보고서 정보
주관연구기관 |
국립수산과학원 National Fisheries Research and Development Institute |
연구책임자 |
임상구
|
참여연구자 |
김경덕
,
김진도
,
조용철
,
김광석
,
김성원
,
박영철
,
김응오
,
손맹현
,
강용진
,
이혜영
,
김대중
,
김정대
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2011-07 |
과제시작연도 |
2008 |
주관부처 |
해양수산부 Ministry of Oceans and Fisheries |
등록번호 |
TRKO201700007111 |
과제고유번호 |
1545000666 |
사업명 |
첨단양식기술개발 |
DB 구축일자 |
2017-10-12
|
키워드 |
메기.단백질.지질.영양소 요구량.사료원료.실용배합사료.Catfish.Silurus asotus.Protein.Lipid.Nutrient requirements.Feed ingredients.Practical feed.
|
DOI |
https://doi.org/10.23000/TRKO201700007111 |
초록
▼
본 연구에서는 메기용 실용배합사료 개발을 위하여 영양소 요구량 연구, 사료원료 이용성 연구 및 실용 배합사료 평가연구와 관련된 실험사료 제조 및 메기 사육실험과 어체 성분분석을 실시하였다.
1. 메기의 영양소 요구량 연구
- 메기의 단백질 요구량 조사
- 사료내 적정 단백질 및 지질(에너지) 함량 조사
- 메기의 필수지방산 요구
2. 사료원료 이용성 연구
- 메기 사료의 어분 대체 단백질원으로 대두박 단독 첨가 및 대두박과 콘글루텐밀을 혼합 첨가하여 그 이용성을 조사
- 사료내 비
본 연구에서는 메기용 실용배합사료 개발을 위하여 영양소 요구량 연구, 사료원료 이용성 연구 및 실용 배합사료 평가연구와 관련된 실험사료 제조 및 메기 사육실험과 어체 성분분석을 실시하였다.
1. 메기의 영양소 요구량 연구
- 메기의 단백질 요구량 조사
- 사료내 적정 단백질 및 지질(에너지) 함량 조사
- 메기의 필수지방산 요구
2. 사료원료 이용성 연구
- 메기 사료의 어분 대체 단백질원으로 대두박 단독 첨가 및 대두박과 콘글루텐밀을 혼합 첨가하여 그 이용성을 조사
- 사료내 비단백질 에너지원으로써 지질 및 탄수화물 이용성 평가
3. 실용배합사료 개발연구
- 메기용 실험배합사료 및 시판사료의 사육효능 효과실험을 실시하였으며, 소형수조 및 노지 대형수조에서 실용배합사료 효과실험을 실시
(출처 : 보고서 요약 3p)
Abstract
▼
Ⅲ. Results
1. Study on nutrient requirements of catfish
1. 1. Study on protein requirements of catfish
This study was conducted to investigate the optimum protein requirement of far eastern catfish (Silurus asotus). Five diet containing 35, 40, 45, 50 and 55% protein were prepared. Triplica
Ⅲ. Results
1. Study on nutrient requirements of catfish
1. 1. Study on protein requirements of catfish
This study was conducted to investigate the optimum protein requirement of far eastern catfish (Silurus asotus). Five diet containing 35, 40, 45, 50 and 55% protein were prepared. Triplicate groups of fish (initial body weight of 44 g) were hand-fed to apparent satiation for 5 weeks. Mean water temperature was 25.8±1.0℃ during the feeding trial. At the end of the feeding trial, survival was not significantly different among all groups. Final mean weight and weight gain of fish fed the 55% diet was significantly higher than those of fish fed the 35% and 40% diets but were not significantly differ from those of fish fed the 45% and 50% diets. Fish fed the 50% and 55% diets showed significantly higher feed efficiency compared with other diets. Protein efficiency ratio of fish fed the 35% and 40% diet were significantly higher than those of fish fed the 50% and 55% diets but was not significantly differ from that of fish fed the 45% diet. Crude lipid content of the whole body that fish fed the 50% diet was significantly higher than that of fish fed the 55% diet but were not significantly differ from those of fish fed the 35%, 40% and 45% diets. The contents of crude protein and ash in the whole body were not significantly affected by dietary protein contents. The results of this study suggest that optimum dietary protein level would be 45% for growth of juvenile far eastern catfish.
