보고서 정보
주관연구기관 |
국립농업과학원 National Institute of Agricultural Sciences |
연구책임자 |
김기창
|
참여연구자 |
김진숙
,
김상범
,
김경미
,
최송이
,
유아미
,
장미
,
박미정
,
조인재
|
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2018-02 |
과제시작연도 |
2017 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
사업 관리 기관 |
농촌진흥청 Rural Development Administration |
등록번호 |
TRKO201800043201 |
과제고유번호 |
1395049837 |
사업명 |
농업기초기반연구 |
DB 구축일자 |
2018-12-15
|
키워드 |
식용꽃.피부건강.항염증.항비만.edible flower.obesity.inflammation.anti-wrinkle.
|
DOI |
https://doi.org/10.23000/TRKO201800043201 |
초록
본 연구에서는 농가에서 재배되며 식용으로 허용이 가능한(식품원 재료DB, 식약처) 다소비 식용꽃 5종 (한련화, 금어초, 팬지, 비올라, 쥬리안) 을 소재로 다양한 생리활성 평가를 실시하였다. 항비만과 피부건강 개선효과를 조사하기 위하여 3T3-L1, CCD 986sk 세포주를 이용하여 관련 bio-marker를 설정하고 분석하였다.
(출처 : 보고서 요약서 4p)
Abstract
▼
Purpose&Contents
Edible flowers are increasingly used in food preparations, they are widely used in food preparations. They has been used for disinfectant, wound -healing, and antibiotics as herbal medicine. But, anti-obesity, anti-inflammation and effect of edible flower has not been reported. T
Purpose&Contents
Edible flowers are increasingly used in food preparations, they are widely used in food preparations. They has been used for disinfectant, wound -healing, and antibiotics as herbal medicine. But, anti-obesity, anti-inflammation and effect of edible flower has not been reported. Therefore, the present study aimed to identify the functionality of edible flower to explore whether it can be used as a basic ingredient in the development of agents for anti-obesity, improving skin-health and preventing inflammatory diseases.
Results
As the adipogenesis plays critical role in obesity, this study was performed to investigate the anti-adipogenic effect of T. majus ethanolic extract (TME) on 3T3-L1 cells. As a result of Oil-Red O staining and triglyceride contents assay, the most inhibition of lipid accumulation was observed at the concentration of 20~500 μg/ml TME. Also, TME concentrations ranging from 20 μg/ml to 500 μg/ml dose-dependently decreased expression of adipocyte differentiation regulator; peroxisome proliferator-activated receptor γ (PPARγ), CCAAT element binging protein α (C/EBPα), and sterol regulatory element binding protein 1 (SREBP-1). According to this study, TME inhibited lipid accumulation and decreased expression of proteins; PPARγ, C/EBPα, and SREBP-1 associated with adipocyte differentiation in 3T3-L1 cells. Also, we investigated the antioxidative properties and anti-inflammatory effects of AM extract (AME) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. AME showed high radical-scavenging ability.
Viability of RAW 264.7 cells was not significantly altered by AME at the concentrations of 0–300 μg/mL. LPS-induced nitric oxide (NO) production was decreased by treatment with 0 –300 μg/mL AME in a concentration-dependent manner. AME pretreatment significantly inhibited the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a concentration-dependent manner. AME also considerably inhibited the mRNA and protein expression of inflammatory cytokines, such as tumor necrosis factor-a (TNF-α), interleukin-1 β (IL-1β), and interleukin-6 (IL-6). Finally, in order to investigate the bioactivity of extract from AM for anti-wrinkle, we measured polyphenol & flavonoids contents, its free radical scavenging activity, expression of MMP-1 (matrix metalloprotease-1), and pro-collagen type-1(PIP) in normal human fibroblast cells (CCD986sk). To isolate the main components from the AM, we extracted by 95% EtOH for 24h.
Antirhinum majus ethanol extract (AME) showed the high-antioxidant activities radical scavenging with 84 % (DPPH). AME, at the concentration of 300 ìg/mL, inhibited 91% cell viability, 30% the matrix metalloprotease (MMP)-1 and increased 55% the pro-collagen type-1(PIP). In addition, the results from Western blot assay showed that this fraction (300 ìg/mL) expressed the MMP-1 protein level by decreasing 43%.
Expected Contribution
The results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. As result, TME may be a potential novel therapeutic agent for the prevention and treatment of obesity. Also, the findings of our study may serve as important basic data for studies on anti-inflammatory substances via extraction of active ingredients from AM, as well as studies on isolation and mechanism of action of inflammation-inhibiting components for prevention or treatment purposes.
(출처 : Summary 6p)
목차 Contents
- 표지 ... 1
- 제 출 문 ... 3
- 보고서 요약서 ... 4
- 국 문 요 약 문 ... 5
- Summary ... 6
- 목차 ... 8
- 제 1 장 연구 개발 과제의 개요 ... 9
- 제1절 연구 개발 목적 ... 9
- 제2절 연구 개발의 필요성 ... 9
- 제3절 연구 개발 범위 ... 10
- 제 2 장 국내외 기술개발 현황 ... 10
- 제 3 장 연구 수행 내용 및 결과 ... 11
- 제 4 장 목표달성도 및 관련분야 기여도 ... 42
- 제1절 : 목표대비 달성도 ... 42
- 제2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 42
- 제 5 장 연구 결과의 활용 계획 ... 42
- 제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 43
- 제 7 장 연구 개발 결과의 보안 등급 ... 43
- 제 8 장 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 43
- 제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 43
- 제 10 장 연구개발과제의 대표적 연구실적 ... 44
- 제 11 장 참고문헌 ... 45
- 끝페이지 ... 50
※ AI-Helper는 부적절한 답변을 할 수 있습니다.