초록

- 콜라겐의 산업용 생산시스템 개발 및 산업화
콜라겐 생산의 산업화를 위한 공정을 개발하기 위하여 시료의 전처리에서부터 추출, 정제에 이르기까지 모든 공정에 대하여 검토하였다. 전처리 공정은 12시간 단축, 초음파를이용한 콜라겐 추출시간은 1/25로 단축, 수율은 2배 이상 증가하는 기술을 개발하여 ㈜청룡수산에 기술이전 하였다.

Abstract ▼

○ Results
To develop a process for the industrialization of collagen production, it was investigated in the all processes from pre-treatment of the sample up to the extraction and purification. The pre-treatment process by the mixer was removed quickly scales, pigment and fat from the sample, and

○ Results
To develop a process for the industrialization of collagen production, it was investigated in the all processes from pre-treatment of the sample up to the extraction and purification. The pre-treatment process by the mixer was removed quickly scales, pigment and fat from the sample, and the processing time was greatly reduced from the 24 hours of conventional method to 12 hours. In order to confirm the extraction conditions of collagen for industrialization, the collagen extraction using a small (8L) circulating ultrasonic system was examined. It was greatly reduced use amount of acid (1/10) and extraction time (1/25), and the yield was two times to compare with the conventional method. Based on the above results, the layout of the ultrasonic system for industrialization was created. The ultrasonic system for industrialization has created a layout that can produce 20L at one time a scale in 1% collagen solution, Industrial ultrasound systems were manufactured based on the layout. After installed the manufactured industrial ultrasonic system at the production field, the efficiency of collagen production was examined. The results were the same as the result in the above-mentioned small-scale circulation system.

On the other hand, when the extracted collagen was examined for authenticity of collagen through enzyme treatment and amino acid analysis, it was confirmed a typical type I collagen. From the pretreatment stage to the production of collagen, we compared with economical efficiency of collagen production by the conventional method and the industrial ultrasonic system, it was confirmed that the cost of collagen produced by the industrial ultrasonic system can be reduced by more than 95%.

From the above results, it was confirmed that the collagen production by the industrial ultrasonic system is very efficient, and the technology transfer to “Choung Ryong Fisheries Co., Ltd.” is completed. The contents of technology are about pre-treatment of material, extraction technology of collagen and operation of ultrasonic system.

목차 Contents

• 표지 ... 1제 출 문 ... 3보고서 요약서 ... 4요 약 문 ... 5SUMMARY ... 6CONTENTS ... 7목차 ... 8제1장. 연구 개발 과제의 개요 ... 10 1-1. 연구 개발 목적 ... 10 1-2. 연구 개발의 필요성 ... 10 1-3. 연구 개발 범위 ... 11제2장. 국내외 기술 개발 현황 ... 12제3장. 연구 수행 내용 및 결과 ... 15 1. 서론 ... 15 2. 재료 및 방법 ... 15 가. 재료 ... 15 나. 시료의 전처리 ... 15 다. 시료의 전처리 전과 후의 일반성분 ... 16 라. 초음파 처리에 의한 콜라겐 추출 ... 16 마. 콜라겐 추출 수율 ... 17 바. SDS-PAGE에 의한 콜라겐 분석 ... 17 사. 콜라겐의 소화 특성 ... 18 아. 아미노산 분석 ... 18 3. 결과 및 고찰 ... 19 가. 산업화를 위한 콜라겐 추출 적정 조건 확인 ... 19 나. 콜라겐 대량 생산을 위한 산업용 초음파 시스템 개발 ... 23 다. 산업용 추출시스템에 의한 콜라겐 추출 현장 시험 ... 28 라. 경제성 분석 ... 32 마. 콜라겐 생산 시스템의 기술이전 ... 34제4장. 목표 달성도 및 관련 분야 기여도 ... 47제5장. 연구 결과의 활용 계획 ... 48제6장. 연구 과정에서 수집한 해외 과학 기술 정보 ... 48제7장. 연구 개발 결과의 보안 등급 ... 48제8장. 국가과학기술종합정보시스템에 등록한 연구 시설·장비 현황 ... 48제9장. 연구 개발 과제의 대표적 연구 실적 ... 48제10장. 기타 사항 ... 48제11장. 참고 문헌 ... 49끝페이지 ... 51