[보고서]메이신 고함유 옥수수수염 추출물의 비임상효능 스크리닝 및 기전구명을 통한 기능성 제품실용화

김우경

메이신 고함유 옥수수수염 추출물의 비임상효능 스크리닝 및 기전구명을 통한 기능성 제품실용화
Practical application of high concentration maysin corn silk extract through the investigation of biological efficacy and mechanism 원문보기

보고서 정보
주관연구기관	단국대학교 DanKook University
연구책임자	김우경
참여연구자	하애화 , 김소라 , 최현지 , 김명환 , 강현중 , 이상섭 , 박유진 , 유승현 , 한재은 , 이미자 , 서우덕 , 최식원 , 정건호 , 김선림
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2018-01
과제시작연도	2017
주관부처	농촌진흥청 Rural Development Administration(RDA)
등록번호	TRKO201800043480
과제고유번호	1395050208
사업명	농축산물부가가치향상
DB 구축일자	2019-05-18
키워드	옥수수수염.메이신.비임상효능.안전성.기능성제품.corn silk.maysin.biological efficacy.safety.functional product.
DOI	https://doi.org/10.23000/TRKO201800043480

초록 ▼

본 연구과제는 메이신을 다량 함유하고 있는 옥수수 수염 추출물의 다량 분리법을 개발하고, 비임상 효능 스크리닝 및 기전을 규명하며, 추출물의 제제연구 및 기능성 제품 개발하여 메이신 고함유 옥수수수염 추출물을 고부가가치 산업소재화 하는 것을 목적으로 함. 세부별 연구성과는 다음과 같음. 1세부: 옥수수수염 함유 메이신 등 활성물질 변화 최소화 기술이 개발되었고, 기능성 천연색소로서 식품/비식품 소재적용을 위한 안정성을 평가하고, 분취용 컬럼을 이용한 maysin 등 유사물질 다량분리법 확립하고, 옥수수수염 추출물의 피부보호 효능 활성 및 소재적용 하여 제품을 개발하고, 옥수수수염 추출물의 항당뇨 효능을 확인함. 2세부: 옥수수수염 추출물의 항비만개선, 전립선 질환 개선, 항산화 효능을 통한 인지능력 개선 비임상 효능 검정 및 기전이 규명되고, 옥수수수염 추출물의 in vivo 안전성을 평가하였음. 3세부: 표준화된 원료의 다량 추출공정 확립을 통한 원료 및 최적 제조공정을 표준화하고, 비임상 효능이 입증된 원료를 이용한 새로운 제형(미세캡슐) 제조공정을 확보하였고, 기능성이 입증된 메이신 고함유 옥수수수염 농축액을 이용한 기능성 식품 신제품(요구르트, 머핀, 식혜)을 개발하였음.

(출처 : 보고서 요약서 3p)

Abstract ▼

Purpose&Contents
The purpose of this study was to develop a large-scale separation method of corn silk extract containing a high amount of maysin and to investigate the in vivo efficacy screening and their mechanism. The ultimate goal was to make the corn silk extract a high value-added industrial material.

Results
[Part Ⅰ project]
○ Established conditions for minimizing the change of active substance according to corn storage and extraction condition
○ Evaluated color stability of corn silk extract with respect to heat, light, sugar addition, and pH for food/non-food application as a functional natural pigment
○ Established separation method for high maysin containing corn silk extract and a high amount of maysin
○ Determined protection effect of skin cells from UV and moisturizing effectanti-inflammatory activity, and atopy improvement, antidiabetic efficacy of corn silk extract

[Part 2 project]
○ Determined the effect of corn silk extract on body weight and body fat reduction and its related mechanism
○ Determined the effect of corn silk extract on prostate enlargement and its mechanism
○ Determined the effects of corn silk extract on improvement of cognitive function and its mechanism
○ Safety conformed by single oral toxicity test and 4 weeks DRF test, genetic toxicity test

[Part 3 project]
○ Established standardization condition of raw materials through washing, drying and powder process as extraction pre-treatment process
○ Established standardization conditions of extraction process by optimization of extraction and enzyme treatment process
○ Analyzed the optimization of micro-encapsulation process, processability, and storage stability
○ Developed yogurt, dressing, muffins and Sikhye with corn silk extract containing high maysin

Expected Contribution
○ Applying for farming and transferring technology to farming groups and food producers. (In addition, the holding of business briefing session for interested organizations and businesses or encouraging them to commercialize for corn silk extract)
○ Presenting the results of the research through the publication of papers, national and international conferences, and patent applications.

