The enterocytes of the small intestine can only absorb monosaccharides such as glucose and fructose from our diet. The intestinal absorption of dietary carbohydrates such as maltose and sucrose is carried out by a group of α-glucosidases. Inhibition of these enzymes can significantly decrease the po...
The enterocytes of the small intestine can only absorb monosaccharides such as glucose and fructose from our diet. The intestinal absorption of dietary carbohydrates such as maltose and sucrose is carried out by a group of α-glucosidases. Inhibition of these enzymes can significantly decrease the postprandial increase of blood glucose level after a mixed carbohydrate diet. Therefore, the inhibitory activity of Zingiber mioga extracts against rat intestinal α-glucosidase (sucrase, maltase, glucoamylase) and porcine pancreatic α-amylase were studied in vitro and in vivo. Additional, oxygen radical absorbance capacity (ORAC) of Zingiber mioga extracts and what extend compounds isolated from Zingiber mioga could contribute to their antidiabetic activity are discussed. ZMJ had the highest α-glucosidase inhibitory activity (IC50, 6.99 mg/mL) followed by ZMW (IC50, 8.28 mg/mL), ZME (IC50, >10 mg/mL). ZMJ had also the highest sucrase inhibitory activity (IC50, >1.20 mg/mL) but ZME had the highest inhibitory activity on maltase, glucoamylase (IC50, 3.02 and 3.94 mg/mL) Followed by ZMW, ZMJ. Besides, ZMJ showed significant activation of α-amylase inhibitory activity (IC50, >10 mg/ml) and ZMJ has potent peroxyl radical scavenging linked antioxidant activity (2.73/TE 1 μM). The postprandial blood glucose lowering effect of Zingiber mioga extracts was compared to a known type 2 diabetes drug (Acarbose), a strong α-glucosidase inhibitor in the Sprague-Dawley (SD) rat model. In rats fed on sucrose, ZMJ significantly reduced the blood glucose increase after sucrose loading (ZMJ 0.1 g/kg 55.61 ± 13.24 (mg/dL)). Moreover, the six compounds purified from Zingiber mioga evaluated for α-glucosidase, sucrase inhibitory activity at a concentration of 1 mg/ml. Especially, 26A-ZM-3C exhibited strong inhibitory activities of 44.4%, 69.6% at the same concentration. Results suggest that, the α-glucosidase inhibitory activity and antioxidant activity in Zingiber mioga extract would be helpful to manage glucose uptake and the glucose-induced increased levels of mitochondrial reactive oxygen species (ROS) linked to hyperglycemia. This in vitro and in vivo study therefore could provide the biochemical rationale for the benefit of dietary supplement and the basis for further bioconversion studies.
The enterocytes of the small intestine can only absorb monosaccharides such as glucose and fructose from our diet. The intestinal absorption of dietary carbohydrates such as maltose and sucrose is carried out by a group of α-glucosidases. Inhibition of these enzymes can significantly decrease the postprandial increase of blood glucose level after a mixed carbohydrate diet. Therefore, the inhibitory activity of Zingiber mioga extracts against rat intestinal α-glucosidase (sucrase, maltase, glucoamylase) and porcine pancreatic α-amylase were studied in vitro and in vivo. Additional, oxygen radical absorbance capacity (ORAC) of Zingiber mioga extracts and what extend compounds isolated from Zingiber mioga could contribute to their antidiabetic activity are discussed. ZMJ had the highest α-glucosidase inhibitory activity (IC50, 6.99 mg/mL) followed by ZMW (IC50, 8.28 mg/mL), ZME (IC50, >10 mg/mL). ZMJ had also the highest sucrase inhibitory activity (IC50, >1.20 mg/mL) but ZME had the highest inhibitory activity on maltase, glucoamylase (IC50, 3.02 and 3.94 mg/mL) Followed by ZMW, ZMJ. Besides, ZMJ showed significant activation of α-amylase inhibitory activity (IC50, >10 mg/ml) and ZMJ has potent peroxyl radical scavenging linked antioxidant activity (2.73/TE 1 μM). The postprandial blood glucose lowering effect of Zingiber mioga extracts was compared to a known type 2 diabetes drug (Acarbose), a strong α-glucosidase inhibitor in the Sprague-Dawley (SD) rat model. In rats fed on sucrose, ZMJ significantly reduced the blood glucose increase after sucrose loading (ZMJ 0.1 g/kg 55.61 ± 13.24 (mg/dL)). Moreover, the six compounds purified from Zingiber mioga evaluated for α-glucosidase, sucrase inhibitory activity at a concentration of 1 mg/ml. Especially, 26A-ZM-3C exhibited strong inhibitory activities of 44.4%, 69.6% at the same concentration. Results suggest that, the α-glucosidase inhibitory activity and antioxidant activity in Zingiber mioga extract would be helpful to manage glucose uptake and the glucose-induced increased levels of mitochondrial reactive oxygen species (ROS) linked to hyperglycemia. This in vitro and in vivo study therefore could provide the biochemical rationale for the benefit of dietary supplement and the basis for further bioconversion studies.
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