1. 2. Dietary optimum protein and lipid levels for catfish
This study was conducted to investigate the proper dietary protein and lipid levels for growth of catfish (Silurus asotus). Six diets were formulated to contain three levels of protein (20%, 30% and 40%) and two levels of lipid (9% and 17%). Triplicate groups of fish (initial body weight of 7.6 g) were hand-fed to apparent satiation for 5 weeks. Mean water temperature was 24.9±1.6℃ during the feeding trial. Final mean weight was improved with increasing dietary protein and lipid levels, and the highest final mean weight was observed in fish fed the 40/17 (% protein/% lipid) diet. No significant difference was observed in final mean weight for fish fed between 30/17 diet and 40/9 diet. Feed efficiency was improved with increasing dietary protein up to 40% and 30% protein with 9% and 17% lipid, respectively. Feed efficiency of fish fed the 30/17 diet was not significantly different from that of fish fed the 40/9 diet or 40/17 diet. Daily feed intake of fish tended to decrease with increasing dietary protein and lipid levels. Moisture content of whole body in fish fed the 9% lipid diets were significantly higher than that of fish fed the 17% lipid diets at the same protein level, but the opposite trends were found for crude lipid content. Significant effects of dietary lipid were observed for most fatty acids, according to their relative values in the diets. The results of this study suggest that an increase of dietary lipid level from 9% to 17% can improve growth and protein utilization, and the diet containing 40% protein and 17% lipid would be suitable for optimal growth and effective protein utilization of juvenile catfish.
1. 3. Study on essential fatty acid requirements of catfish
This study was conducted to investigate the effects of dietary lipid sources on growth performance and body composition of catfish (Silurus asotus). Three replicate groups of fish (average weighing 3.6 g) were fed experimental diets containing 10% beef tallow (BT), 5% BT plus 5% corn oil (CO), 5% BT plus 5% linseed oil (LO), and 5% BT plus 5% squid liver oil (SO) as the lipid sources for 5 weeks. Mean water temperature was 23.8±1.5℃ during the feeding trial. No significant difference was observed in survival among all groups. Weight gain of fish fed LO and SO diets was significantly higher than that of fish fed CO and BT diets. Feed efficiency of fish fed LO and SO diets was significantly higher than that of fish fed BT diet, but was not significantly different from that of CO diet. Protein efficiency ratio of fish fed SO diet was significantly higher than that of fish fed CO and BT diets, but was not significantly different from that of LO diet. The 18:1n-9 of whole body polar lipid fraction in fish fed beef tallow diet increase compare to that of other groups. Fish fed the CO and LO diets were showed the highest contents of 18:2n-6 and 20:4n-6, and 18:3n-3 in both lipid fractions, respectively. Significantly higher content of n-3 HUFA was observed in both lipid fractions of the liver from fish fed the SO diet than those of fish fed the other diets. The results of this study indicated that linseed oil and squid liver oil containing n-3 fatty acids are good dietary lipid sources for growth of catfish.
2. Studies on availability of feed ingredients
2. 1. Evaluation of soybean meal as a partial substitute for fish meal in diet for catfish
A feeding trials were conducted to evaluate the soybean meal as a substitute for the fish meal in diet for growth of catfish (Silurus asotus). Three replicate groups of juveniles (average weight 7.2g) were fed one of diets containing 0, 10 and 20% soybean meals for 66 days. Mean water temperature was 25±1.7℃ during the feeding trial. Survival and final mean weight were not significantly affected by dietary soybean meal levels. No significant differences were observed in feed efficiency, protein efficiency ratio and protein retention of fish fed between 0% (control diet) and 20% soybean meal diets. However, fish fed 10% soybean meal diet showed the lower feed efficiency, protein efficiency ratio and protein retention, and higher daily feed intake compared with fish fed control diet and 20% soybean meal diet. The contents of moisture, crude protein, crude lipid and ash in the whole body were not significantly affected by dietary soybean meal levels. The results of this study suggest that soybean meal can be used as a substitute for fish meal up to 20% in diet.
2. 2. Evaluation availabilities of soybean meal and corn gluten meal as substitute for fish meal in diet for catfish
This study was conducted to evaluate availabilities of soybean meal and corn gluten meal as substitute for fish meal in diet for catfish. Five diets with different soybean meal and corn gluten meal levels (0, 25, 50, 75 and 88% designated as D1, D2, D3, D4 and D5) were prepared.
