초록

Ⅳ. 연구개발결과
○ 국외 자료 분석을 통한 사료 내 곰팡이독소의 샘플링법 확립
○ 국내 미관리 곰팡이독소 3종(데옥시발레놀, 제랄레논, 푸모니신)에 대한 분석법 확립
○ 사료 관리 단계별 곰팡이독소 오염 실태 조사 및 중점관리기준점 설정
○ 닭, 돼지에 대한 사료 곰팡이독소 급여에 따른 생산성 및 건강성 저해 효과 구명
○ 닭, 돼지에 대한 곰팡이독소 저감제 활용 기술 확립

Abstract ▼

A possibility of mycotoxin contamination in feed production, storage and transportation has increased due to recent climate change. Since international grain prices and competition among nations for feed supply and demand increased, types of imported feed ingredients, feed importing counties and agr

A possibility of mycotoxin contamination in feed production, storage and transportation has increased due to recent climate change. Since international grain prices and competition among nations for feed supply and demand increased, types of imported feed ingredients, feed importing counties and agrifood by-products also have changed. Therefore, safety management for mycotoxin in feeds has become increasingly important. Mycotoxin contamination in feeds can lead to decreased productivity, increased risk of developing diseases and decreased quality of livestock products. In this study, we examined mycotoxin contamination situation and other risk factors in each feed management step (a port of entry, a mixed feed factory, and a livestock farm) and then suggested main management factors. We also established a technology to reduce mycotoxin in feeds with dose-response assessment.
Before survey of mycotoxin contamination, sampling and analysis methods for deoxynivalenol, zearalenone and fumonisin that are not objects of domestic regulation management were established. These methods were used to examine contamination of 5 types of mycotoxin (aflatoxin, ochratoxin A, deoxynivalenol, zearalenone, and fumonisin) in 17 imported feed ingredients. Results indicated that both aflatoxin (10-50 ppb) and ochratoxin A (200 ppb) in all 17 feed ingredients were detected below the permitted standards. On the other hand, deoxynivalenol, zearalenone and fumonisin in chinese DDGS, gluten feed and wheat gluten　were detected above both US and EU permitted standards.
In addition, feed management situation in each feed management step was examined, and main management factors and acceptable standards were suggested. A technology to reduce mycotoxin in feeds with dose-response assessment was established. Both daily gain and feed intake were reduced at the level of 1.75 ppm Deoxynivalenol and 1.00 ppm zearalenone. We also examined effect of aflatoxin and deoxynivalenol contamination on daily gain and feed intake at each level of contamination using meta-analysis and then predicted equations at each level of contamination (feed intake: -24.9 × aflatoxin concentration - 1.7, -5.49 × deoxynivalenol concentration - 0.13; daily gain: -22.7 × aflatoxin concentration - 4.0, -6.49 × deoxynivalenol concentration + 0.93). Additionally, the following studies were conducted: 1) in vitro evaluation of adsorptive capacity for 10 commercial mycotoxin reducing agents, 2) effect of mycotoxin reducing agents at each level of mycotoxin contamination on productivity and health of broilers, 3) determination of optimum addition levels of mycotoxin reducing agents in feeds, and 4) examination of nutrient availability and establishment of feed ratio model at each addition level mycotoxin reducing agents in swine.
Based on the results of our studies, we held a symposium on ‘Safety management for mycotoxin in feeds and mycotoxin reduction plan’ and produced and disseminated a safety management manual for mycotoxin.