맥문동 추출물이 여드름 유발균주에 미치는 영향 : Staphylococcus aureus와 Pseudomonas aeruginosa strain에 대한 영향 The effect of the Broadleaf Liriope extracts on acne-related bacteria : Strains : Staphylococcus aureus, Pseudomonas aeruginosa원문보기
본 연구에서는 여드름 유발 균주로 알려진 그램 양성균 Staphylococcus aureus KCTC 1927과, 그램 음성균 Pseudomonas aeruginosa KCTC 2004균주 두 종을 이용하여 맥문동 추출물이 여드름 균주에 미치는 영향에 대하여 측정하였다. 또한, 사람의 섬유아세포주인 HS-68 세포를 이용하여 사람 피부 세포에 대한 세포독성 효과를 측정 하였다. 맥문동 추출물을 얻기 위하여 깨끗이 세척한 맥문동 500 g을 ...
본 연구에서는 여드름 유발 균주로 알려진 그램 양성균 Staphylococcus aureus KCTC 1927과, 그램 음성균 Pseudomonas aeruginosa KCTC 2004균주 두 종을 이용하여 맥문동 추출물이 여드름 균주에 미치는 영향에 대하여 측정하였다. 또한, 사람의 섬유아세포주인 HS-68 세포를 이용하여 사람 피부 세포에 대한 세포독성 효과를 측정 하였다. 맥문동 추출물을 얻기 위하여 깨끗이 세척한 맥문동 500 g을 80 % 에탄올 1000 mL 에 넣고 100 ℃로 6시간 동안 교반 추출 하였다. 이러한 추출물 성분을 에탄올층과 물층, 유기층으로 분리하였다. 맥문동 추출물을 이용한 최소 저해 농도 측정법으로 측정하였을 때 Staphylococcus aureus 에서는 실험군으로 쓰인 DMSO와 비슷한 항균효과를 보였으며 그램 음성균 Pseudomonas aeruginosa 에서는 비교적 낮은 항균효과를 나타냈다. Disk paper method (Spread plate culture) 측정법으로 항균 활성을 측정했을 때, 그람 양성균인 Staphylococcus aureus 균주에서 에탄올층 4 mm, 유기층 2 mm, 물층 3 mm의 생육 저지환을 보이며 항균활성을 나타냈다. 그람 양성균인 Pseudomonas aeruginosa 균주에서는 에탄올층 6 mm, 유기층 5 mm, 물층 7 mm의 생육 저지환을 보이며 항균활성를 나타냈다. 맥문동 추출물이 항균활성을 보이는 농도에서 세포 독성이 있는지에 대한 실험을 하였다. HS-68 세포를 96 well plate에 4×10³ cells/ml 농도로 분주한 후 추출물을 처리하고 MTT 시약을 처리하여 세포 독성을 측정하였다. 항균활성을 보이는 농도에서는 세포독성이 없는 것으로 나타났다. 위의 결과로 볼 때 맥문동 추출물은 여드름균주의 성장을 억제하는 능력이 있는 것으로 나타났으며 피부세포에 대한 독성이 크지 않은 것으로 나타났다. 이에 맥문동은 화장품으로 사용가능한 안전한 항균제를 개발하는데 재료 물질이 될 수 있을 것으로 생각된다.
본 연구에서는 여드름 유발 균주로 알려진 그램 양성균 Staphylococcus aureus KCTC 1927과, 그램 음성균 Pseudomonas aeruginosa KCTC 2004균주 두 종을 이용하여 맥문동 추출물이 여드름 균주에 미치는 영향에 대하여 측정하였다. 또한, 사람의 섬유아세포주인 HS-68 세포를 이용하여 사람 피부 세포에 대한 세포독성 효과를 측정 하였다. 맥문동 추출물을 얻기 위하여 깨끗이 세척한 맥문동 500 g을 80 % 에탄올 1000 mL 에 넣고 100 ℃로 6시간 동안 교반 추출 하였다. 이러한 추출물 성분을 에탄올층과 물층, 유기층으로 분리하였다. 맥문동 추출물을 이용한 최소 저해 농도 측정법으로 측정하였을 때 Staphylococcus aureus 에서는 실험군으로 쓰인 DMSO와 비슷한 항균효과를 보였으며 그램 음성균 Pseudomonas aeruginosa 에서는 비교적 낮은 항균효과를 나타냈다. Disk paper method (Spread plate culture) 측정법으로 항균 활성을 측정했을 때, 그람 양성균인 Staphylococcus aureus 균주에서 에탄올층 4 mm, 유기층 2 mm, 물층 3 mm의 생육 저지환을 보이며 항균활성을 나타냈다. 그람 양성균인 Pseudomonas aeruginosa 균주에서는 에탄올층 6 mm, 유기층 5 mm, 물층 7 mm의 생육 저지환을 보이며 항균활성를 나타냈다. 맥문동 추출물이 항균활성을 보이는 농도에서 세포 독성이 있는지에 대한 실험을 하였다. HS-68 세포를 96 well plate에 4×10³ cells/ml 농도로 분주한 후 추출물을 처리하고 MTT 시약을 처리하여 세포 독성을 측정하였다. 항균활성을 보이는 농도에서는 세포독성이 없는 것으로 나타났다. 위의 결과로 볼 때 맥문동 추출물은 여드름균주의 성장을 억제하는 능력이 있는 것으로 나타났으며 피부세포에 대한 독성이 크지 않은 것으로 나타났다. 이에 맥문동은 화장품으로 사용가능한 안전한 항균제를 개발하는데 재료 물질이 될 수 있을 것으로 생각된다.