(출처 : Summary 5p)

목차 Contents

표지 ... 1
제 출 문 ... 2
보고서 요약서 ... 3
국 문 요 약 문 ... 4
Summary ... 5
목차 ... 6
제 1 장 연구 개발 과제의 개요 ... 7
제1절 연구 개발 목적 ... 7
제2절 연구 개발의 필요성 ... 8
제3절 연구 개발 범위 ... 12
제 2 장 국내외 기술개발 현황 ... 13
제1절 국·내외 관련분야에 대한 기술개발현황 ... 13
제2절 본 연구결과가 국·내외 기술개발현황에서 차지하는 위치 ... 19
제 3 장 연구 수행 내용 및 결과 ... 21
제1절 세부과제 1 ... 21
제2절 세부과제 2 ... 58
제3절 세부과제 3 ... 137
제 4 장 목표달성도 및 관련분야 기여도 ... 252
제1절 : 목표대비 달성도 ... 252
제2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 253
제 5 장 연구 결과의 활용 계획 ... 254
제1절 연구결과의 활용방안 ... 254
제2절 기대성과 및 파급효과 ... 254
제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 255
제 7 장 연구 개발 결과의 보안 등급 ... 258
제 8 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 258
제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 259
제1절 연구실 안전 점검 체계 및 실시 ... 259
제2절 교육 훈련 ... 259
제3절 보험 가입 현황 ... 260
제4절 연구활동종사자 건강검진 실시 ... 260
제5절 추가이행 ... 260
제 10 장 연구개발과제의 대표적 연구실적 ... 261
제 11 장 기타사항 ... 262
제 12 장 참고문헌 ... 263
끝페이지 ... 265

표/그림 (247)

