[논문]4주간 흰쥐의 목초액 섭취와 운동에 따른 혈중지질과 MDA, SOD 활성 차이

강종석; 김세현; 김판기; 이동운; 류승필

doi:10.5352/jls.2009.19.9.1190

4주간 흰쥐의 목초액 섭취와 운동에 따른 혈중지질과 MDA, SOD 활성 차이
Differences of Wood Vinegar Ingestion and Exercise Training on Blood Lipids, MDA, and SOD Activities in Rats 원문보기

생명과학회지 = Journal of life science, v.19 no.9 = no.113, 2009년, pp.1190 - 1199

강종석 (경북대학교 생태환경대학 레저스포츠학과) , 김세현 (국립산림과학원 산림유전자부) , 김판기 (경북대학교 생태환경대학 생태환경시스템학부) , 이동운 (경북대학교 생태환경대학 생태자원응용학부) , 류승필 (경북대학교 생태환경대학 레저스포츠학과)

초록
AI-Helper

본 연구는 운동훈련과 목초액의 장기섭취가 혈중 지질성분 및 항산화활성에 미치는 영향을 살펴보고자 하였다. 28마리의 Sprague Dawley 수컷 쥐를 이용하여 대조군(CON), 운동군(EXE), 목초액섭취군(VIN), 그리고 목초액과 운동군을 병행한 군(VINEXE)으로 각각 7마리 씩 구분하였다. 식이는 고지방식이(40%)를 기본으로 하였으며, 운동군은 4주간의 트레드밀 달리기하였으며, 목초액은 경구투여하였다. 목초액을 섭취하지 않는 집단에게는 동일한 양의 증류수를 경구투여하여 동일한 스트레스를 유지할 수 있도록 하였다. 체중의 변화는 운동 후반기에 CON에 비하여 VINEXE이 유의하게 억제되는 것으로 나타났다(p<0.05). 지방량의 변화는 VIN 및 EXE군에서 유의한 상승의 억제가 나타났으며(p<0.05), VINEXE 역시 동일한 결과가 나타났다(p<0.05). 혈액성분 중 글루코스와 암모니아는 CON에 비하여 다른 집단에서 유의하게 낮은 것으로 나타났으며(p<0.05). 혈중 지질 중 TC, LDL-C는 CON에 비하여 다른 집단이 유의하게 낮은 것으로 나타났으며, VINEXE의 유의한 상승효과가 나타났다(p<0.05). HDL-C는 EXE와 VINEXE군에서 유의하게 개선되는 것으로 나타났다(p<0.05). MDA 함량은 각각의 집단간 모두 유의한 변화를 보였으며(p<0.05), SOD 활성은 VIN과 VINEXE가 다른 집단에 비하여 유의하게 높은 것으로 나타났다(p<0.05). 이상과 같은 결과를 볼 때 4주간의 운동을 하는 동안 목초액 섭취는 체중 증가 억제 및 혈중 지질성분의 개선, 그리고 지질과산화 억제로 나타나며, 건강 증진에 도움을 줄 수 있을 것이라는 점을 시사하고 있다.

Abstract ▼ AI-Helper

The purpose of this study is to identify the effects of exercise training and oak tree wood vinegar ingestion on the blood lipids and antioxidant activities of rats. The subjects were 28 Sprague Dawley male rats, and they were assigned into four groups (n=7, respectively): the control group (CON), the exercise group (EXE), the vinegar ingestion group (VIN), and the vinegar ingestion and exercise training group (VINEXE). The diet was based on high fat and oral administration of oak tree wood vinegar. The rats that were not given oak tree wood vinegar were given the same amount of distilled water orally in order to maintain the same level of stress. They were exercise trained on motor-driven treadmills during a four-week session. Weight changes in the VINEXE were significantly inhibited in the later period of exercise, when compared to the CON (p<0.05). Fat increase was significantly suppressed in VIN and EXE (p<0.05), and a synergistic effect was discovered in the VINEXE (p<0.05). Glucose and ammonia levels were significantly reduced in the EXE, VIN, and VINEXE compared to the CON (p<0.05). In blood lipids, TC and LDL-C were significantly enhanced in the EXE, VIN, and VINEXE compared to the CON (p<0.05), while HDL-C was significantly improved in the EXE and VINEXE (p<0.05). Liver MDA contents showed significant changes in each group (p<0.05), and SOD activities were significantly enhanced in the VIN and the VINEXE when compared to other groups (p<0.05). Therefore, oak tree wood vinegar ingestion with exercise training for four weeks may result in inhibition of weight gain, improvement of blood lipids, and inhibition of lipid peroxidation, contributing to health promotion.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

