 GTE-II는 동일 크기의 2개의 subunit로 존재하는 RG-Ⅱ dimer 구조임을 최종 확인하였음
- 등외품 녹차 유래 활성 다당의 안정성 및 응용성을 검토하기 위해 GTE-0를 조건 별 열처리하고 잔존 생리활성을 측정한 결과, GTE-0 용액의 습열처리에서 항보체 활성은 거의 변화하지 않았으나 건열처리 조건에서는 온도와 시간이 증가할수록 시간이 증가할수록 활성은 감소하는 것으로 나타났음. 또한 GTE-0 용액의 건조방법에 따른 면역활성의 차이는 극미하여 양호한 열안정성과 용해도의 특성을 보여 식품 제조용 소재로 사용되는데 문제가 없다고 판단되었고, 이를 이용한 중간제품인 powder와 granule을 제조한 결과 식품공전 상의 기준을 충족하였음
•녹차의 활성 성분인 catechin류를 효소전환기법을 이용하여 생리활성, 피부 투과율 및 장 투과율이 우수한 catechin 전환 소재 개발
- Viscozyme L을 녹차추출물에 1% 비율로 첨가하여 40°C에서 2시간 반응시켰을 때 카테킨류 추출이 가장 효율적이었음
- Viscozyme L 추출물에 다시 tannase를 0.125% 비율로 첨가하여 pH 4.5, 35°C에서 20분간 반응시 켰을 때 EGCG와 ECG가 EGC와 EC로 완전히 전환되었으며, 이와 같이 얻은 tannase-처리 녹차 추출물은 항산화 능력과 함께 피부투과율도 우수하였음
- Tannase-처리 녹차추출물이 첨가된 녹차화장품은 pH 변화가 거의 없으면서 침전도 발생되지 않아서 안정성이 높은 것으로 판단되었고 항산화력 및 tyrosinase 활성저해효과도 우수하였음
- Tannase-처리 녹차추출물이 첨가된 녹차화장품은 우수한 산화적스트레스 억제 능력을 보여 광보호 효과가 있다고 판단되었으며 주름 등급을 측정한 결과에서도 가장 낮은 주름 등급을 나타내었고 항산화 및 미백과 주름개선 효과에서도 높은 활성을 나타내어, 기능성 화장품 소재로서의 활용가능성은 대단히 높을 것으로 판단됨
- 또한, tannase-처리 녹차추출물이 첨가된 녹차화장품은 수분조절능력, 미백효과, 홍반억제 및 주름 개선 효과가 우수하였음
- 이상의 결과로부터, tannase-처리 녹차추출물이 첨가된 녹차 화장품은 in vitro와 in vivo 실험에서 항산화 능력, 주름개선 및 미백효과 등을 나타내어 피부건강을 위한 기능성 화장품 소재로서의 이용 가치가 대단히 우수할 것으로 기대함

Abstract ▼

■ Part 1 : High value-added polycatechin development using enzyme from green tea catechin catechin, an ingredient of green tea, belongs to the flavoids which is one of the most numerous and best-studied groups of plant polyphenols, In general, the duration of activity of flavonoids is known to be li

■ Part 1 : High value-added polycatechin development using enzyme from green tea catechin catechin, an ingredient of green tea, belongs to the flavoids which is one of the most numerous and best-studied groups of plant polyphenols, In general, the duration of activity of flavonoids is known to be limited to only a few hours in the body, and several flavonoids have been shown to act as pro-oxidants and generate reactive oxygen species, such as
H₂O₂. In contrast, a relatively high-molecular fraction of plant polyphenols has been reported to exhibit enhanced physiological properties and a relatively longer circulation time in vivo. High-molecular-weight plant polyphenols have also been reported to show no pro-oxidant effects. Therefore, We have synthesized the catechin polymers, in consideration of the enhanvement of antioxidatvie effects of catechin. The present study focused on the optimal reaction conditions in laccase-catalyzed polymerization of (+)/(-)-catechin and the commercial catechins products (CCP) mixture of polyphenol compounds) without using H₂O₂ in aqueous organic solvents for development of polymeric antioxidants.