표 옥수수수염 추출물의 타겟 생리활성에 대해 식약처에서 인정한 원료 또는 성분
표 비만(체지방감소)로 인정받은 원료의 예시
표 전립선 건강으로 인정받은 원료의 예시
표 인지능력개선으로 인정받은 원료의 예시
표 옥수수수염 생체 주정 (Prethanol A) 농도별 메이신 함량 변화
표 옥수수수염 동결건조 후 주정(Prethanol A) 농도별 메이신 함량 변화
표 옥수수수염 생체(A) 및 동결건조(B) 주정(Prethanol A) 농도 및 저장 기간별 메이신 함량 변화
표 옥수수수염생체 추출용매별 메이신 함량 변화
표 옥수수수염 채취시기 및 수정에 따른 메이신 함량 변화
표 옥수수수염 추출물의 농도별 광 및 열(온도) 안정성 평가
표 옥수수수염 추출물의 당 및 유기산 농도별 안정성 평가
표 NICS-1의 농도별 광 및 열(온도) 안정성 평가
표 NICS-1의 당 및 유기산 농도별 안정성 평가
표 NICS-3의 농도별 광 및 열(온도) 안정성 평가
표 NICS-3의 당 및 유기산 농도별 안정성 평가
표 세포 독성 확인
표 히알루론산 합성 촉진능 확인
표 옥수수수염 추출물, NICS1과 NICS 3의 항산화 활성
표 NICS-1의 HPLC 크로마토그램 Preparative C18 column chromatography를 이용하여 Fraction 1과 Fraction 2로 분취함
표 Fraction 1을 Recycling prep LC로 재분리하여 얻어진 Fr-1-1의 HPLC 크로마토그램
표 Fraction 2을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1의 HPLC 크로마토그램
표 Fraction 2-1을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1-1의 HPLC 크로마토그램
표 Fraction 2-1을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1-1의 HPLC 크로마토그램
표 NO생성 억제 효능을 통한 항염 효능 평가 (###P <0.005 versus negative control; ***P <0.005 versus positive control)
표 NO생성 억제 효능을 통한 항염 효능 평가
표 TNF-α assay 억제능을 통한 항염 효능 평가 (###P <0.005 versus negative control; ***P <0.005 versus positive control)
표 TNF-α assay 억제능을 통한 항염 효능 평가
표 UV 보호 효능 평가 (###P <0.005 versus negative control; **P <0.01, ***P <0.005 versus positive control)
표 UV 보호 효능 세포 사진 (x200)
표 UV보호 효능 평가
표 세포 증식 효능 평가 (***P <0.005 versus control)
표 세포 증식 효능 평가
표 MIC test를 이용한 시료의 여드름 균 개선 효능 평가
표 MIC test를 이용한 시료의 여드름 균 개선 효능 평가
표 TARC 생성 억제능 시험을 이용한 아토피 개선 효능 평가 (*P<0.05,**P<0.01,***P<0.005)
표 TARC 생성 억제능 시험을 이용한 아토피 개선 효능 평가
표 NICS-1의 HPLC 크로마토그램 Preparative C18 column chromatography를 이용하여 Fraction 1과 Fraction 2로 분취함
표 Fraction 2을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1의 HPLC 크로마토그램
표 Fraction 2-1을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1-1의 HPLC 크로마토그램
표 Fraction 2-1을 Recycling prep LC로 재분리하여 얻어진 Fr-2-1-1의 HPLC 크로마토그램
표 옥수수수염 추출물 및 메이신 α-glucosidase 저해 활성
표 옥수수수염 추출물 및 메이신 α-amylase 저해 활성
표 실험식이조성 (g/kg diet)
표 단회 투여 독성 시험군의 구성
표 4주 DRF 시험군의 구성
표 4주 DRF 시험 뇨검사 항목
표 4주 DRF 시험 장기무게 측정
표 4주 DRF 시험 혈액 생화학적 검사
표 초기체중, 최종체중 및 체중증가
표 실험동물의 체중변화
표 간과 지방조직에서 지방세포의 변화
표 혈액 내 지방성분
표 혈당과 인슐린 농도 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 혈액내 leptin 량(A)과 지방조직내 leptin(B), STAT3(C) 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 혈액내 adiponectin량과 지방조직내 adiponectin 유전자발현
표 지방조직내 TNF-α 유전자발현
표 지방조직내 지방세포분화와 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 간(A, B)과 지방조직(C, D, E)내 지방축적과 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 간내 총지방, 콜레스테롤, 중성지방량 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 간내 지방합성과 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 지방조직내 지방합성과 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 간내 지방분해와 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 지방조직내 지방분해와 관련된 유전자발현 1) LF, low fat diet; HF, high fat diet; HF+NICS1-100, HF+NICS1 100mg/kg; HF+NICS3-100, HF+NICS3 100mg/kg, HF+NICS3-200, HF+NICS1 200mg/kg. * : The significant was determined by T-test between HF and each experimental group (p<0.05)
표 사망률 요약
표 임상증상 요약
표 시험동물의 체중변화
표 부검시 이상소견
표 임상증상 요약
표 4주 DRF 시험동물의 체중변화
표 사료섭취변화
표 물 섭취변화
표 뇨 검사결과
표 혈액생학적 검사
표 장기무게
표 부검소견
표 조직병리학적 검사
표 실험식이
표 복귀 돌연변이시험에 사용한 S9 mixture 구성
표 양성대조물질
표 염색체 이상시험에 사용한 S9 mixture 구성
표 시험군의 구성
표 체중변화
표 장기무게
표 전립선 무게 Group: S, corn oil + ethanol injection(9:1) and distilled water oral intake; T, testosterone injection (10 mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg). #: a>b The different letters indicate significant differences among groups at α=0.05 at determined bu Duncan’s multiple range test
표 전립선 조직의 H T, testosterone injection (10mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg)
표 혈청 AST과 ALT 활성
표 혈청 DHT 농도 Group: S, corn oil + ethanol injection(9:1) and distilled water oral intake; T, testosterone injection (10 mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg). #: a>b The different letters indicate significant differences among groups at α=0.05 at determined bu Duncan’s multiple range test
