보고서 정보
주관연구기관 |
국립농업과학원 National Institute of Agricultural Sciences |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2014-11 |
과제시작연도 |
2013 |
주관부처 |
농림축산식품부 Ministry of Agriculture, Food and Rural Affairs(MAFRA) |
등록번호 |
TRKO201500011293 |
과제고유번호 |
1545006335 |
사업명 |
생명산업기술개발 |
DB 구축일자 |
2015-07-18
|
DOI |
https://doi.org/10.23000/TRKO201500011293 |
초록
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Ⅳ. 연구개발결과
▪곤충 특유의 좋지 않은 맛과 냄새를 없앤 후 살균, 세척, 동결건조 등 제조공정 확립
▪확립된 제조공정에 의해 제조된 갈색거저리 및 흰점박이꽃무지 유충 분말의 영양성분 분석
: 일반성분, 성상, 아미노산 조성, 지방산 조성, 비타민 및 무기질 조성
▪갈색거저리 및 흰점박이꽃무지의 유해물질 분석 : 세균, 중금속, 잔류농약, 곰팡이독소, 화합물, 알레르기 유발물질
▪갈색거저리 및 흰점박이꽃무지 유충 분말 추출물의 항비만, 항염증, 항당뇨 효능 검정
▪갈색거저리 및 흰점박이꽃무지의 기
Ⅳ. 연구개발결과
▪곤충 특유의 좋지 않은 맛과 냄새를 없앤 후 살균, 세척, 동결건조 등 제조공정 확립
▪확립된 제조공정에 의해 제조된 갈색거저리 및 흰점박이꽃무지 유충 분말의 영양성분 분석
: 일반성분, 성상, 아미노산 조성, 지방산 조성, 비타민 및 무기질 조성
▪갈색거저리 및 흰점박이꽃무지의 유해물질 분석 : 세균, 중금속, 잔류농약, 곰팡이독소, 화합물, 알레르기 유발물질
▪갈색거저리 및 흰점박이꽃무지 유충 분말 추출물의 항비만, 항염증, 항당뇨 효능 검정
▪갈색거저리 및 흰점박이꽃무지의 기원 및 개발경위, 국내·외 인정 및 사용현황 등에 관한 자료 작성
▪갈색거저리 및 흰점박이꽃무지의 추출, 세부 분획 및 분석(일반성분, 지방산 정량, 정유성분 등)을 실시함
▪GC 성분분석을 통해 갈색거저리/흰점박이 꽃무지의 대표물질을 올레산으로 선정함
▪갈색거저리/흰점박이 꽃무지 분획물 및 대표지방산의 in vitro 항산화능, BACE1 저해활성 및 Aβ 유도독성으로부터의 신경세포보호효과를 확인함
▪갈색거저리/흰점박이 꽃무지 대표물질인 올레산의 NF-κB 신경전달 체계 관련 항 염증 반응을 통한 알츠하이머성 치매 예방 메커니즘을 규명함
- 갈색거저리와 흰점박이꽃무지 추출물들의 암세포주에 대한 세포독성 및 세포죽음 유도기전을 규명하였고, 발암동물모델에서 항암활성을 확인함
- 곤충추출물의 간질환에 대한 보호효과를 간독성유도 동물실험에서 규명함
▪갈색 거저리와 흰점박이꽃무지 조제시험물질의 균질성, 함량 및 안정성을 분석할 수 있는 분석법을 확립함
▪갈색거저리 및 흰점박이꽃무지의 안전성평가를 위해 동물모델을 이용하여 급성, 4주 및 13주 독성시험을 실시한 결과, 독성이 나타나지 않음
▪갈색거저리 및 흰점박이꽃무지의 유전독성을 평가하기 위해 복귀돌연변이, 소핵, 염색체이상 시험 및 comet assay를 실시한 결과, 모두 음성이거나 독성이 나타나지 않음
▪갈색거저리와 꽃무지 유충에 대한 멸균 온도, 멸균시간 및 동결 온도, 시간, 진공도 등 건조 조건 설정
▪각각의 원재료에 대한 생산 설비 및 작업 조건 표준화에 의한 제조공정 표준화 완료
▪표준화된 제조공정으로 제조된 원료에 대하여 반복실험을 통해 성상, 이물, 수분, 조 단백 함량, 미생물에 대한 자가 규격 설정
▪동결건조유충을 방습성이 우수한 포장 재질의 기밀용기에 포장하여 가속실험 기간동안 지표물질의 변화를 관찰하고 아레니우스 방정식에 의한 유통기한 설정
▪제품특성을 고려한 디자인제작 및 분말형태의 제형인 시제품 제조
▪시제품에 대한 가속실험으로 자가 규격항목 및 지표물질의 변화를 관찰하고 유통기한 설정
▪동결건조 갈색거저리 및 흰점박이꽃무지 유충에 대한 한시적 인정 요청서 작성
▪동결건조 갈색거저리 유충 새로운 식품원료로 한시적 인정(‘14. 7. 15.)
▪동결건조 흰점박이꽃무지 유충 새로운 식품원료로 한시적 인정(‘14. 9. 30.)