The laccase-catalyzed polymerization of (+)/(-)-catechin substrate was carried out in a mixture of acetate buffer (pH 5.0) and polar organic solvent (95:5%, v.v) whereas CCP was reacted with laccase in only buffer solution. After polymeric precipitates formed during the reaction were collected by centrifugation, the resulting polymer was soluble in dimethyl sulfoxide (DMSO) and analyzed by IPLC or UV seanning method. The optimal reaction tcmperature and pH of (+)-catechin was 30℃ and pH 5.0, and formation of polymeric precipitates was increased in proportion to the amount of enzyme. The (+)-catechin addition of 0.5% (w/v, to the total reaction volume) was also optimized in 1.5% (w/v, to the total reaction volume as protein amount) of enzyme concentration. Although antioxidant activities of these polymeric precipitates were lower than catechin substrate, precipitates polymerized from (+)-catechin with laccase had the potent antioxidant activity. Meanwhile, polymeric precipitate was maximal recovered at 30~50℃ and pH 5~7 in CCP substrate, and increased at enzyme amount over 1.5% (to the reaction volume as protein) and decreased at substrate amount over 1.0%. When polymeric precipitate formed under the optimal reaction condition was analyzed by HPLC, the new peak, which did not detected in substrate or enzyme control as well as the supernatant of reaction mixture, was formed in the late retention time. These polymeric precipitate alco showed the potent antioxidative effect despite lower than the substrate. These results suggested that catechin was oxidatively polymerized by laccase to give a new class of flavonoid polymers.
In addition, polymeric precipitates, which was produced from CCP with laccasse, were analyzed to identify polymeric degrees and their structures by ¹H-NMR and MALDI-TOF. Polymeric precipitates could not correctly analyzed because CCP substrates contained various kinds of catechin compounds and mechanism of oxidative polymerization was not ully known. However, it was confirmed that the analysis patterns of polymeric precipitates were different from those of CCP substrate. Especially, subfractions fractionated by HP-20 contained more high-molecule-weight compounds than CCP substrate in MALDI-TOF analysis. Moreover, as expected, non-polarity subfraction eluted by 95% EtOH consisted mainly of high-molecule-weight compounds. Therefore, these results suggest that polymeric precitates might be comprise polymer of catechins through the oxidatory polymerization by laccase.
To utilized polycatechin as cosmeceutical material, polycatechin (5,000 mg/kg) was orally administered for 2 weeks using SD rat in acute toxicity examination, and 1,000 mg/kg for subacute toxicity. There IS no significant difference between control and polycatechin-administered rats in body weight gain and daily intake. Hematological parameters and blood biochemical parameters of polycatechin-administered rats was not also significantly different with saline-administered group, and no significant difference between male and female. In addition, the relative organ weights such as liver, kidney and spleen, of SD rat administered with polycatechin did not show the significant difference against the control, and there is no significant difference in representative microscopic finding in the liver or kidney by HE staining. These results suggest that polycatechin was safe in oral administration.
Finally we investigated the influence of cosmetic formulations prepared with polycatechin from CCP with laccase on skin in human. This interaction of base cosmetic formulation and polycatechin-supplemented cosmetic with skin was evaluated by non-mvaSlve biophysical techniques using the Comeometer, Tewameter, Mexameter and Skin-Visiometer measuring the skin water content, skin barrier function, melanin, erythema value and skin wrinkles, respectively. Total forty-four women, twenty-two women completed a 6 wks trial of polycatechin-supplemented cosmetic applied twice daily to the eyelid and the base group applied to the eyelid. Skin water contents, skin barrier function, melanin, erythema and wrinkles of the polycatechin group improved or reduced than base group despite no significant difference. In conclusion, a cosmetic with polycatechin could be used as a functional cosmetic, cosmeceutical with not only anti-oxidant, whitening and anti-aging effects, but also the improvement of skin water contents, skin barrier function, anti-wrinkles and the reduction of pigmentation and erythema.
