오동나무잎, 열매, 내수피, 외수피 그리고 목질부를 채취하여 건조시킨 후 분말로 제조하여 각 시료 약 5.0 kg을 아세톤-물(7:3. v/v) 혼합용액으로 추출하고 유기용매를 제거한 후 헥산, 메틸렌클로라이드, 에틸아세테이트 및 물 등 네 개의 분획으로 분리하였다. 오동나무 각 부위별 추출물 수율은 내수피(11.35%)가 가장 높았고 잎(7.87%), 열매(6.94%), 외수피(4.24%)의 순 이었으며 목질부가 4.10%로 가장 낮게 나타났다. 오동나무 각 부위별 조추출물, 고형분과 분획물을 가지고 DPPH radical 소거법을 이용하여 기초적인 항산화 실험을 실시하였다. 기준물질로는 천연 항산화제인 ${\alpha}$-tocopherol과 합성항산화제인 BHT를 사용하여 항산화효능을 비교하였다. 그 중에서는 모든 EtOAc용성 분획이 기준물질 보다 더 우수한 항산화 활성을 나타내었다.
오동나무잎, 열매, 내수피, 외수피 그리고 목질부를 채취하여 건조시킨 후 분말로 제조하여 각 시료 약 5.0 kg을 아세톤-물(7:3. v/v) 혼합용액으로 추출하고 유기용매를 제거한 후 헥산, 메틸렌클로라이드, 에틸아세테이트 및 물 등 네 개의 분획으로 분리하였다. 오동나무 각 부위별 추출물 수율은 내수피(11.35%)가 가장 높았고 잎(7.87%), 열매(6.94%), 외수피(4.24%)의 순 이었으며 목질부가 4.10%로 가장 낮게 나타났다. 오동나무 각 부위별 조추출물, 고형분과 분획물을 가지고 DPPH radical 소거법을 이용하여 기초적인 항산화 실험을 실시하였다. 기준물질로는 천연 항산화제인 ${\alpha}$-tocopherol과 합성항산화제인 BHT를 사용하여 항산화효능을 비교하였다. 그 중에서는 모든 EtOAc용성 분획이 기준물질 보다 더 우수한 항산화 활성을 나타내었다.
Approximately each 5 kg dried Paulownia coreana leaves, seeds, outer barks, inner barks and wood were ground, extracted with acetone-$H_2O$ (7:3. v/v). concentrated under reduced pressure and successively fractionated using n-hexane. $CH_2Cl_2$, EtOAc and $H_2O$ on a...
Approximately each 5 kg dried Paulownia coreana leaves, seeds, outer barks, inner barks and wood were ground, extracted with acetone-$H_2O$ (7:3. v/v). concentrated under reduced pressure and successively fractionated using n-hexane. $CH_2Cl_2$, EtOAc and $H_2O$ on a separation funnel. Each fraction was then freeze dried and weighed. The extraction yield of each sample was investigated and the yield of inner barks was the highest, while the wood was the lowest. Antioxidative activities of crude extracts, precipitates and partitioned fractions were evaluated by DPPH radical scavenging method. The results indicated that all EtOAc soluble fractions, including some of $H_2O$ soluble fractions, showed significantly high antioxidative potential compared with ${\alpha}$-tocopherol and BHT used as controls.
Approximately each 5 kg dried Paulownia coreana leaves, seeds, outer barks, inner barks and wood were ground, extracted with acetone-$H_2O$ (7:3. v/v). concentrated under reduced pressure and successively fractionated using n-hexane. $CH_2Cl_2$, EtOAc and $H_2O$ on a separation funnel. Each fraction was then freeze dried and weighed. The extraction yield of each sample was investigated and the yield of inner barks was the highest, while the wood was the lowest. Antioxidative activities of crude extracts, precipitates and partitioned fractions were evaluated by DPPH radical scavenging method. The results indicated that all EtOAc soluble fractions, including some of $H_2O$ soluble fractions, showed significantly high antioxidative potential compared with ${\alpha}$-tocopherol and BHT used as controls.
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대상 데이터
The leaves, seeds, inner barks, outer barks and wood of P. coreana (10 years old, DBH, 35 cm) were collected from the experimental forest of Kangwon National University in September and October of 2002. A voucher specimen of each sample was deposited in the Department of Wood Science and Engineering, College of Forest Science, Kangwon National University.
coreana (10 years old, DBH, 35 cm) were collected from the experimental forest of Kangwon National University in September and October of 2002. A voucher specimen of each sample was deposited in the Department of Wood Science and Engineering, College of Forest Science, Kangwon National University. Sample of each part was air-dried at room temperature for three weeks and then ground to fine powder by Wiley mill to be extracted.
성능/효과
Results indicated that the antioxidative potentials of the crude extracts of leaves (121 μg), outer barks (33 μg), inner barks (32 μg), seeds (31 μg) and wood (27 μg) were more effective. Results also indicated that the EtOAc soluble fractions of all samples and the H2O soluble fractions of seeds (25 μg), wood (21 μg) and inner barks (17 μg) exhibited significantly high activities, while the rest fractions, crude extracts and precipitates showed weak or none activities compared with BHT (30 μg) and α-tocopherol (26 μg). Therefore, most of the EtOAc soluble fractions of P.
values) on the crude extracts, precipitates and solvent partitioned fractions of leaves, seeds, outer barks, inner barks and wood were shown in Table 2. Results indicated that the antioxidative potentials of the crude extracts of leaves (121 μg), outer barks (33 μg), inner barks (32 μg), seeds (31 μg) and wood (27 μg) were more effective. Results also indicated that the EtOAc soluble fractions of all samples and the H2O soluble fractions of seeds (25 μg), wood (21 μg) and inner barks (17 μg) exhibited significantly high activities, while the rest fractions, crude extracts and precipitates showed weak or none activities compared with BHT (30 μg) and α-tocopherol (26 μg).
The extraction yield of each sample was investigated and the yield of the inner bark was the highest (11.35%), while the wood was the lowest (4.10%). The DPPH radical scavenging effect of the samples indicated that all the EtOAc soluble fractions, including some of the H2O soluble fractions, showed significantly high antioxidative potential compared with BHT and α-tocopherol as controls.
10%). The DPPH radical scavenging effect of the samples indicated that all the EtOAc soluble fractions, including some of the H2O soluble fractions, showed significantly high antioxidative potential compared with BHT and α-tocopherol as controls. This fact suggested that the extracts of P.
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