본 연구에서는 인체에 무해한 천연 항균 소재를 탐색하기 위한 목적으로 매실 농축액에 대하여 5종의 식중독균 (Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Sallmonela enteriditis, Bacillus cereus)과 2종의 무좀균 (...
본 연구에서는 인체에 무해한 천연 항균 소재를 탐색하기 위한 목적으로 매실 농축액에 대하여 5종의 식중독균 (Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Sallmonela enteriditis, Bacillus cereus)과 2종의 무좀균 (Trichophyton mentagrophytes, Microsporum audinii) 그리고 2종의 식물 병원균(Aspergillus flavus, Fusarium solani)에 대한 항균, 항진균 활성을 paper disc agar diffusion 법으로 측정하여 5가지 식중독균에 대한 항균효과를 확인하였고 항진균 효과는 없는 것으로 나타났다. 매실농축액의 최소저해농도는 농축원액대비 6.25%(w/v)의 농도에서 5가지 균주 모두 최초로 균의 증식이 저해되었다. 또한 매실농축액의 pH 안정성과 열안정성에 매우 우수한 것으로 나타났다. 매실농축액을 극성이 다른 4가지 용매(petroleum ether, chlorofrom, ethyl acetate, butanol)를 사용하여 분획한 결과 butanol fration이 가장 항균활성효과가 높은 것으로 나타났다. 항균활성효과가 가장 뛰어난 butanol 분획을 sepadex column chromatography와 silica gel column chromatography를 통해 분리하여 항균활성이 가장 큰 분획(Fr. 3-4)을 얻고 GC/MS로 분석한 결과 매실농축액의 butanol 분획물 중에 가장 큰 항균성을 나타내는 물질은 Trimethyl citrate 및 Hexanedioic acid일 가능성이 높은 것으로 확인되었다.Antimicrobial activity of Prunus mume extract was investigated as a natural antimicrobial source which is harmless to the human body. Prunus mume extract was evaluated for its antimicrobial properties against five species of bacteria (Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Salmonellaenteriditis, Bacillus cereus), The extract exerted a good antibacterialactivity against Vibrio parahaemolyticus와 Bacillus cereus by showing minimum inhibition concentration of 6.25%(v/v), which indicates that the activity of Prunus mume extract was equivalent as kanamycin (assume 16mg/ml as 100% concentration). However, antifungal activities are not exerted by the extract against two species of athlete's foot fungi (Trichophyton mentagrophytes and Microsporum audinii) and two plant pathogenic fungi (Aspergillus flavus, Fusarium solani). Slight decrease in antibacterial activity against Vibrio parahaemolyticus and Bacillus cereus was observed by changing the pH of the extract to pH 3, pH 7, and pH 11 causes. And the extract was stable at the heat treatment at 100℃ for 120min, and the that of 30min at 80℃~120℃. Prunus mume extract was sequentially partitioned into petroleum ether, chloroform, ethyl acetate, and butanol. Among four fractions, butanol fraction showed the highest antifungal activity against Vibrio parahaemolyticus and Bacillus cereus. Antibacterial compounds in butanol fraction were further purified by using Sephadex LH-20 column chromatography and silica gel open column chromatography. The highest antimicrobial activity against Vibrio parahaemolyticus and Bacillus cereus was acquired on the fraction 3-5. From the GC/MS analysis of the fraction 3-5, the materials showing higher antimicrobial activitiesin the butanol fraction were inferred trimethyl citrate and hexanedioic acid.
본 연구에서는 인체에 무해한 천연 항균 소재를 탐색하기 위한 목적으로 매실 농축액에 대하여 5종의 식중독균 (Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Sallmonela enteriditis, Bacillus cereus)과 2종의 무좀균 (Trichophyton mentagrophytes, Microsporum audinii) 그리고 2종의 식물 병원균(Aspergillus flavus, Fusarium solani)에 대한 항균, 항진균 활성을 paper disc agar diffusion 법으로 측정하여 5가지 식중독균에 대한 항균효과를 확인하였고 항진균 효과는 없는 것으로 나타났다. 매실농축액의 최소저해농도는 농축원액대비 6.25%(w/v)의 농도에서 5가지 균주 모두 최초로 균의 증식이 저해되었다. 또한 매실농축액의 pH 안정성과 열안정성에 매우 우수한 것으로 나타났다. 매실농축액을 극성이 다른 4가지 용매(petroleum ether, chlorofrom, ethyl acetate, butanol)를 사용하여 분획한 결과 butanol fration이 가장 항균활성효과가 높은 것으로 나타났다. 항균활성효과가 가장 뛰어난 butanol 분획을 sepadex column chromatography와 silica gel column chromatography를 통해 분리하여 항균활성이 가장 큰 분획(Fr. 3-4)을 얻고 GC/MS로 분석한 결과 매실농축액의 butanol 분획물 중에 가장 큰 항균성을 나타내는 물질은 Trimethyl citrate 및 Hexanedioic acid일 가능성이 높은 것으로 확인되었다.Antimicrobial activity of Prunus mume extract was investigated as a natural antimicrobial source which is harmless to the human body. Prunus mume extract was evaluated for its antimicrobial properties against five species of bacteria (Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Salmonellaenteriditis, Bacillus cereus), The extract exerted a good antibacterialactivity against Vibrio parahaemolyticus와 Bacillus cereus by showing minimum inhibition concentration of 6.25%(v/v), which indicates that the activity of Prunus mume extract was equivalent as kanamycin (assume 16mg/ml as 100% concentration). However, antifungal activities are not exerted by the extract against two species of athlete's foot fungi (Trichophyton mentagrophytes and Microsporum audinii) and two plant pathogenic fungi (Aspergillus flavus, Fusarium solani). Slight decrease in antibacterial activity against Vibrio parahaemolyticus and Bacillus cereus was observed by changing the pH of the extract to pH 3, pH 7, and pH 11 causes. And the extract was stable at the heat treatment at 100℃ for 120min, and the that of 30min at 80℃~120℃. Prunus mume extract was sequentially partitioned into petroleum ether, chloroform, ethyl acetate, and butanol. Among four fractions, butanol fraction showed the highest antifungal activity against Vibrio parahaemolyticus and Bacillus cereus. Antibacterial compounds in butanol fraction were further purified by using Sephadex LH-20 column chromatography and silica gel open column chromatography. The highest antimicrobial activity against Vibrio parahaemolyticus and Bacillus cereus was acquired on the fraction 3-5. From the GC/MS analysis of the fraction 3-5, the materials showing higher antimicrobial activitiesin the butanol fraction were inferred trimethyl citrate and hexanedioic acid.
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