■ Part 2 : Characterization of immunostimulating polysaccharide from off-grades of green tea and development of functional food materials
Tea, from the plant Camellia sinensis L., is one of the most popular beverages consumed worldwide in its green, black, oroolong form. It contains many compounds such as polyphenols, polysaccharides, vitamins, and so on, and it reduces the risk of a variety of iseases. Tea polyphenols are the main components in tea extracts. Epigallocatechin gallate (EGCG), a major polyphenolic compound in tea extracts, is well-known to have antioxidant, antibacterial, immunomodulatory and antitumor activities. Numerous data involving above results have been indicated that the biological and pharmacological effect of tea was focused on the EGCG in immature tea leaves. However EGCGs can not account for all of the ingredients in tea and their observed clinical effects. The possibility that other biologically active ingredients such as specific polysaccharides may exist in mature tea leaves, still remains. In order to develop the new physiologically active polysaccharides from mature tea leaves, the polysaccharides were isolated from pectinase digests of mature tea leaves and their immuno stimulating activity were examined.
The crude polysaccharide fraction (GTW) and polysaccharide fraction (GTE-D) treated with commercial pectinase were prepared from the leaves of C. sinensis, and their immuno-stimulating activities were compared witheach other. The crude polysaccharide fraction(GTE-0) in pectinase digest of mature tea leaves was purified by one column chromatography on Sephadex G-100 to give the two purified polysaccharides, GTE-I and GTE-II with different molecular weights. GTE-I and GTE-II were eluted as almost single HPLC peaks and their molecular weights were estimated to be 44 kDa and 16 kDa, respectively. The high molecular weight polysaccharide, GTE-I showed a higher anti-complementary activity than GTE-II in a dose-dependent fashion. Also the anti-complementary activity of GTE-0 was more optent than that of GTW. Results obtained by crossed immunoelectrophoresis using anti-human C₃ in the absence of Ca⁺⁺ ion suggested complement activation by GTE-I and GTE-II from tea leaves occurs via both alternative and classical pathways. In an in vitro cytotoxicity analysis, all tea polysaccharides did not affect the growth of murine macrophages whereas showed high splenocyte proliferating activity. Peritoneal macrophages stimulated with GTE-I and GTE-II produced various cytokines such as IL-6 and IL-12 and their activities were higher than GTW fraction. In an assay for natural killer (NK) cell activity, i.v. administration of GTE-I and GTE-II significantly augmented NK cytotoxicity against Yac-1 tumor cells. In experimental lung metastasis of B16-BL6 melanoma cells, prophylactically intravenous(i.v.) administration of GTE-I and GTE-II purified from the pectinase digest of mature tea leaves, significantly inhibited lung metastasis at a dose of 100 μg/mouse. Also the depletion of NK cellsby rabbit anti-asialo GMI mostly or partly abolished the inhibitory effect of GTE-I and GTE-II on lung metastasis. The macrophages obtained from the mice which were injected with GTE-II (100 μg/mouse) 3 days before the assay showed significantly higher tumoricidal activity against tumor cells than that of the untreated macrophages. These data suggest that the anti-metastatic activity of GTE-I is mainly associated with activation of NK cells whereas GTE-II with activation of both NK cells and macrophages.
In vivo effect of two polysaccharide fractions, GTW-0 and GTE-0 from green leaves on the natural killer cell activity and anti-metastatic activity was studied. Oral administration of GTW-0 and GTE-0 induced the activation of NK cells in a dose-dependent manner. Prophylactic oral administration (p.o.) of GTW-0 and GTE-0 significantly inhibited metastasis in experimental lung metastasis of B16-BL6 melanoma. However, oral administration of GRE-0 was more effective than that of GTW-0 at the same dosage. Therefore, we suggest that the administration of GTE-0 may by effectivel for preventing and/or treating cancer through NK cell activation. However, further studies are needed to elucidate the possible mechanisms of the anti-metastatic activity and to investigate the other beneficial effects of GTE-0 for the development of a new biological response modifier.
From these results, one can conclude that mature tea leaves contain the polysaccharides in addition to healthy components, and these polysaccharides appears to improve immune-stimulating and anti-metastatic activities beneficial to human health. To develop as ingredients of functional foods from mature tea leaves, it is necessary to introduce pectinase-treated process for mature tea leames.
