초록

- 델로비브리오 박테리오보루스(포식균)를 이용한 무항생제 치료법 개발 가능성에 대한 연구를 진행
- 델로비브리오 박테리오보루스(포식균)의 치료물질로서의 한계점과 불안정성 및 그에 대한 대안점을 확인함으로서 후속연구에 대한 기반연구 진행

( 출처: 보고서 요약서 3p )

Abstract ▼

□ Purpose&Contents
□ Evaluation of the predatory bacterial toxicity and their possibility to apply real treatment before the clinical trial
○ In In-vivo model, evaluation the predation against the pathogens which could make pneumoniae
○ Develope the systemic treatment process using the Bdel

□ Purpose&Contents
□ Evaluation of the predatory bacterial toxicity and their possibility to apply real treatment before the clinical trial
○ In In-vivo model, evaluation the predation against the pathogens which could make pneumoniae
○ Develope the systemic treatment process using the Bdellovibrio bacteriovorus HD100 with other chemicals which block the biofilm

□ Results
□ For pre-clinical treatment, evaluation of potential toxicity of predators against host cells and hosts
- Against Nuli-1 cell & T84, different epithelial cells were evaluated with predators and their results were reported at “Scientific reports” (IF : 5.64)
- Based upon MTT assay, there are no ciritical damage to host by predatory bacteria
- Based upon cytokine evaluation with predators in in-vitro test, there are no difference with buffer which means not toxic to host
- Identification of anti-biofilm agents and usage
- As research proceeded, supporting materials from natural microbial interactions might give aids to predatory bacteria, and supporting the shortage of predation treatment.
- We defined there are specific relationship between the pathogens (A. baumannii and S. aureus) (Biofouling에 제출 후 Review 중임)
- Natural compounds violacein could remove the gram positive strains which could not be treated with predatory bacteria (Scientific report에 보고, IF : 5.64)
- Evaluation of predatory bacterial physiological properties and their potential attack on gram positive bacterial biofilms
- The findings are submitted to ISME (IF : 9.6, Top 10%) under review

□ Expected Contribution
In this research, we figured out the physiological property of predatory bacteria and their potential usage as treatment for multidrug resistant bacteria. Especially, gram negative pathogens suggested 3 log reduction in their biofilms and their viability. Moreover this research suggest this predatory bacteria could use as clear treatment without any of potential harms against the host and even little positive impacts on in-vivo model. And then we found several potential problems with predatory bacteria and trouble shooting methods

( 출처: 영문 요약문 5p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• 영문 요약문 ... 5
• 영문목차 ... 6
• 목차 ... 7
• 1. 연구개발과제의 개요 ... 8
• 1-1 연구개발 목적 ... 8
• 1-2. 연구개발의 필요성 ... 8
• 2. 국내외 기술개발 현황 ... 11
• 3. 연구수행 내용 및 결과 ... 12
• 4. 목표달성도 및 관련분야 기여도 ... 37
• 4.1 목표달성도 ... 37
• 5. 연구결과의 활용계획 ... 39
• 6. 연구과정에서 수집한 해외과학기술정보 ... 41
• 7. 연구개발성과의 보안등급 ... 42
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 43
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 44
• 10. 연구개발과제의 대표적 연구실적 ... 45
• 11. 기타사항 ... 46
• 12. 참고문헌 ... 46
• [별첨4] 실적 증빙자료 ... 47
• 끝페이지 ... 53