표 혈청 5α-reductase 2 농도 Group: S, corn oil + ethanol injection(9:1) and distilled water oral intake; T, testosterone injection (10 mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg). #: a>b The different letters indicate significant differences among groups at α=0.05 at determined bu Duncan’s multiple range test
표 전립선 조직 5α-reductase 2 농도 Group: S, corn oil + ethanol injection(9:1) and distilled water oral intake; T, testosterone injection (10 mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg). #: a>b The different letters indicate significant differences among groups at α=0.05 at determined bu Duncan’s multiple range test
표 전립선 조직 PSA 농도 Group: S, corn oil + ethanol injection(9:1) and distilled water oral intake; T, testosterone injection (10 mg/kg) and distilled water oral intake; F, testosterone injection (10 mg/kg) and finasteride intake; N1 10, testosterone injection (10 mg/kg) and NICS1 (10 mg/kg); N1 100, testosterone injection (10 mg/kg) and NICS1 (100 mg/kg). #: a>b The different letters indicate significant differences among groups at α=0.05 at determined bu Duncan’s multiple range test
표 Bacterial reverse mutation assay of with S9 mixture
표 Bacterial reverse mutation assay of without S9 mixture
표 Viable cell counts of chromosome aberration test
표 Summary of results obtained from chromosome aberration test
표 주요 임상증상과 체중변화
표 소핵실험의 주요결과
표 실험에 사용한 유전자의 염기서열
표 4주 DRF 시험군의 구성
표 13주 독성 시험 혈액학적 검사
표 13주 독성 시험 혈액 생화학 검사
표 실험동물의 체중변화, 식이섭취량과 식이효율
표 실험동물의 장기무게
표 옥수수수염 추출물이 인지능력에 미치는 영향-Y-maze 실험 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05).(A) Alternation behavior(%) was calculated as follows Alternation behavior(%)= total number of alteration / (total number of arm entry - 2) x 100. (B) Total number of arm entry under the same experimental condition
표 옥수수수염 추출물이 인지능력에 미치는 영향-수동회피실험 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 acetylcholine 함량에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). Mean±Standard error. The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 acetylcholine 합성효소 mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 acetylcholine esterase 함량에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 acetylcholine esterase mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 acetylcholine esterase 활성에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 무스카린성 아세틸콜린 수용체 M1(A), M2(B) mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 BDNF (Brain derived neurotrophic factor) 함량에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 BDNF (Brain derived neurotrophic factor) mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 SOD 함량에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 환원형 글루타치온 함량에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 NAD(P)H:quinone oxidoreductase 1 (NQO1)의 mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 heme oxygenase 1 (HO-1))의 mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 glutathione S-transferase (GSTs) 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 옥수수수염 추출물이 뇌의 nuclear factor E2-related factor 2(Nrf-2)의 mRNA 발현에 미치는 영향 Control: Control, Sco: Scopolamin (1 mg/kg), ScoTar: Scopolamin (1 mg/kg) + Tarcrine (10 mg/kg), ScoCSE100: Scopolamin (1 mg/kg) + CSE100 (100 mg/kg), ScoCSE200: Scopolamin (1 mg/kg) + CSE200 (200 mg/kg). The significant was determined by duncan’s multiple range test (p<0.05)
표 13주 안전성 실험의 체중. 식이섭취량과 물 섭취량
표 13주 안전성 실험의 장기무게
표 113주 안전성 실험의 혈액학적 분석 결과
표 13주 안전성 실험 혈액의 생화학적 분석 결과
표 옥수수수염과 관련된 국내 판매제품
표 옥수수수염과 관련된 국외 판매제품
표 옥수수수염 추출물의 제조방법
표 옥수수수염 추출물의 시험성적서
표 옥수수수염 추출물의 안전성평가를 위한 의사결정도
표 Factors and levels of experiment for ethanol extraction of corn silk
표 Central composite design for ethanol extraction of corn silk and its dependent variables