GTE-II withpotent NK-cell activity and anti-metastatic activity has been structurally characterized. GTE-II consisted of 15 different monosaccharides whichincluded rarely observed sugars, such as 2-0-methyl-fucose, 2-0-methyl-xylose, apiose, aceric acid, 9-deoxy-D-manno-2-octulosonic acid(Kdo), and 3-deoxy-D-lyxo-2-heptulosaric acid(Dha). Methylation analysis indicated that GTE-II comprised 22 different glycosyl linkages such as 2,3,4-linked Rha, 3,4-linked Fuc and 3'-linked Api, being characteristic of rhamnogalacturonan II(RG-II). The primary structure of GRT-II was elucidated by sugar composition, methylation analysis and oligosaccharide sequence analyses using GC, GC-MS, ESI-MS and ESI-MS/MS. Sequential degradation using partial acid hydrolysis indicated that GTE-II contained Rhap-(1→5)-Kdo and Araf-(1→5)-Dha structural elements, AceA-containing nonasaccharide and uronic acid rich oligosaccharide chains in addition to an α-(1→4)-galacturono-oligosaccharide chain. HPSEC indicated that GTE-II mainly comprised RG-II of higher molecular mass (9.3 kDa) and that the changes of molecular mass to apparent 5.5 kDa under less than pH 1.6 were occurred. From above results, it was inferred that the anti-metastatic polysaccharide in green tea leaves, GTE-II was mainly present as a RG-II dimer.
For the application and stability of GTE-0, changes of the anti-complementary activity durin food processing procedures of GTE-0 was investigated. The anti-complementaru activity after steaming of GTE-0 was not changed significantly comparing with untreated group. Residual activity after dry heat treatment of GTE-0 was decreased than that of untreated group at higher temperature and longer treating time. Drying method did not affect the anti-complementary activity of GTE-0. Powder and granule type intermediate products with GTE-0 from tea leaves were produced and meet the standard from Food Addendum.
■ Part 3 Enhancement of physiological activities of green tea catechin by removal of gallate and its utilization
The objectives of this study were to develop extraction processes of catechins from green tea leaves and to evaluates the possibility of it's application for anti- oxidative, whitening and anti-wrinkles functional product.
In first study, green tea leaves were hydrolysated by several different kinds of cellulase which breaks internal bonds to disrupt the crystalline structure of cellulose and expose individual cellulose polysaccharide chains to increase its extraction efficiency. We attempted to set the practical optimal conditions for the enzymatic determination on green tea extraction. High-performance liquid chromatography (HPLC) was used for the screening and quantitative analysis for catechins in green tea. Tannase was then added to green tea extract for the bioconversion of catechins, a tannin peculiar to green tea. We concluded that tannase could increase powerful catechins contents in green tea and that the higher physiological activity of green tea make it beneficial for protecting the body from oxidative damage due to free radicals. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total polyphenols and green tea hydrolysate by Viscozyme L was higher than other commercial enzymatic treatments (p<0.05). Therefore, tannase concentrations ranging from 0.125%, were used to initiate reactions at pH 4.5, at temperature of 35°C, extract time for 20 min. It was verified that tannase-converted green tea extracts (TG) have DPPH, 2.2-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging higher activities total polyphenols, flavonoids contents and skin permeation than normal green tea extracts (NG) and green tea hydrolysate by Viscozyme L (VG). The results of this study indicated that TG was very suitable for making cosmetic formulations.
The aim of second study was to investigate the anti-oxidant activities, whitening and anti-aging effect of containing TG. DPPH, ABTS, hydroxyl radical scavenging activity, measurements of reducing power, collagenase, elastinase inhibition activity assay and tyrosinase inhibition activity assay were carried out. The measurement of skin permeation by Franz-type diffusion cell was performed. Oxidative damage was made by irradiating ultraviolet B (UVB) on the skin of hairless mouse. In order to compare the inhibition of oxidative stress, gluthathione reduced form (GSH), hydrogen peroxide and lipid peroxide, of the skin of hairless mouse which were exposed to UVB 3 times a day for 7 days were measured.
The results are follows as; the pH of five cosmetic formulations are fitted, which is range of skin surface pH, has to be stable. DPPH, hydroxy radical ICso value the TGE (cosmetic 5% tannase-converted green tea extract containing in base essence) was significantly superior to the NGE (cosmetic of 5% normal green tea extract containing III base essence) (p<0.05). ABTS IC!» value and the reducing power of the ArE (cosmetic of 2% arbutin containing in base essence) were significantly higher than others (p<0.05). TGE showed the inhibition of collagenase and tyrosinase activities. If could be regarded as a anti-aging and whitening cosmetics. Skin permeation of TGE was significantly higher than the BsE (basic ingredients containing in base essence), NGE, AdE (cosmetic of 0.04% adenosine containing in base essence) and ArE, (p<0.05). The skin, oxidative damage was made by irradiating UVB on the skin of hairless mouse. As a result of GSH of TGE was significantly higher than No UVB (p<0.05). There was no significant difference in hydrogen peroxide and lipid peroxidation between TGE and No UVB. Therefore, TGE can be considered that it has photoprotective effect.
