[논문]흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교

이현화; 이숙영

흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교
Cytotoxic and Antioxidant Effects of Taraxacum coreanum Nakai. and T. officinale WEB. Extracts 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.16 no.2, 2008년, pp.79 - 85

이현화 (조선대학교 자연과학대학 생물학과) , 이숙영 (조선대학교 단백질소재연구센터)

Abstract ▼ AI-Helper

Some studies of dandelion have been carried out on bioactivities, however, no comparative analysis on antioxidant and cytotoxic activities in the Korean dandelion (T. coreanum NAKAI.; KD) and dandelion (T. officinale WEB.) have been reported. In this study, the extracts of KD and dandelion analyzed relative phenolic contents and free radical scavenging, antioxidant enzyme and cytotoxic activities. The extract of Korean dandelion leaves (KDL) exhibited a higher phenolics content ($368{\pm}11.5\;mg/l00\;g$) and a strong free radical scavenging activity ($RC_{50}$value;87.89 ug/ml) than other parts and BHT (120.12 ug/ml), synthetic antioxidant. At ascorbate peroxidase (APX) activity, the dandelion root (DR) had a greater (38.8 U/mg protein) antioxidant enzyme compare to the dandelion leaves (12.2 U/mg protein). The catalase (CAT) and superoxide dismutase (SOD) followed higher enzyme activity in Korean dandelion root (KDR) than other parts. In a cytotoxic activity against human cancer cell, the extracts of KDR was found to be active against Calu-6, HCT-116, and SNU-601 cell lines, with $IC_{50}$ values of 522.34, 532.74 and 614.85 ug/ml, respectively. These results suggest that KD and dandelion would be an alternative antioxidant source, based on natural plant resources.

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제안 방법

In this study we evaluated the total phenolic content of ethanolic extract of KDL, KDR, dandelion leaves (DL) and DR (Table 1). All the extracts contained relatively high total phenolics (130-368 mg/100 g).

데이터처리

experiments. Statistical differences were tested for significance using the student t-test (SPSS for Windows 12.0 package, SPSS Inc.).The values of p lower than 0.

이론/모형

Total phenolic content of extracts was determined according to the Folin-Ciocalteu method (Kahkonen et al., 1999). 700 ul of Folin-Ciocalteu reagent was added to 50 ul of extract and stirred vigorously by vortex and left to stand for 3 min.

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흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교
Cytotoxic and Antioxidant Effects of Taraxacum coreanum Nakai. and T. officinale WEB. Extracts 원문보기

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흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교 Cytotoxic and Antioxidant Effects of Taraxacum coreanum Nakai. and T. officinale WEB. Extracts 원문보기

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흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교
Cytotoxic and Antioxidant Effects of Taraxacum coreanum Nakai. and T. officinale WEB. Extracts 원문보기

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