가설 설정

Especially, there is few research of related to the exercise training and wood vinegar ingestion. Therefore, in this study, we examined the influence of oak tree wood vinegar as a natural food on blood lipids concentration and the effect of inhibition of lipid peroxidation that could be induced by exercise.

제안 방법

on the blood lipids and lipid peroxides. For doing this, 28 SD male rats were selected as the subjects that were orally administered of oak tree wood vinegar with treadmill running training and high fat diet for two weeks and sacrificed for collecting data.
Water was replenished when the bottles were half empty and 200 ml of water was basically provided at a time. In this study, the rats underwent restrict diet by 10 g of feed in the morning and in the afternoon for providing identical calories. The high-fat diet used in this study (C: 43%z F: 40%z P: 17%) was made the basis of the tables of food composition (AIN-76).
The exercise training was performed five times per week, for four weeks. The intensity of exercise, 10 m/min training was provided in the beginning of the experiment at 8% incline and 27 m/min training was provided in the 4th week, under the principle of incremental exercise program.

대상 데이터

For this study, 28 male ratsz aged five-week of Sprague- Dawley (SD) were purchased from Hyochang Science (Korea) as the subjects. The rats of the same age and the weight from 120 to 140 g were purchased, and preliminary breeding had been carried out for two weeks before group classification.
The rats underwent exercise training on the motor-driven treadmill for small animals (10 lanes with 12 cm in breadth, 10 cm in height, and 1 m in length). The exercise training was performed five times per week, for four weeks.
The rats were divided into the control group (CON), the exercise training group (EXE), the vinegar ingestion group (VIN)Z and the vinegar ingestion and exercise training group (VINEXE). The vinegar used in this study was the 100% oak tree wood vinegar made by K Company (Korea), and the test solution was made by mixture of the 100% undiluted solution with distilled water to make 35% solution, which then was diluted to be 50% in concentration. Oak tree wood vinegar was orally administered by 1 ml one hour before each exercise for four weeks between the beginning and the end of exercise training by using of zonde for oral administration.

데이터처리

z USA) for Windowsz and the data were represented by mean+standard error of mean (SEM). The one-way ANOVA was used in order to compare all the variables of each groupz and tukey test was performed as post-hoc test when there was significant difference. The sig- nificance level of all the differences was p<0.

이론/모형

MDA was analyzed by using the method presented by Saitoh [33]. 0.
In this study, the rats underwent restrict diet by 10 g of feed in the morning and in the afternoon for providing identical calories. The high-fat diet used in this study (C: 43%z F: 40%z P: 17%) was made the basis of the tables of food composition (AIN-76). The rats were divided into the control group (CON), the exercise training group (EXE), the vinegar ingestion group (VIN)Z and the vinegar ingestion and exercise training group (VINEXE).

성능/효과

As for the amount of weight gain, the VIN and the VINEXE showed significant inhibition of gain when com- pared to the CON and the EXE (p<0.05)z and that of the VINEXE showed the lowest gain amount (p<0.05). Given that no significant difference was expressed among the whole groups in food efficiencyz there might be no effects by meal.
Also, TC and LDL-C were shown to decrease by administration of the vinegar. However, there were no significant differences in TG and TC between the VIN and VINEXE, and the single effect of oak tree wood vinegar was more considerable than the effect of exercise. It may be acetic acid that affects the decline in blood lipid concentration by administration of oak tree wood vinegar.
Kim [18] reported an experiment of toxicity of refined oak tree wood vinegar in which ICR mice were administered with the vinegar for four weeks with different amounts. In the study, mice that were administered with 1.0 g/kg had been significantly reduced of their weight gain since the 21st dayz and 5.0 g/kg showed significant difference on the 28th day. In the present study, no statistically significant difference was shown among the all groups during the whole period, but on the 25th day statistically significant difference was expressed between the CON and the VINEXE, a result that was similar to that of Kim [18].

