옻(Rhus verniciflua Stokes)으로 부터 분리한 알레르기 유발물질의 BALB/c mice에 대한 특성 Characteristics of Allergy Inducing Materials Isolated from Rhus verniciflua Stokes on BALB/c Mice원문보기
옻 allergy 유발물질을 분리하고 그 특성을 연구하기 위하여 국내산, 중국산 및 일본산 생 옻을 사용하였다. 옻의 crude extracts로부터 crude urushiol을 정제하고, 다시 allergy 유발물질로 알려져 있는 3종의 urushiol congeners를 분리하였다. 분리한 urushiol congeners는 RV-1 였으며, 전체의 80%에 해당하였다. RV-1의 함량은 중국산에서 70.07%로 가장 많았으며, 일본산에서 62.38%로 가장 적었다. 반면에 RV-2는 일본산은 9.25%로서 국내산과 중국산의 4.28 및 3.09%에 비하여 약 2$\sim$3 배정도 많았다. Allergy 유발특성을 보면, RV-1은 초기유발강도가 강하고 지속적이어서 회복속도도 늦었다. RV-2는 초기유발강도는 약하지만 회복속도는 늦었다. 반면에 RV-3는 초기유발강도는 비교적 약했지만, 감작 후 48시간 째에 가장 강하게 유발되었고, 회복속도가 빨라서 72시간이 경과하였을 때에는 거의 대조군 수준으로 회복되었다. 따라서 allergy유발에 가장 큰 영향을 미치는 urushiol은 RV-1이었으며, RV-1의 함량이 가장 많은 중국산이allergy의 유발강도가 가장 강하였고 회복속도도 늦음을 알 수 있었다.
옻 allergy 유발물질을 분리하고 그 특성을 연구하기 위하여 국내산, 중국산 및 일본산 생 옻을 사용하였다. 옻의 crude extracts로부터 crude urushiol을 정제하고, 다시 allergy 유발물질로 알려져 있는 3종의 urushiol congeners를 분리하였다. 분리한 urushiol congeners는 RV-1 였으며, 전체의 80%에 해당하였다. RV-1의 함량은 중국산에서 70.07%로 가장 많았으며, 일본산에서 62.38%로 가장 적었다. 반면에 RV-2는 일본산은 9.25%로서 국내산과 중국산의 4.28 및 3.09%에 비하여 약 2$\sim$3 배정도 많았다. Allergy 유발특성을 보면, RV-1은 초기유발강도가 강하고 지속적이어서 회복속도도 늦었다. RV-2는 초기유발강도는 약하지만 회복속도는 늦었다. 반면에 RV-3는 초기유발강도는 비교적 약했지만, 감작 후 48시간 째에 가장 강하게 유발되었고, 회복속도가 빨라서 72시간이 경과하였을 때에는 거의 대조군 수준으로 회복되었다. 따라서 allergy유발에 가장 큰 영향을 미치는 urushiol은 RV-1이었으며, RV-1의 함량이 가장 많은 중국산이allergy의 유발강도가 가장 강하였고 회복속도도 늦음을 알 수 있었다.
We purified crude urushiol from natural lacquer produced in Korea, China, and Japan and then isolated several urushiol congeners known to induce allergic reactions. The 3 major kinds of urushiol congeners that were isolated and purified were RV-l (C$_{21}$H$_{34}$O$_2$
We purified crude urushiol from natural lacquer produced in Korea, China, and Japan and then isolated several urushiol congeners known to induce allergic reactions. The 3 major kinds of urushiol congeners that were isolated and purified were RV-l (C$_{21}$H$_{34}$O$_2$, M.W. 314.462), RV-2 ((C$_{21}$H$_{32}$O$_2$, M.W. 316.240) and RV-3 ((C$_{23}$H$_{34}$O$_2$, M.W. 342.515), occupying 80% of total crude urushiol. The content of RV-l was the highest in natural lacquer from China at 70.07%, and was the lowest in that from Japan at 62.38%. However, the content of RV-2 in natural lacquer from Japan was 9.25%, 2$\sim$3 times higher than those from Korea (4.28%) and China (3.09%). As an allergy inducing character, RV-l had strong inducing power and durability in the primary stage, showing slow recovery. RV-2 had weak power in the primary stage and also showed slow recovery. Although RV-3 had comparatively weak power at the primary stage, it induced the strongest allergy contact dermatitis after 48 hr. However, it recovered to nearly the same level as control group 72 hr after sensitization time. Accordingly, we found out that RV-1 is the most influential of urushiol congeners in inducing allergic reactions, natural lacquer from China having the most inducible strength and slowest recovery compared to those from Korea and Japan.
We purified crude urushiol from natural lacquer produced in Korea, China, and Japan and then isolated several urushiol congeners known to induce allergic reactions. The 3 major kinds of urushiol congeners that were isolated and purified were RV-l (C$_{21}$H$_{34}$O$_2$, M.W. 314.462), RV-2 ((C$_{21}$H$_{32}$O$_2$, M.W. 316.240) and RV-3 ((C$_{23}$H$_{34}$O$_2$, M.W. 342.515), occupying 80% of total crude urushiol. The content of RV-l was the highest in natural lacquer from China at 70.07%, and was the lowest in that from Japan at 62.38%. However, the content of RV-2 in natural lacquer from Japan was 9.25%, 2$\sim$3 times higher than those from Korea (4.28%) and China (3.09%). As an allergy inducing character, RV-l had strong inducing power and durability in the primary stage, showing slow recovery. RV-2 had weak power in the primary stage and also showed slow recovery. Although RV-3 had comparatively weak power at the primary stage, it induced the strongest allergy contact dermatitis after 48 hr. However, it recovered to nearly the same level as control group 72 hr after sensitization time. Accordingly, we found out that RV-1 is the most influential of urushiol congeners in inducing allergic reactions, natural lacquer from China having the most inducible strength and slowest recovery compared to those from Korea and Japan.
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문제 정의
Urushi lacquer is possibly the most suitable and natural alternation. Therefore, this research aims to provide the groundwork in breeding the low-allergenic Rhus vanidHva Stokes and in developing urushi lacquer. In this research, lacquer for China, Japan and Korea were used in comparison.
제안 방법
The CDCOCD3 and CD3OD for NMR analysisz were Aldrich's products, and DNCB (2z4-dinitrochlorobenzene) of Sigma products was used as allergen of hypersensitive reaction against mouse. Used instruments for this study are as follows; rotary vacuum evaporator (Biichiz Germany) and fraction collector (Eyela, Japan) were used for isolation and purification, and HPLC (Shimadzu LC-10AD, Japan) for determining urushiol congeners contents. NMR (Brukerz Germany) and El/Mass (JMSZ DX300z Japan) were used to identify chemical structure of allergy inducing materials.
대상 데이터
BALB/c mice, four-weeks of age (20±2 g), were purchased from Hyochang science co. and were used in this research after adaptation in the breeding room for two-weeks. We supplied water fredy and conditions of breeding room were kept at 22±2℃ with controlled 12-hr-light / 12-hr-dark cyde during the all experimental period.
The lacquers used in this research, Korea (Weonju products), China (Sumseo products), and Japan (Iwate products), were provided from Jinju National Universit矿s Department of Interior Materials Engineering. Particularly, the Weonju products were used to isolate the allergy inducing materials.
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