초록 ▼

• Microcapsulation 최적조건 확립
- 5% 난황항체, 1.0% alginate, 100 mM CaCl2, 1% Chitosan 농도값이 최적 조건인 것으로 확인하였으며, 최적 조건에서 제조된 난황항체 함유 캡슐의 포집 효율은 약 90%임

• microencapsulelation을 이용한 난황항체 함유 캡슐 제조
- 키토산, 알지네이트 등의 천연 다당류를 이용하여 이온결합을 이용한 캡슐화 방법을 통해 난황항체를 함유한 캡슐을 제조하여 제조조건에 따른 입자특성을 연구하였음.

- 피복물질

• Microcapsulation 최적조건 확립
- 5% 난황항체, 1.0% alginate, 100 mM CaCl2, 1% Chitosan 농도값이 최적 조건인 것으로 확인하였으며, 최적 조건에서 제조된 난황항체 함유 캡슐의 포집 효율은 약 90%임

• microencapsulelation을 이용한 난황항체 함유 캡슐 제조
- 키토산, 알지네이트 등의 천연 다당류를 이용하여 이온결합을 이용한 캡슐화 방법을 통해 난황항체를 함유한 캡슐을 제조하여 제조조건에 따른 입자특성을 연구하였음.

- 피복물질인 알지네이트의 농도, 알지네이트와 ionic gelation을 유발하는 칼슘이온의 농도, 경화시간에 따른 포집효율 및 방출패턴의 유의적인 영향을 확인함

• 특허출원 - 특이 난황항체를 함유하는 키토산 -알지네이트 캡슐의 생산방법

• 시제품 제작 - Helicobacter pylori에 대한 특이 난황항체를 마이크로캡슐화하여 시제품을 제작함

(출처 : 요약서 3p)

Abstract ▼

Purpose&Contents
1. Purpose of the Study Development
The development of stability-improved egg yolk by microencapsulation and the development of food beneficial on the intestine condition and body weight control by using the microcapsules containing egg yolk

2. Content of the study
1

Purpose&Contents
1. Purpose of the Study Development
The development of stability-improved egg yolk by microencapsulation and the development of food beneficial on the intestine condition and body weight control by using the microcapsules containing egg yolk

2. Content of the study
1) Extraction of the egg yolk and Determine of the Process
Through the using of immunized egg of hen immunized by injection, egg yolk powder containing high purified IgY (Anti Lipase IgY, Anti Coli IgY, Anti Sal IgY, Anti Helico IgY) was produced.

2)Determine of the microencapsulation process of igY and its evaluation
For the use of coating materials, the edible and high encapsulation efficiency-having sodium alginate was selected, and as the second coating material, chitosan was chosen.

3) Standardization of egg yolk as thr raw material
By using SEM, the size and surface consistency of egg yolk was confirmed and through BCA assay and ELISA, it was also confirmed that the egg yolk was maintaing its activity in the intestinal environment

4) Manufacturing of the test product
Through the mass production of microencapsulated igY, not only food for human but also medical therapy products for animal were developed.

Results
• The problematic low availability of the oral passive immunization could be overcame. Thus, it is expected that the present market of egg yolk antibody is activated.

• By mass production of the high purified egg yolk with low cost and development of stabilization, it became possible that this present product can be differentiated from the other similar products and have an more fortified market competitive ability, in the field of various functional foods and animal medical products.

Through this study performance, the unstability of the existing present egg yolk antibody products can be highly improved and high qualified food production could be possible.

Expected Contribution
Products using microencapsulation techniques still are not in the market, if this study can be developed successfully, a new exploitation could be possible into not only domestic but also international market. For the more, there are not many egg yolk antibody companies in the world, especially it is a blue ocean market without any special leading global company. Thus, it is expected that an positive propulsion can promise an global growing company.

〇 By exploitation of an mass production technique of egg yolk antibody with low cost and high purified stability, this new product can be differentiated from the other existing products have more fortified competitive ability.

〇 Recently, due to FT As with USA, Canada etc, and at the present status of the decreasing income of the agricultural houshold, the government has been supplementing their deficits and recommending more beneficial agricultural items. Compared to the general eggs, the immunized eggs including special antibodies are expected to improve the income of the hen-feeding agricultural household.


(출처 : SUMMARY 9p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 3
• 보고서 요약서 ... 5
• 요 약 문 ... 7
• SUMMARY ... 9
• CONTENTS ... 11
• 목차 ... 12
• 1. 연구개발과제의 개요 ... 13
• 제 1절. 연구개발 목적 ... 13
• 제 2절. 연구개발의 필요성 ... 13
• 제 3절. 연구개발 범위 ... 16
• 2. 국내외 기술개발 현황 ... 18
• 3. 연구수행 내용 및 결과 ... 19
• 제 1절. 연구개발의 배경 및 수행내용 ... 19
• 제 2절. 연구내용 및 결과 ... 20
• 제 3절. 연구성과 결과 및 고찰 ... 40
• 4. 목표달성도 및 관련분야 기여도 ... 46
• 5. 연구결과의 활용계획 ... 48
• 6. 연구과정에서 수집한 해외과학기술정보 ... 49
• 7. 연구개발결과의 보안등급 ... 50
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 51
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 52
• 10. 연구개발과제의 대표적 연구실적 ... 53
• 11. 기타사항 ... 54
• 12. 참고문헌 ... 55
• 별첨 1 연구개발보고서 초록 ... 57
• 끝페이지 ... 60