Triplicate groups of fish (initial body weight of 93 g) were hand-fed to apparent satiation for 13 weeks. Mean water temperature was 23±0.5℃ during the feeding trial. At the end of the feeding trial, survival was not significantly different among all groups. Final mean weight, feed efficiency and protein efficiency ratio of fish fed D1, D2 and D3 diets were significantly higher than those of fish fed D4 and D5 diets. Daily feed intake of fish fed the D1 diet was significantly higher than those of fish fed the D4 and D5 diets but were not significantly differ from those of fish fed the D2 and D3 diets. The contents of moisture, crude lipid and ash in the whole body were not significantly affected by different soybean meal and corn gluten meal contents. Crude lipid content of the whole body that fish fed the D1 was significantly higher than that of fish fed the D4 and D5 diets but were not significantly differ from those of fish fed the D2 and D3 diets. The results of this study suggest that mixture of soybean meal and corn gluten meal can be used as substitute for fish meal up to 50%.
2. 3. Evaluation availabilities of lipid and carbohydrate in diet of catfish
This study was conducted to investigate the effects of dietary lipid and carbohydrate on growth performance and body composition of catfish (Silurus asotus). Two diets (designated as L9 and L17) with different high lipid and high carbohydrate contents, respectively. Triplicate groups of fish (initial body weight of 3.6 g) were hand-fed to apparent satiation for 6 weeks. Mean water temperature was 24±0.5℃ during the feeding trial. At the end of the feeding trial, survival was not significantly different among dietary treatments. Final mean weight of fish fed high lipid content (L17) diet was significantly higher than that of fish fed high carbohydrate content (L9) diet. Crude lipid content in whole body of fish fed the L17 diet was significantly higher than that of fish fed the L9 diet. The results of this study suggest that 17% lipid diet can improve in growth of juvenile catfish compared with 9% lipid diet.
3. Development of practical feed for catfish
3. 1. Evaluation of experimental practical diet for catfish
A feeding trials were conducted to evaluate experimental practical diet and commercial diet for growth of catfish (Silurus asotus). Three replicate groups of juveniles (average weight 7.3 g) were fed either the formulated diet or commercial diet for 66 days. Mean water temperature was 25±1.7℃ during the feeding trial. Survival and final mean weight were not significantly different among treatment. Significantly higher feed efficiency, protein efficiency ratio and protein retention, and lower daily feed intake were observed in fish fed experimental practical diet compared with fish fed commercial diet. Proximate compositions of the whole body were not significantly affected by diets.
The results of this study suggest that dietary formulation used in experiment diet could be applied to the practical feed for far eastern catfish.
3. 2. Evaluation availabilities of experimental practical diet for catfish in pilot scale
This study was conducted to evaluate three experimental practical diets containing different contents of tuna meal, soybean meal and squid liver oil (designated as EP1, EP2 and EP3) and commercial diet (EP4) for growth of catfish in pilot scale tanks. Triplicate groups of fish (initial body weight of 16 g) were hand-fed to apparent satiation for 19 weeks. Mean water temperature was 25.8±1.2℃ during the feeding trial. At the end of the feeding trial, survival, final mean weight of fish fed EP1 and EP4 diets were significantly higher than those of fish fed EP2 and EP3 diets. Highest Feed efficiency was observed in fish fed the EP4 diet. Proximate compositions of the whole body were not significantly affected by diets. The results of this study indicated that dietary formulation used in EP1 could be applied to the practical feed for catfish.
3. 3. Evaluation availabilities of experimental practical diet for catfish in large sized tanks
This study was conducted to evaluate three experimental practical diets containing different contents of tuna meal, soybean meal and squid liver oil (designated as EP1, EP2 and EP3) and commercial diet (EP4) for growth of catfish in large sized tanks. Fish (initial body weight of 14 g) were hand-fed to apparent satiation for 19 weeks. Mean water temperature was 22.6±1.7℃ during the feeding trial. After feeding trial, similar results were observed in pilot scale experiment. Proximate compositions of the whole body were not significantly different among all groups. The results of this study indicated that dietary formulation used in EP1 could be applied to the practical feed for catfish.
(출처 : SUMMARY 10p)
목차 Contents
- 표지 ... 1
- 제출문 ... 2
- 보고서 요약 ... 3
- 요약문 ... 4
- SUMMARY ... 10
- 목차 ... 15
- CONTENTS ... 16
- 표목차 ... 17
- TABLE LISTS ... 18
- 제 1 장 연구개발과제의 개요 ... 20
- 제 2 장 국내외 기술개발 현황 ... 20
- 제 3 장 연구개발 수행 내용 및 결과 ... 22
- 1. 영양소 요구량 연구 ... 22
- 2. 사료원료 이용성 연구 ... 38
- 3. 실용배합사료 개발 연구 ... 49
- 제 4 장 목표달성도 및 관련분야에의 기여도 ... 58
- 제 5 장 연구개발결과의 활용계획 ... 59
- 제 6 장 참고문헌 ... 60
- 끝페이지 ... 64
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