본 연구에서는 파프리카 수확 후 폐기되는 파프리카 잎의 70% 에탄올 추출물을 이용해 항산화, 항염증 및 항당뇨 효과를 검증해보고자 1,1-diphenyl -2-picrylhydrazyl (DPPH) 라디컬 소거활성, RAW 264.7 세포를 이용하여 ...
본 연구에서는 파프리카 수확 후 폐기되는 파프리카 잎의 70% 에탄올 추출물을 이용해 항산화, 항염증 및 항당뇨 효과를 검증해보고자 1,1-diphenyl -2-picrylhydrazyl (DPPH) 라디컬 소거활성, RAW 264.7 세포를 이용하여 lipopolysaccharide (LPS) 로 유도한 염증에 대해 염증성 cytokine과 염증성물질 변화를 조사하고, OGTT와 OSTT를 관찰하였다.
실험 결과 파프리카 잎 에탄올 추출물의 라디컬 소거능은 100~400 μg/mL의 농도에서 농도에 따라 증가하였고, 세포 독성은 100~400 μg/mL에서 세포독성은 나타나지 않았다. 또한 동일한 농도에서 LPS로 자극하여 nitric oxide (NO) 와 prostaglandin E2 (PGE2) 농도를 측정한 결과 농도 의존적으로 유의하게(p<0.05) 감소하였으며 Western blot 실험결과 inducible NO synthase (iNOS)와 cyclooxygenase-2 (COX-2)의 발현을 억제시켰고, tumor necrosis factor-α (TNF-α) 와 interleukin-6 (IL-6) 분비를 농도 의존적으로 감소하였으며 실시간중합효소반응 실험결과 TNF-α 와 IL-6 의 mRNA발현을 억제하였다. 당뇨 억제능을 확인하기위해 OGTT, OSTT 실험을 한 결과 파프리카 잎 추출물에서는 유의한 효과는 확인할 수 없었다.
이상의 결과 파프리카 잎 에탄올 추출물은 염증반응을 억제할 수 있는 천연물 소재로서 가치가 있다고 생각되어진다.
본 연구에서는 파프리카 수확 후 폐기되는 파프리카 잎의 70% 에탄올 추출물을 이용해 항산화, 항염증 및 항당뇨 효과를 검증해보고자 1,1-diphenyl -2-picrylhydrazyl (DPPH) 라디컬 소거활성, RAW 264.7 세포를 이용하여 lipopolysaccharide (LPS) 로 유도한 염증에 대해 염증성 cytokine과 염증성물질 변화를 조사하고, OGTT와 OSTT를 관찰하였다.
실험 결과 파프리카 잎 에탄올 추출물의 라디컬 소거능은 100~400 μg/mL의 농도에서 농도에 따라 증가하였고, 세포 독성은 100~400 μg/mL에서 세포독성은 나타나지 않았다. 또한 동일한 농도에서 LPS로 자극하여 nitric oxide (NO) 와 prostaglandin E2 (PGE2) 농도를 측정한 결과 농도 의존적으로 유의하게(p<0.05) 감소하였으며 Western blot 실험결과 inducible NO synthase (iNOS)와 cyclooxygenase-2 (COX-2)의 발현을 억제시켰고, tumor necrosis factor-α (TNF-α) 와 interleukin-6 (IL-6) 분비를 농도 의존적으로 감소하였으며 실시간중합효소반응 실험결과 TNF-α 와 IL-6 의 mRNA발현을 억제하였다. 당뇨 억제능을 확인하기위해 OGTT, OSTT 실험을 한 결과 파프리카 잎 추출물에서는 유의한 효과는 확인할 수 없었다.
이상의 결과 파프리카 잎 에탄올 추출물은 염증반응을 억제할 수 있는 천연물 소재로서 가치가 있다고 생각되어진다.
Capsicum annuum L. (Solanaceae), a species of the plant genus Capsicum, is a perennial herbaceous plants and features characteristically less pungency with a sweet taste, often used for salad and meat spice. It contains good levels of vitamin A, B1 and C.
The aim of the present study is to evaluat...
Capsicum annuum L. (Solanaceae), a species of the plant genus Capsicum, is a perennial herbaceous plants and features characteristically less pungency with a sweet taste, often used for salad and meat spice. It contains good levels of vitamin A, B1 and C.
The aim of the present study is to evaluate anti-oxidant, anti-inflammatory and antidiabetic effects of the 70%-ethanol extract from leaves of C. annuum (CALE). Anti-oxidant effect of CALE was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. For evaluation of anti-inflammatory effect of CALE, RAW264.7 macrophages were stimulated with the endotoxin lipopolysaccharide (LPS) in the absence or presence of CALE, and the expression of the proteins of inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNAs of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the production of NO, prostaglandin E2 (PGE2), TNF-α and IL-6. In C57BL/6 mice, oral glucose tolerance test (OGTT) and oral sucrose tolerance test (OSTT) were carried out to evaluate the antidiabetic effect of CALE. The concentration for the 50% of DPPH radical scavenging activity of CALE was found to be 400 μg/mL, as opposed to that of ascorbic acid (50% at 55.89 μg/mL), which is a well known anti-oxidant. In LPS- stimulated RAW264.7 macrophages, CALE (100~400 μg/mL) reduced NO and PGE2 production by suppressing iNOS and COX-2 expression and also decreased TNF-α and IL-6 secretion by inhibiting TNF-α and IL-6 mRNAs. In mice, CALE did not inhibit the increase in blood glucose level after either sucrose or glucose loading, while metformin, an OGTT control drug, and acarbose, an OSTT control drug, significantly inhibited the increase in glucose level. The results observed in this study suggest that CALE has anti-oxidant and anti-inflammatory effects, but no antidiabetic effect.
Capsicum annuum L. (Solanaceae), a species of the plant genus Capsicum, is a perennial herbaceous plants and features characteristically less pungency with a sweet taste, often used for salad and meat spice. It contains good levels of vitamin A, B1 and C.
The aim of the present study is to evaluate anti-oxidant, anti-inflammatory and antidiabetic effects of the 70%-ethanol extract from leaves of C. annuum (CALE). Anti-oxidant effect of CALE was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. For evaluation of anti-inflammatory effect of CALE, RAW264.7 macrophages were stimulated with the endotoxin lipopolysaccharide (LPS) in the absence or presence of CALE, and the expression of the proteins of inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNAs of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the production of NO, prostaglandin E2 (PGE2), TNF-α and IL-6. In C57BL/6 mice, oral glucose tolerance test (OGTT) and oral sucrose tolerance test (OSTT) were carried out to evaluate the antidiabetic effect of CALE. The concentration for the 50% of DPPH radical scavenging activity of CALE was found to be 400 μg/mL, as opposed to that of ascorbic acid (50% at 55.89 μg/mL), which is a well known anti-oxidant. In LPS- stimulated RAW264.7 macrophages, CALE (100~400 μg/mL) reduced NO and PGE2 production by suppressing iNOS and COX-2 expression and also decreased TNF-α and IL-6 secretion by inhibiting TNF-α and IL-6 mRNAs. In mice, CALE did not inhibit the increase in blood glucose level after either sucrose or glucose loading, while metformin, an OGTT control drug, and acarbose, an OSTT control drug, significantly inhibited the increase in glucose level. The results observed in this study suggest that CALE has anti-oxidant and anti-inflammatory effects, but no antidiabetic effect.
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