표 Factors and levels of experiment for Novozym 33095 enzyme treatment of corn silk extracts
표 Central composite design for Novozym 33095 enzyme treatment of corn silk extracts and its dependent variables
표 Factors and levels of experiment for Pectinex Ultra SP-L enzyme treatment of corn silk extracts
표 Central composite design for Pectinex Ultra SP-L enzyme treatment of corn silk extracts and its dependent variables
표 Factors and levels of experiment for Celluclast 1.5L FG enzyme treatment of corn silk extracts
표 Central composite design for Celluclast 1.5L FG enzyme treatment of corn silk extracts and its dependent variables
표 Instrument and analysis conditions for maysin
표 Change in the number of microorganisms according to washing method of corn silk
표 Appearances of ethanol extracted corn silk. A: Provided sample from RDA, B: Hot air drying at 40℃, C: Far-infrared drying at 40℃
표 Phytochemical contents and bioactivities of corn silk according to drying methods
표 Ethanol extracted maysin contents according to particle size of powdered corn silk
표 Caking degrees of corn silk powders at ambient storage(25℃, 75% RH)
표 Appearances of caking phenomena for corn silk powders at ambient storage(25℃, 75% RH). A: 40 mesh, B: 100 mesh, C: 200 mesh
표 Water absorption of corn silk powders at ambient storage(25℃, 75% RH)
표 Appearance of corn silk extracts
표 Central composite design for ethanol extracts from corn silk and its dependent variables
표 Analysis of variance for the effects of three variables on total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of ethanol extracts from corn silk
표 Regression coefficients of second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of ethanol extracts from corn silk
표 Determination coefficients and probability second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of ethanol extracts from corn silk
표 Contour plot for total polyphenol contents (mg/100g) of ethanol extracts from corn silk (Extraction time, A: 1 hr, B: 3.5 hr, C: 6 hr ; Extraction temperature, D: 20℃, E: 40℃, F: 60℃ ; Ethanol concentration, G: 30%, H: 60%, I: 90%)
표 Contour plot for total flavonoid contents (mg/100g) of ethanol extracts from corn silk (Extraction time, A: 1 hr, B: 3.5 hr, C: 6 hr ; Extraction temperature, D: 20℃, E: 40℃, F: 60℃ ; Ethanol concentration, G: 30%, H: 60%, I: 90%)
표 Contour plot for maysin contents (mg/100g) of ethanol extracts from corn silk (Extraction time, A: 1hr, B: 3.5 hr, C: 6 hr ; Extraction temperature, D: 20℃, E: 40℃, F: 60℃ ; Ethanol concentration, G: 30%, H: 60%, I: 90%)
표 Contour plot for DPPH radical scavenging (%) of ethanol extracts from corn silk (Extraction time, A: 1 hr, B: 3.5 hr, C: 6 hr ; Extraction temperature, D: 20℃, E: 40℃, F: 60℃ ; Ethanol concentration, G: 30%, H: 60%, I: 90%)
표 Contour plot for tyrosinase inhibition (%) of ethanol extracts from corn silk (Extraction time, A: 1 hr, B: 3.5 hr, C: 6 hr ; Extraction temperature, D: 20℃, E: 40℃, F: 60℃ ; Ethanol concentration, G: 30%, H: 60%, I: 90%)
표 Response optimization for mult-response surface of extracts from corn silk
표 Central composite design for Novozym 33095 enzyme treatment from corn silk extract and its dependent variables
표 Analysis of variance for the effects of three variables on total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of ethanol extracts from corn silk
표 Regression coefficients of second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Novozym 33095 enzyme treatments from corn silk extracts
표 Determination coefficients and probability second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Novozym 33095 enzyme treatments from corn silk extracts
표 Contour plot for total polyphenol contents (mg/100g) of Novozym 33095 treatment from corn silk extracts (Reaction time, A: 60 min, B: 90 min, C: 120 min ; Reaction temperature, D: 20℃, E: 40℃, F: 60℃ ; Enzyme concentration, G: 0.05 mL/L, H: 0.15 mL/L, I: 0.25 mL/L)
