In this study antioxidant activity and antimicrobial activity of Psidium guajava leaf extracts using water, ethanol and methanol was compared and analyzed. Also investigated the influence of Guajava leaves extract for each ethanol concentration of 0, 30, 50, 70, 90% on the Staphylococcus aureus and ...
In this study antioxidant activity and antimicrobial activity of Psidium guajava leaf extracts using water, ethanol and methanol was compared and analyzed. Also investigated the influence of Guajava leaves extract for each ethanol concentration of 0, 30, 50, 70, 90% on the Staphylococcus aureus and in addition measured the anti-oxidative activity of each extract. In extract with water and ethanol at the concentration of 500 ppm or over it showed DPPH radical scavenging abilities as high as 90% or over and in reduction abilities and ABTs radical scavenging abilities it showed high activity in the order of water > ethanol > methanol extracts. As a result of measurement of nitric oxide (NO) scavenging abilities of Psidium guajava leaf extracts, activity of extract in ethanol appeared the highest, and in extract in water it showed about 50% activity at the concentration of 2,000 ppm, and in extract in methanol which showed activity of 30% or lower meaningful difference depending on concentration did not appear. Also in experiment of nitrite scavenging abilities in all experimental group, activity increased following the concentration but meaningful difference among experimental groups did not appear. As a result of study of antimicrobial activity of Psidium guajava leaf extracts, inhibition of growth of extract in water against atopy induction strain Staphylococcus aureus appeared very high, and in experiment on E. coli and dermatophyte Trichophyton rubrum and Trichophyton mentagrophyte it showed high antimicrobial activity in the order of water > ethanol > methanol extract. Extraction yield of each extract and content of index material polyphenol appeared highest in 50% ethanol extract as 16.78%, 187.19 mg/100 mL respectively and the content of flavonoid appeared highest in water extract. Anti-microorganism activity against Staphylococcus aureus of atopy inducing strain appeared in all experiment group as the size of clear zone is 20 mm or more. Expecially in concentration 0.1g/2ml of ethanol extract of 50% and 70% they were the highest as 24 mm or over and in the experiment of anti-microbial activity against E. coli they showed similar tendency. As a result of measuring DPPH and ABTs radical scavenging activity, there was tendency of scavenging activity increasing following the concentration of ethanol and in the concentration of higher than 250 μL/mL of concentration, high scavenging activity of more than 90% was shown. In addition, reducing power showed tendency of increasing depending on the concentration. Nitric oxide scavenging activity was showed the highest at the concentration of 100 μL/mL and scavenging activity of nitrite increased depending on the concentration of sample. In the comparative experiment following the concentration of ethanol on the whole the concentration of 50% ethanol showed the highest activity.
In this study antioxidant activity and antimicrobial activity of Psidium guajava leaf extracts using water, ethanol and methanol was compared and analyzed. Also investigated the influence of Guajava leaves extract for each ethanol concentration of 0, 30, 50, 70, 90% on the Staphylococcus aureus and in addition measured the anti-oxidative activity of each extract. In extract with water and ethanol at the concentration of 500 ppm or over it showed DPPH radical scavenging abilities as high as 90% or over and in reduction abilities and ABTs radical scavenging abilities it showed high activity in the order of water > ethanol > methanol extracts. As a result of measurement of nitric oxide (NO) scavenging abilities of Psidium guajava leaf extracts, activity of extract in ethanol appeared the highest, and in extract in water it showed about 50% activity at the concentration of 2,000 ppm, and in extract in methanol which showed activity of 30% or lower meaningful difference depending on concentration did not appear. Also in experiment of nitrite scavenging abilities in all experimental group, activity increased following the concentration but meaningful difference among experimental groups did not appear. As a result of study of antimicrobial activity of Psidium guajava leaf extracts, inhibition of growth of extract in water against atopy induction strain Staphylococcus aureus appeared very high, and in experiment on E. coli and dermatophyte Trichophyton rubrum and Trichophyton mentagrophyte it showed high antimicrobial activity in the order of water > ethanol > methanol extract. Extraction yield of each extract and content of index material polyphenol appeared highest in 50% ethanol extract as 16.78%, 187.19 mg/100 mL respectively and the content of flavonoid appeared highest in water extract. Anti-microorganism activity against Staphylococcus aureus of atopy inducing strain appeared in all experiment group as the size of clear zone is 20 mm or more. Expecially in concentration 0.1g/2ml of ethanol extract of 50% and 70% they were the highest as 24 mm or over and in the experiment of anti-microbial activity against E. coli they showed similar tendency. As a result of measuring DPPH and ABTs radical scavenging activity, there was tendency of scavenging activity increasing following the concentration of ethanol and in the concentration of higher than 250 μL/mL of concentration, high scavenging activity of more than 90% was shown. In addition, reducing power showed tendency of increasing depending on the concentration. Nitric oxide scavenging activity was showed the highest at the concentration of 100 μL/mL and scavenging activity of nitrite increased depending on the concentration of sample. In the comparative experiment following the concentration of ethanol on the whole the concentration of 50% ethanol showed the highest activity.
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