[논문]국산 녹차의 채취시기별 활성물질 및 항산화능 변화

강성구; 손미예

국산 녹차의 채취시기별 활성물질 및 항산화능 변화
Changes of Bioactive Compounds and Antioxidant Activities in Korean Green Tea (Camellia sinensis) with Different Harvesting Periods 원문보기

한국식품저장유통학회지 = Korean journal of food preservation, v.14 no.6, 2007년, pp.709 - 715

초록
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국산 녹차의 채취시기별 주요 화학성분과 항산화 특성을 평가하기 위하여 폴리페놀, 플라보노이드, 데아닌, 유리아미노산 함량과 항산화 및 라디칼 소거능에 대하여 조사하였다. 총 폴리페놀 함량은 중작 37.16 mg/g로 가장 높았으며, 다음으로 세작 24.65 mg/g, 대작 23.28 mg/g, 우전 19.55 mg/g 순이었으며, 총 플라보노이드는 대작이 350.83 mg%으로 중작 270.36 mg%, 세작 256.88 mg%, 우전 220.81 mg%에 비하여 비교적 높았다. Theanine과 총 유리아미노산은 우전에서 각각 14.11 mg/g, 23.62 mg/g으로 가장 높았으며, 이후에 나오는 녹차는 그 함량이 점진적으로 감소되었다. 또한 수확시기에 따른 항산화능을 linoleic acid peroxidation, DPPH 및 ABTS 자유라디칼 소거능으로 조사한 결과, 녹차 중 중작이나 대작에서는 높았지만, 우전과 세작은 항산화 활성과 자유라디칼 소거능이 거의 없었고, 그 환원력은 농도 의존적으로 증가하였다. 이상의 결과로부터 중작과 대작의 항산화능과 자유라디칼 소거능은 총 폴리페놀과 총 플라보노이드 함량에 관련되는 것으로 판단된다.

Abstract ▼ AI-Helper

Korean green tea has been claimed to have health-promoting effects, which may be related to the antioxidant activity in vitro. Korean green teas (Woojeon, WJ ; Sejak, SJ ; Jungjak, JJ ; Daejak, DJ) were subjected to different harvested times and yet little research has examined their bioactive compounds. To assess the effect of this different harvested times on nutritional and health-related properties such as Korean green tea polyphenols, flavonoids, theanine and free amino acids, antioxidant activities and radical scavenging activities were determined. Total polyphenols in JJ (37.16 mg/g) was higher than in other products (WJ, 19.55 ; SJ 24.65 ; DJ, 23.28 mg/g). Contents of flavonol and flavone glycosides were the highest at DJ (350.83 mg%) as compared to WJ (220.81), SJ (256.88) and JJ (270.36 mg%). Contents of theanine and total free amino acids were the highest at WJ (14.11, 23.62 mg/g, respectively), but decreased thereafter. Antioxidant activities were higher in JJ and DJ, using the linoleic acid peroxidation, DPPH and ABTS free radical-scavenging activities. However, WJ and SJ had less active antioxidant activity and free radical-scavenging activity. Reducing powers were increased depend on the concentration of extracts. Antioxidant activity and free radical-scavenging activity of JJ and DJ seemed to relate with total polyphenol and flavonoid contents.

주제어

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문제 정의

The objective of this study was to investigate the changes and antioxidant activities of polyphenols and flavonoids during different harvesting periods for the purposes of maximizing the nutraceutical benefits of green tea consumption.

대상 데이터

(—)-Gallocatechin (GC), (-)-epigallocatechin (EGC), (-)-gallocatechin gallate (GCG) and (—)-epigallocatechin gallate (EGCG), quercetin, kaempherol, luteolin, apigenine, theanine, linoleic acid, potassium phosphate, potassium ferricyanide, ferric chloride and l-ascorbic acid, 2, 2-diphenyl -picrylhydrazyl (DPPH), 2, 2-azinobis (3-ethylbenzothiazoline- 6-sulfonate) (ABTS) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All other solvents / chemicals used were of analytical grade and purchased from Sigma- Aldrich Co.

데이터처리

The results were expressed as means±SD using Microsoft Excel software. Statistical comparisons were made by ANOVA procedure followed by a Duncan’s multiple range tests, p<0.05 were considered significantly different.
Results are means±SD of three measurements. Values with different superscripts within the all column and row are significantly different at p<0.05 by Duncan's multiple range test.

이론/모형

3. Antioxidant activities of green tea in the linoleic acid emulsion system using the thiocyanate method.
1. Free radical scavenging activities of green tea as determined by the DPPH method.
The inhibition of linoleic acid peroxidation was measured by the method of Mitsuda et al. (20). Linoleic acid emulsion was prepared by adding equal amount of linoleic acid and tween 20, followed by serially dilution to a final concentration of 0.
The reducing power of extracts were determined by the method of Yen and Duh (21). Different concentrations of extracts were mixed with 2.
The scavenging activity of methanol extracts on DPPH radicals was measured according to the method of Yoshida et al. (18). Ethanolic solutions of DPPH (10-4M) and methanol extracts solutions were mixed so that the final mass ratios were extracts : DPPH.

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