The purpose of this study was to measure the effects of extracts of liriope platyphylla herb on acne causing bacteria by using two kinds of bacteria such as gram positive Staphylococcus aureus KCTC 1927 and gram negative Pseudomonas aeruginosa KCTC 2004 which were known as acne causing bacteria. In ...
The purpose of this study was to measure the effects of extracts of liriope platyphylla herb on acne causing bacteria by using two kinds of bacteria such as gram positive Staphylococcus aureus KCTC 1927 and gram negative Pseudomonas aeruginosa KCTC 2004 which were known as acne causing bacteria. In addition, the cytotoxic effects on human skin cells were measured by using human fibroblast cell lines such as HS-68 cells. In order to obtain the extract of liriope platyphylla herb, after 500 g of liriope platyphylla herb was thoroughly washed, it was added into 1,000 mL of 80 % ethanol and extracted with stirring at 100 ℃ for 6 hours. These extracts were separated into ethanol phase, water phase and organic phase. When it was measured with minimal inhibitory concentration method by using the extracts of liriope platyphylla herb, the group with Staphylococcus aureus showed the anti-microbial effect which was similar to that of experimental group of DMSO. The group of Pseudomonas aeruginosa, gram negative bacterium showed a relatively low anti-microbial effect. When the anti-microbial activity was measured the disk paper method (spread plate culture), the group with Staphylococcus aureus, gram positive bacterium showed the growth inhibition zone of ethanol phase of 4 mm, organic phase of 2 mm and water phase of 3 mm and the anti-microbial activity. The group with Pseudomonas aeruginosa, gram negative bacterium showed the growth inhibition zone of ethanol phase of 6 mm, organic phase of 5 mm and water phase of 7 mm and the anti-microbial activity. The experiment was performed to examine whether the extracts of liriope platyphylla herb had cytotoxicity at the concentration showing the anti-microbial activity. After adding HS-68 cells into 96 well plate with concentration of 4×10³ cells/ml, they were treated with extracts. The cytotoxicity was measured by adding MTT reagent. It was found that there was no cytotoxicity at the concentration showing the anti-microbial activity. According to above results, it was found that the extracts of liriope platyphylla herb had the ability to inhibit the growth of acne bacteria and they did not have much toxicity to skin cells. Therefore, it is thought that the liriope platyphylla herb becomes the material to develop a safe anti-microbial agent being used as cosmetics.
The purpose of this study was to measure the effects of extracts of liriope platyphylla herb on acne causing bacteria by using two kinds of bacteria such as gram positive Staphylococcus aureus KCTC 1927 and gram negative Pseudomonas aeruginosa KCTC 2004 which were known as acne causing bacteria. In addition, the cytotoxic effects on human skin cells were measured by using human fibroblast cell lines such as HS-68 cells. In order to obtain the extract of liriope platyphylla herb, after 500 g of liriope platyphylla herb was thoroughly washed, it was added into 1,000 mL of 80 % ethanol and extracted with stirring at 100 ℃ for 6 hours. These extracts were separated into ethanol phase, water phase and organic phase. When it was measured with minimal inhibitory concentration method by using the extracts of liriope platyphylla herb, the group with Staphylococcus aureus showed the anti-microbial effect which was similar to that of experimental group of DMSO. The group of Pseudomonas aeruginosa, gram negative bacterium showed a relatively low anti-microbial effect. When the anti-microbial activity was measured the disk paper method (spread plate culture), the group with Staphylococcus aureus, gram positive bacterium showed the growth inhibition zone of ethanol phase of 4 mm, organic phase of 2 mm and water phase of 3 mm and the anti-microbial activity. The group with Pseudomonas aeruginosa, gram negative bacterium showed the growth inhibition zone of ethanol phase of 6 mm, organic phase of 5 mm and water phase of 7 mm and the anti-microbial activity. The experiment was performed to examine whether the extracts of liriope platyphylla herb had cytotoxicity at the concentration showing the anti-microbial activity. After adding HS-68 cells into 96 well plate with concentration of 4×10³ cells/ml, they were treated with extracts. The cytotoxicity was measured by adding MTT reagent. It was found that there was no cytotoxicity at the concentration showing the anti-microbial activity. According to above results, it was found that the extracts of liriope platyphylla herb had the ability to inhibit the growth of acne bacteria and they did not have much toxicity to skin cells. Therefore, it is thought that the liriope platyphylla herb becomes the material to develop a safe anti-microbial agent being used as cosmetics.
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