표 Contour plot for total flavonoid contents (mg/100g) of Novozym 33095 treatment from corn silk extracts (Reaction time, A: 60 min, B: 90 min, C: 120 min ; Reaction temperature, D: 20℃, E: 40℃, F: 60℃ ; Enzyme concentration, G: 0.05 mL/L, H: 0.15 mL/L, I: 0.25 mL/L)
표 Contour plot for maysin contents (mg/100g) of Novozym 33095 treatment from corn silk extracts (Reaction time, A: 60 min, B: 90 min, C: 120 min ; Reaction temperature, D: 20℃, E: 40℃, F: 60℃ ; Enzyme concentration, G: 0.05 mL/L, H: 0.15 mL/L, I: 0.25 mL/L)
표 Contour plot for DPPH radical scavenging activity (%) of Novozym 33095 treatment from corn silk extracts (Reaction time, A: 60 min, B: 90 min, C: 120 min ; Reaction temperature, D: 20℃, E: 40℃, F: 60℃ ; Enzyme concentration, G: 0.05 mL/L, H: 0.15 mL/L, I: 0.25 mL/L)
표 Contour plot for tyrosinase inhibition (%) of Novozym 33095 treatment from corn silk extracts (Reaction time, A: 60 min, B: 90 min, C: 120 min ; Reaction temperature, D: 20℃, E: 40℃, F: 60℃ ; Enzyme concentration, G: 0.05 mL/L, H: 0.15 mL/L, I: 0.25 mL/L)
표 Response optimization for mult-response surface of Novozym 33095 enzyme treatment from corn silk extracts
표 Central composite design for Pectinex Ultra SP-L enzyme treatment from corn silk extract and its dependent variables
표 Analysis of variance for the effects of three variables on total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Pectinex Ultra SP-L enzyme treatments from corn silk extracts
표 Regression coefficients of second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Pectinex Ultra SP-L enzyme treatments from corn silk extracts
표 Determination coefficients and probability second degree polymials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Pectinex Ultra SP-L enzyme treatments from corn silk extracts
표 Contour plot for total polyphenol contents (mg/100g) of Pectinex Ultra SP-L treatment from corn silk extracts (Reaction time, A: 2 hr, B: 5 hr, C: 8 hr ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.5 mL/100L, H: 1.0 mL/100L, I: 1.5 mL/100L)
표 Contour plot for total flavonoid contents (mg/100g) of Pectinex Ultra SP-L treatment from corn silk extracts (Reaction time, A: 2 hr, B: 5 hr, C: 8 hr ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.5 mL/100L, H: 1.0 mL/100L, I: 1.5 mL/100L)
표 Contour plot for maysin contents (mg/100g) of Pectinex Ultra SP-L treatment from corn silk extracts (Reaction time, A: 2 hr, B: 5 hr, C: 8 hr ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.5 mL/100L, H: 1.0 mL/100L, I: 1.5 mL/100L)
표 Contour plot for DPPH radical scavenging activity (%) of Pectinex Ultra SP-L treatment from corn silk extracts (Reaction time, A: 2 hr, B: 5 hr, C: 8 hr ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.5 mL/100L, H: 1.0 mL/100L, I: 1.5 mL/100L)
표 Contour plot for tyrosinase inhibition (%) of Pectinex Ultra SP-L treatment from corn silk extracts (Reaction time, A: 2 hr, B: 5 hr, C: 8 hr ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.5 mL/100L, H: 1.0 mL/100L, I: 1.5 mL/100L)
표 Response optimization for mult-response surface of Pectinex Ultra SP-L enzyme treatment from corn silk extracts
표 Central composite design for Celluclast 1.5L FG enzyme treatment from corn silk extract and its dependent variables
표 Analysis of variance for the effects of three variables on total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Celluclast 1.5L FG enzyme treatments from corn silk extracts
표 Regression coefficients of second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Celluclast 1.5L FG enzyme treatments from corn silk extracts
표 Determination coefficients and probability second degree polynomials for total polyphenol contents, total flavonoid contents, maysin contents, DPPH radical scavenging activity, tyrosinase inhibition of Celluclast 1.5L FG enzyme treatments from corn silk extracts
