송이균의 환경조건에 따른 생장특성을 구명하고자 송이균을 고체매질을 이용하여 배양하였다. 고체매질에서의 송이균의 생장량은 에르고스테롤 함량을 분석하여 비교하였다. 송이균의 배양에 적합한 고체매질은 펄라이트와 마사토로 나타났으며 곡물 영양분으로는 보리가 우수하였다. 적정화된 고체배지에서 송이균의 생장은 송이균의 접종 후 2주부터 급격히 생장하였으며 8주 경에 최고의 생장량을 나타냈다. 송이균 배양에 적합한 수분함량은 30~70%였으며 부엽토 첨가(10%)가 생장에 더욱 양호하였다. 이러한 결과는 송이산지에서 토양유기물이 적절히 있고 토양수분이 높을 때에 송이균의 생장이 왕성할 것으로 추정할 수 있다. 고체배지에서 송이균사체를 접종 후 2주부터 급격히 생장하였으며 8주 경에 최고의 생장량을 나타냈다. 이러한 고체매질은 앞으로 송이 균사체의 생리연구에 이용될 수 있을 것이다.
송이균의 환경조건에 따른 생장특성을 구명하고자 송이균을 고체매질을 이용하여 배양하였다. 고체매질에서의 송이균의 생장량은 에르고스테롤 함량을 분석하여 비교하였다. 송이균의 배양에 적합한 고체매질은 펄라이트와 마사토로 나타났으며 곡물 영양분으로는 보리가 우수하였다. 적정화된 고체배지에서 송이균의 생장은 송이균의 접종 후 2주부터 급격히 생장하였으며 8주 경에 최고의 생장량을 나타냈다. 송이균 배양에 적합한 수분함량은 30~70%였으며 부엽토 첨가(10%)가 생장에 더욱 양호하였다. 이러한 결과는 송이산지에서 토양유기물이 적절히 있고 토양수분이 높을 때에 송이균의 생장이 왕성할 것으로 추정할 수 있다. 고체배지에서 송이균사체를 접종 후 2주부터 급격히 생장하였으며 8주 경에 최고의 생장량을 나타냈다. 이러한 고체매질은 앞으로 송이 균사체의 생리연구에 이용될 수 있을 것이다.
This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as t...
This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as the growth index of the mycelia. Among the various solid matrixes, the granitic soil, perlite and mixture of the two supported the growth most. Barely flour appeared to be very effective on the stimulating of the mycelial growth when added to the solid matrix. An mixture of the matrix contained an even (1:1:1:1, v/v/v/v) mixture of granitic soil, perlite, vermiculate and pine sawdust. T. matsutake started growth 2 weeks after inoculation and reached stationary growth phase after 8th weeks in the solid matrix mixture. The mycelial density in the solid matrix was 7 times higher than that in fairy-ring soil. In addition, 30~70% water content and 10% humus soil in the solid matrix also supported good growth suggesting that T. matsutake needs humus soil for a nutrient sources. The solid matrix developed in the present study could be used to study physiological characteristics of T. matsutake as well.
This study was conducted to develop optimal solid culture medium for Tricholoma matsutake. As the solid matrix, granitic soil, perlite, vermiculate, pine sawdust and peat moss were compared regarding their effected on mycelial growth. Ergosterol content which is a fungal wall component was used as the growth index of the mycelia. Among the various solid matrixes, the granitic soil, perlite and mixture of the two supported the growth most. Barely flour appeared to be very effective on the stimulating of the mycelial growth when added to the solid matrix. An mixture of the matrix contained an even (1:1:1:1, v/v/v/v) mixture of granitic soil, perlite, vermiculate and pine sawdust. T. matsutake started growth 2 weeks after inoculation and reached stationary growth phase after 8th weeks in the solid matrix mixture. The mycelial density in the solid matrix was 7 times higher than that in fairy-ring soil. In addition, 30~70% water content and 10% humus soil in the solid matrix also supported good growth suggesting that T. matsutake needs humus soil for a nutrient sources. The solid matrix developed in the present study could be used to study physiological characteristics of T. matsutake as well.
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