The third main objective of the present study was to investigate the influence of cosmetic formulations prepared with green tea from different extract condition (NGE and TGE) on skin in human. This interaction of green tea and tannase-converted green tea with skin was evaluated by non-invasive biophysical techniques using the Comeometer, ewameter, Mexameter and Skin-Visiometer measuring the skin water content, skin barrier function, melanin, erythema value and skin wrinkles, respectively. Total forty-four women, twenty-two women completed a 8 wks trial of TGE applied twice daily to the eyelid and the other group NGE applied to the eyelid. Skin water contents (pIn conclusion, a cosmetic with TGE could be used as a functional cosmetic, cosmeceutical with not only anti-oxidant, whitening and anti-aging effects, but also the improvement of skin water contents, skin barrier function, anti-wrinkles and the reduction of pigmentation and erythema.

목차 Contents

• 표지 ... 1
• 제출문 ... 3
• 요약문 ... 3
• SUMMARY ... 14
• CONTENTS ... 21
• 목차 ... 22
• 제 1 장 연구개발과제의 개요 ... 24
• 제 1 절 연구개발 대상 기술의 경제적 중요성 ... 24
• 제 2 절 연구개발 대상 기술의 기술적 중요성 ... 25
• 1. 녹차 다당 연구의 필요성 ... 25
• 2. 녹차 catechin류의 Gallate 제거에 의한 생물전환 연구의 필요성 ... 27
• 3. 녹차 카테킨의 polymer 전환 연구의 필요성 ... 29
• 제 3 절 연구개발 대상 기술의 산업적 필요성 ... 30
• 1. 국내 차 산업 부진으로 차 재고 활용 시급 ... 30
• 2. Catechin류 기능성을 이용한 다양한 산업에서 적용성과 남는 자원 활용 ... 31
• 제 2 장 국내외 기술개발 현황 ... 32
• 제 1 절 국내외 관련분야 기술개발 현황 ... 32
• 1. 녹차다당의 면역증진 ... 32
• 2. Tannase를 활용한 녹차 추출기술 ... 32
• 3. Laccase 생산 균주를 활용한 녹차 추출기술 ... 32
• 4. Poly(catechin)을 이용한 고부가가치 제품 생산을 위한 녹차 가공기술 ... 33
• 제 2 절 기술개발 현황분석을 통한 연구추진계획 ... 33
• 제 3 장 연구개발수행 내용 및 결과 ... 35
• 제 1 절 효소에 의한 녹차 카테킨의 polymer 전환 및 소재화 ... 35
• 1. 연구개요 ... 35
• 2. Laccase의 중합체 생성조건 최적화 ... 38
• 3. Poly catechin의 안전성 평가 ... 76
• 4. Poly catechin이 첨가된 피부미용 화장품 시제품의 인체적용 평가 ... 85
• 제 2 절 등외품 녹차로부터 면역활성 다당체 규명 및 소재화 ... 94
• 1. 등외품 녹차로 부터 면역활성 다당체의 분리 및 정제와 정제 다당체의 면역호라성 평가 ... 94
• 2. 등외품 녹차 유래 활성 다당체의 구조 규명 및 응용성 검토 ... 128
• 제 3 절 녹차 카테킨 gallate 제거에 의한 생리활성 증대와 소재화 ... 156
• 1. 탄나아제를 이용하여 생물전환시킨 녹차 카테킨의 생리활성 효과 ... 156
• 2. 탄나아제로 생물전환시킨 녹차화장품의 생리활성 ... 176
• 3. 결론 ... 208
• 제 4 장 목표 달성도 및 관련분야에의 기여도 ... 209
• 제 5 장 연구개발 성과 및 성과활용 계획 ... 210
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 214
• 제 7 장 참고문헌 ... 216
• 끝페이지 ... 229