This study was conducted to investigate feasibility of feedstuff for animal using food waste by fermentation mechanism of indigenous microorganism. To achieve this purpose, indigenous bacteria was isolated from manufactured feedstuff adding soils as an inoculant. Enzyme test was performed to verify ...
This study was conducted to investigate feasibility of feedstuff for animal using food waste by fermentation mechanism of indigenous microorganism. To achieve this purpose, indigenous bacteria was isolated from manufactured feedstuff adding soils as an inoculant. Enzyme test was performed to verify activity of amylase, protease, lipase and pectinase using isolated microorganisms. Microorganisms (4 species), which vigorously express the enzyme activity, was selected and used in the fermentation experiments of food waste. From the analysis of 16s rDNA sequencing, the microorganisms (4 species) identified as Bacillus subtilis (H1), Paenibacillus polymyxa (D1), Stenotrophomonas maltophilia (D4) and Burkholderia gladioli (D6). In the experiment, the main experimental material was food waste from restaurants, and rice bran, popped rice and dried food waste was added as a moisture controller. During fermentation 5 contents was measured by automatic system that is pH, DO, ORP, Salinity and Temperature. From the results of measured data, it can be verified that food waste adding the indigenous microorganisms was effectively fermented. From the nutritional analysis of manufactured feedstuff, it showed that the contents of crude protein, crude fat and crude fiber were enough to use as feedstuff for animal. In addition, harmful components such as Pb, Hg, Cd, aflatoxin and salmonella concentration were not exceeded permitted standards. Therefore, fermented food waste using indigenous microorganisms can be used as feedstuff.
This study was conducted to investigate feasibility of feedstuff for animal using food waste by fermentation mechanism of indigenous microorganism. To achieve this purpose, indigenous bacteria was isolated from manufactured feedstuff adding soils as an inoculant. Enzyme test was performed to verify activity of amylase, protease, lipase and pectinase using isolated microorganisms. Microorganisms (4 species), which vigorously express the enzyme activity, was selected and used in the fermentation experiments of food waste. From the analysis of 16s rDNA sequencing, the microorganisms (4 species) identified as Bacillus subtilis (H1), Paenibacillus polymyxa (D1), Stenotrophomonas maltophilia (D4) and Burkholderia gladioli (D6). In the experiment, the main experimental material was food waste from restaurants, and rice bran, popped rice and dried food waste was added as a moisture controller. During fermentation 5 contents was measured by automatic system that is pH, DO, ORP, Salinity and Temperature. From the results of measured data, it can be verified that food waste adding the indigenous microorganisms was effectively fermented. From the nutritional analysis of manufactured feedstuff, it showed that the contents of crude protein, crude fat and crude fiber were enough to use as feedstuff for animal. In addition, harmful components such as Pb, Hg, Cd, aflatoxin and salmonella concentration were not exceeded permitted standards. Therefore, fermented food waste using indigenous microorganisms can be used as feedstuff.
주제어
#Food waste fermentation Indigenous microorganisms Feedstuff
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