Abstract
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In this study, we established optimal powder manufacturing process for Protaetia brevitarsis and Tenebrio molitor larva for using as a novel food. For this purpose, it should be feed with the oak sawdust sterilized by water vapor for 3-5 days, starved without water or food for 3 days, and then the l
In this study, we established optimal powder manufacturing process for Protaetia brevitarsis and Tenebrio molitor larva for using as a novel food. For this purpose, it should be feed with the oak sawdust sterilized by water vapor for 3-5 days, starved without water or food for 3 days, and then the larvae were sterilized before freeze-drying. The sterilized P. brevitarsis and T. molitor was lyophilzed and grinded by a blender. A safety of the powder as a food was validated by evaluation of Raw 264.7 macrophage cytotoxicity using MTS assay. As above results, we propose that optimal powder manufacturing process established in this study can be used in industrial production of P. brevitarsis and T. molitor as a novel food.
To evaluate P. brevitarsis and T. molitor as food materials, we investigated their composition of nutritional and harmful components. Analysis of nutritional composition (moisture, crude protein, crude fat, crude ash, crude fiber and carbohydrates) showed that the content of crude protein, fat, carbohydrate were by 58%, 18%, 8% in Protaetia brevitarsis powder, and 53%, 31%, 9% in T. molitor powder, respectively. We also confirmed that all bacteria as well as all heavy metals analyzed in this study were not detected in lyophilized T. molitor. In P. brevitarsis, all bacteria were not detected, but small amount of Hg (0.1±0.042 mg/kg) and Pb (0.70±0.49 mg/kg) were detected. And, we also investigated whether their extract have anti-inflammatory, anti-obesity, or anti-diabetic effect on various cell lines and mice.
Alzheimer’s disease (AD) is an progressive neurodegenerative disorder characterized by neuronal loss and extracellular senile plaques containing β-amyloid peptide (Aβ). Aβ is proteolytically produced by β- and γ-secretase from a single transmembrane amyloid precursor protein (APP). The progressive accumulation of insoluble Aβ peptide is considered to initiate a pathogenic cascade of AD. Considerable evidence has been suggesting that the reactive oxygen species (ROS) are involved in the apoptotic mechanism and inflammatory response of Aβ-mediated neurotoxicity. The present study was designed to demonstrate the anti-AD effect of both T. molitor and P. brevitarsis larvae extracts and their major compounds through the regulation of in vitro BACE1, Aβ-induced neurotoxicity and Aβ-induced inflammatory response. The isolated major compounds showed novel neuroprotective properties not only via the selective and specific inhibition of BACE activity but also through the alleviation of Aβ25-35-induced toxicity. Moreover, the results implied that extracts and their isolated compounds attenuated neuroinflammatory responses through the inactivation of NF-κB by NF-κB dependent inflammatory pathways and phosphorylation of MAPK in Aβ25-35-induced PC12 cells.
In vitro and in vivo hepatocellular carcinoma and hepatotoxic mouse models have been analyzed to examine anti-neoplastic and hepato-protective activities of T. molitor and P. brevitarsis larval extracts. The effects of T. molitor and P. brevitarsis larval extracts on cellular signaling of metabolism, growth, and death of hepatocellular carcinoma cells were analyzed by high-throughput screening and high resolution molecular imaging. In vivo hepatocellular carcinoma and hepatotoxic model systems were established and anticancer and hepato-protective activities of the extracts were examined. The extracts showed strong cytotoxicity against hepatocellular carcinoma cells in vitro through the mechanism of apoptosis and autophagy and the antitumor activity was confirmed in vivo mouse xenograft model. Toxic chemical induced hepatotoxic mouse model system was established, where the extract showed strong hepatoprotective activities.
To Evaluate pharmacological action and toxicity of insect resources for development as food and medicinal material, we assess the safety of the edible insects under the GLP condition followed by the registration of the edible insects as a food source. The safety and toxicity assessment for edible insects will contribute to secure the food source in the future. This research is significant to secure the initiating technology in the relevant filed. We carried out the establishment of validation method for T. molitor and P. brevitarsis, the general toxicity studies for T. molitor and P. brevitarsis, and the genotoxicity studies for T. molitor and P. brevitarsis. As a result, the validation method for the homogeneity, contents and stability analysis of T. molitor and P. brevitarsis was established. In the results of acute, 4 weeks and 13 weeks toxicity studies of T. molitor and P. brevitarsis, there were no toxicity findings in any studies. In the results of Ames, chromosomal aberration, micronucleus and comet assay, there were negative findings or no toxicity finding in any studies. The safety of T. molitor and P. brevitarsis was established and these insects will be available for the food sources.
Freeze-dry condition (time, temperature, vacuum level) and sterilization condition (time, temperature) setting in insect resource (P. brevitarsis, T. molitor) for standardization of production process. Also, setting self standard test (appearance, moisture, foreign substance, crude protein content etc.) with standardization of production process based on the Korea Food Standards Codex. Check self standard test and material change on the acceleration test with regard to insect resource. And expiration date set at by the Arrhenius equation. Also, finished producing a prototype of powder form with package design considering product characteristic and check self standard test and material change on the acceleration test with regard to prototype.
Based on the result above, freeze-dring T. molitor and P. brevitarsis registered as a temporary food on Ministry of Food and Drug Safety and reported their manufacturing on city hall.
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