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제안 방법
- It has been very meticulously demonstrated earlier that in the case of pelleted MBGs at higher enzyme loading, because of the diffiisional limitation of substrates/ products, the surface catalysis wo니d predominate resulting in inefficient use of the enzyme [16]. In order to study the same phenomenon, we compared the esterification efficiency and reusability of untreated pelleted and granulated MBGs at equal enzyme loading, where MBGs were intermittently treated with dry reverse micellar solution of 0.2 M AOT in isooctane after every 3 cycles. The total surface area of pelleted and granulated MBGs was found to be 2.
- However, in contrast to untreated MBGs, the esterification eflficiency of pretreated MBGs declined or decreased slowly after 5 cycles, with about 50% reduction in esterification activity at the end of the 12th cycle. The anunmt of water extracted during pretreatment of MBGs with dry reverse micellar solution was monitored by Karl-Fischer titration and the R values of MBGs were calculated. The R value of freshly prepared MBGs was reduced from ca.
- The authors are also gratefill to the Sophisticated Instrumentation Centre for Applied Research and Testing (SICART), Vallabh Vidyanagar, India, for providing the instrumentation facility.
대상 데이터
- (Steinheim, Germany). Cetyl trimethyl ammonium bromide (CTAB) was obtained from Sisco Chem Industries (India). Ethyl isovalerate was purchased from Aldrich.
- Cetyl trimethyl ammonium bromide (CTAB) was obtained from Sisco Chem Industries (India). Ethyl isovalerate was purchased from Aldrich. Gelatin from porcine skin (Bloom 300) and Candida rugosa lipase (triacylglycerol acyl hydrolase, E.
이론/모형
- 39 after treatment of the gel with 1 M AOT/isooctane reverse micellar solution, which indicates removal of free water from the gel. The amount of water extracted from the gel in dry reverse micellar solution was monitored using Karl-Fischer titration and used to calculate R values of MBGs. The R value of MBGs increased from 60 (initial) to 82.
- The water extracted from the MBGs in AOT/isooctane solution was determined by the Karl-Fischer method using Hydranal-E reagent by volumetric titration.
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