표 Contour plot for total polyphenol contents (mg/100g) of Celluclast 1.5L FG treatment from corn silk extracts (Reaction time, A: 40 min, B: 80 min, C: 120 min ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.25%, H: 0.50%, I: 0.75%)
표 Contour plot for total flavonoid contents (mg/100g) of Celluclast 1.5L FG treatment from corn silk extracts (Reaction time, A: 40 min, B: 80 min, C: 120 min ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.25%, H: 0.50%, I: 0.75%)
표 Contour plot for maysin contents (mg/100g) of Celluclast 1.5L FG treatment from corn silk extracts (Reaction time, A: 40 min, B: 80 min, C: 120 min ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.25%, H: 0.50%, I: 0.75%)
표 Contour plot for DPPH radical scavenging activity (%) of Celluclast 1.5L FG treatment from corn silk extracts (Reaction time, A: 40 min, B: 80 min, C: 120 min ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.25%, H: 0.50%, I: 0.75%)
표 Contour plot for tyrosinase inhibition (%) of Celluclast 1.5L FG treatment from corn silk extracts (Reaction time, A: 40 min, B: 80 min, C: 120 min ; Reaction temperature, D: 20℃, E:40℃, F: 60℃ ; Enzyme concentration, G: 0.25%, H: 0.50%, I: 0.75%)
표 Response optimization for mult-response surface of Celluclast 1.5L FG enzyme treatment from corn silk extracts
표 Instrument and analysis conditions for maysin
표 Scanning electron micrographs of the corn silk and micro-encapsulated corn silk extracts. A: control, B: Maltodextrin micro-encapsulated corn silk extracts, C: Cyclodextrin micro-encapsulated com silk extracts, D: Maltodextrin – Cyclodextrin micro-encapsulated com silk extracts
표 Mean particle size of corn silk and micro-encapsulated corn silk extracts
표 Water absorption index (WAI) and water solubility index (WSI) of micro-encapsulated corn silk extracts
표 Total polyphenol contents of corn silk and micro-encapsulated corn silk extracts
표 Total flavonoid contents of corn silk and micro-encapsulated corn silk extracts
표 Total maysin contents of corn silk and micro-encapsulated corn silk extracts
표 DPPH radical scavenging activity of corn silk and micro-encapsulated corn silk extracts
표 SOD-like activity of corn silk and micro-encapsulated corn silk extracts
표 Hydrogen peroxide scavenging activity of corn silk and micro-encapsulated corn silk extracts
표 Hydroxyl radical scavenging activity of corn silk and micro-encapsulated corn silk extracts
표 Elastase inhibition activity of corn silk and micro-encapsulated corn silk extracts
표 Tyrosinase inhibition activity of corn silk and micro-encapsulated corn silk extracts
표 Appearances of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃
표 Total polyphenol contents of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃. A: Control, B: Maltodextrin–Cyclodextrin micro-encapsulated com silk extracts
표 Total flavonoid contents of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃. A: Control, B: Maltodextrin – Cyclodextrin micro-encapsulated com silk extracts
표 Total maysin contents of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃. A: Control, B: Maltodextrin – Cyclodextrin micro-encapsulated com silk extracts
표 DPPH radical scarvenging of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃. A: Control, B: Maltodextrin – Cyclodextrin micro-encapsulated com silk extracts
표 SOD-like activity of corn silk and maltodextrin – cyclodextrin micro-encapsulated corn silk extracts during 5 weeks storage at 25℃. A: Control, B: Maltodextrin – Cyclodextrin micro-encapsulated com silk extracts
표 Formulas of yogurt dressing with corn silk concentrate
표 Formulas of muffin with corn silk concentrate
표 Procedures for preparation of muffins with corn silk concentrate
표 Formulas of Sikhe with corn silk concentrate
표 Procedures for preparation of Sikhe with corn silk concentrate
표 Instrument and analysis conditions for maysin
표 Viscosity, pH and sweetness of yogurt dressings with micro-encapsulated corn silk extracts
