[논문]New Finding and Optimal Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178

Lee, Geon-Ho; Bae, Jae-Han; Suh, Min-Jung; Kim, In-Hwan; Hou, Ching T.; Kim, Hak-Ryul

[국내논문] New Finding and Optimal Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178 원문보기

Journal of microbiology and biotechnology, v.17 no.6, 2007년, pp.1054 - 1057

Lee, Geon-Ho (Department of Animal Science and Biotechnology, Kyungpook National University) , Bae, Jae-Han (Department of Animal Science and Biotechnology, Kyungpook National University) , Suh, Min-Jung (Department of Animal Science and Biotechnology, Kyungpook National University) , Kim, In-Hwan (Department of Food and Nutrition, College of Health Sciences, Korea University) , Hou, Ching T. (Microbial Genomic and Bioprocessing Research Unit, National Center for Agricultural Utilization Research) , Kim, Hak-Ryul (Department of Animal Science and Biotechnology, Kyungpook National University)

Abstract ▼ AI-Helper

Lipases are industrially useful versatile enzymes that catalyze numerous different reactions including hydrolysis of triglycerides, transesterification, and chiral synthesis of esters under natural conditions. Although lipases from various sources have been widely used in industrial applications, such as in food, chemical, pharmaceutical, and detergent industries, there are still substantial current interests in developing new microbial lipases, specifically those functioning in abnormal conditions. We screened 17 lipase-producing yeast strains, which were prescreened for substrate specificity of lipase from more than 500 yeast strains from the Agricultural Research Service Culture Collection (Peoria, IL, U.S.A.), and selected Yarrowia lipolytica NRRL Y-2178 as a best lipase producer. This report presents new finding and optimal production of a novel extracellular alkaline lipase from Y. lipolytica NRRL Y-2178. Optimal culture conditions for lipase production by Y. lipolytica NRRL Y-2178 were 72 h incubation time, $27.5^{\circ}C$, pH 9.0. Glycerol and glucose were efficiently used as the most efficient carbon sources, and a combination of yeast extract and peptone was a good nitrogen source for lipase production by Y. lipolytica NRRL Y-2178. These results suggested that Y. lipolytica NRRL Y-2178 shows good industrial potential as a new alkaline lipase producer.

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가설 설정

lipolytica NRRL Y-2178. L:pase production and cell growth with other organic and inorganic nitrogen sources were very low.

제안 방법

In this study, we expanded our research more specifically with respect to high production and novel properties of lipase from Yarrowia lipolytica NRRL Y-2178, which was selected as a best lipase producer among yeast strains tested.

이론/모형

Dry cell weight (DCW) was calculated from the absorbance value using a standard curve. Lipase activity was estimated by a spectrophotometric method [20] with p-nitrophenylbutyrate (p-NPB) as the substrate. The assay mixture was composed of 0.

성능/효과

0 [1]. All these results were quite diflferent from our results, confirming that lipase from Y. lipolytica NRRL Y-2178 was a novel alkaline lipase.
Interestingly, when peptone and yeast extract were combined ina 1:1 ratio, lipase production dramatically increased, reaching up to the control. These results suggested that yeast extract was important for cell growth, whereas peptone played a certain important role in enhancing lipase production, and that there could be a possible synergistic relationship involved between the two nitrogen sources in lipase production by Y. lipolytica NRRL Y-2178. L:pase production and cell growth with other organic and inorganic nitrogen sources were very low.
From this study, we confirmed that extracellular lipase from Y. lipolytica NRRL Y-2178 was a novel alkaline lipase and that sorae of the optimal culture conditions for lipase production by Y. lipolytica NRRL Y-2178 were quite different from other Yarrowia species. Glycerol and glucose were efficiently used as the most efficient carbon sources, and a combination of yeast extract and peptone was a good nitrogen source for lipase production by Y.

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[국내논문] New Finding and Optimal Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178 원문보기

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[국내논문] New Finding and Optimal Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178 원문보기

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