표 Yogurt dressings with micro-encapsulated corn silk. (control, CSC2, CSC4)
표 Color of yogurt dressings with micro-encapsulated corn silk extracts
표 Color of yogurt dressings with micro-encapsulated corn silk extracts. (A: control, B: CSC1, C: CSC2)
표 Total polyphenol contents of yogurt dressings with micro-encapsulated corn silk extracts
표 Total flavonoid contents of yogurt dressings with micro-encapsulated corn silk extracts
표 Maysin contents of yogurt dressings with micro-encapsulated corn silk extracts
표 DPPH radical scavenging activity of yogurt dressings with micro-encapsulated corn silk extracts
표 Hydroxyl radical scavenging activity of yogurt dressings with micro-encapsulated corn silk extracts
표 Hydrogen peroxide scavenging activity of yogurt dressings with micro-encapsulated corn silk extracts
표 Tyrosinase inhibition activity of yogurt dressings with micro-encapsulated corn silk extract
표 Sensory evaluation of yogurt dressings with micro-encapsulated corn silk extracts
표 pH and sweetness of muffin with micro-encapsulated corn silk extracts
표 Color of Muffin with micro-encapsulated corn silk extracts
표 Muffin dough with micro-encapsulated corn silk extracts. (control, CSC1, CSC2)
표 Total polyphenol contents of Muffin with micro-encapsulated corn silk extracts
표 Total flavonoid contents of Muffin with micro-encapsulated corn silk extracts
표 Maysin contents of Muffin with micro-encapsulated corn silk extracts
표 Texture profile analysis of Muffin with micro-encapsulated corn silk extracts
표 Muffin with micro-encapsulated corn silk. (control, CSC1, CSC2)
표 DPPH radical scavenging activity of muffin with micro-encapsulated corn silk extract
표 SOD-like activity of muffin with micro-encapsulated corn silk extracts
표 Hydroxyl radical scavenging activity of muffin with micro-encapsulated corn silk extracts
표 Hydrogen peroxide scavenging activity of muffin with micro-encapsulated corn silk extracts
표 Tyrosinase inhibition activity of Muffin with micro-encapsulated corn silk extract
표 Sensory evaluation of muffin with micro-encapsulated corn silk extracts
표 Viscosity and pH of Sikhe with micro-encapsulated corn silk extracts
표 Color of Sikhe with micro-encapsulated corn silk extracts
표 Sweet rice drink with micro-encapsulated corn silk. (control, CSC1, CSC2)
표 Total polyphenol contents of Sikhe with micro-encapsulated corn silk extracts
표 Total flavonoid contents of Sikhe with micro-encapsulated corn silk extracts
표 Maysin contents of Sikhe with micro-encapsulated corn silk extracts
표 DPPH radical scavenging activity of Sikhe with micro-encapsulated corn silk extracts
표 SOD-like activity of Sikhe with micro-encapsulated corn silk extracts
표 Hydroxyl radical scavenging activity of Sikhe with micro-encapsulated corn silk extracts
표 Hydrogen peroxide scavenging activity of Sikhe with micro-encapsulated corn silk extracts
표 Tyrosinase inhibition activity of Sikhe with micro-encapsulated corn silk extracts
표 Sensory evaluation of Sikhe with micro-encapsulated corn silk extracts

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안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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메이신 고함유 옥수수수염 추출물의 비임상효능 스크리닝 및 기전구명을 통한 기능성 제품실용화
Practical application of high concentration maysin corn silk extract through the investigation of biological efficacy and mechanism 원문보기

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메이신 고함유 옥수수수염 추출물의 비임상효능 스크리닝 및 기전구명을 통한 기능성 제품실용화 Practical application of high concentration maysin corn silk extract through the investigation of biological efficacy and mechanism 원문보기

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메이신 고함유 옥수수수염 추출물의 비임상효능 스크리닝 및 기전구명을 통한 기능성 제품실용화
Practical application of high concentration maysin corn silk extract through the investigation of biological efficacy and mechanism 원문보기

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