참고문헌 (37)

Bogani, P., C. Galli, M. Villa, and F. Visioli. 2006. Postprandial anti-inflammatory and antioxidant effects of extra virgin oil. Atherosclerosis 190, 181-186

상세보기
Chyla, M. and 1. Vrzgula. 1978. Intensity of acid-base reactions of the blood and cerebrospinal fluid in sheep after intraluminal stress induced by urea and acetic acid. Veterinary Medicine (Praha) 23, 9-18
Cho, W. K. and J. H. Choi. 2007a. Effect of Pyroligneous Liquor on oxygen radicals and their scavenger enzymes in liver of CD rats. Kor. J. Nutr. 40, 111-117
Cho, W. K. and J. H. Choi. 2007b. Effect of Pyroligneous Liquor on lipid metabolism in serum of CD rats. Kor. J. Nutr. 40, 24-30
Crabtree, B., M. J. Gordon, and S. 1. Christie. 1990. Measurement of the rates of acetyl-CoA hydrolysis and synthesis from acetate in rat hepatocytes and the role of these fluxes in substrate cycling. Biochem. J. 270, 219-225

상세보기
Friedewald, W. T., R. I. Levy, and D. S. Fredricson. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without was of the preparative ultracentrifuge. Clin. Chem. 18, 499-506
Fushimi, T. and Y. Sato. 2005. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br. J. Nutr. 94, 714-719

상세보기
Fushimi, T., K. Suruga, Y. Oshima, M. Fukiharu, Y. Tsukamoto, and T. Goda. 2006. Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet. Br. J. Nutr. 95, 916-924

상세보기
Fushimi, T., K. Tayama, M. Fukaya, K. Kitakoshi, N. Nakai, Y. Tsukamoto, and Y. Sato. 2001. Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats. J. Nutr. 131, 1973-1977

상세보기
Fushimi, T., K. Tayama, M. Fukaya, K. Kitakoshi, N. Nakai, Y. Tsukamoto, and Y. Sato. 2002. The efficacy of acetic acid for glycogen repletion in rat skeletal muscle after exercise. Int. J. Sports Med. 23, 218-222

상세보기
Jeong, c. H. and K. H. Shim. 2002. Nitrite-scavenging and antioxidant of Wood Vinegar. Kor. J. Food Preserv. 9, 351-355
Jodai, S., S. Yano, and T. Uehara. 1989. Component of wood vinegar liquors and their smoke flavors. Mokuzai Gakkaishi 35, 555-563
Johnston, C. S. 2005. Strategies for healthy weight loss: from vitamin C to the glycemic response. J. Am. ColI. Nutr. 24, 158-165

상세보기
Johnston, C. S., and A. J. Buller. 2005. Vinegar and peanut products as complementary foods to reduce postprandial glycemia. J. Am. Diet. Assoc. 105, 1939-1942

상세보기
Johnston, C. S., C. M. Kim, and A. J. Buller. 2004. Vinegar improves insulin sensitivity to a high-carbohydrate meal in subjects with insulin resistance or type 2 diabetes. Diabetes Care, 27, 281-282

상세보기
Jung, I. S., Y. J. Kim, S. W. Gal, and Y. J. Choi. 2007. Antimicrobial and antioxidant activities and inhibition of nitric oxide synthesis of oak wood vinegar. J. Life Sci. 17, 105-109
Kim, D. H, J. S. Choi, J. H Choo, H C. Song, E. O. Lee, I. C. Kang, J. W. Choi, and S. H Kim. 200la. Study on immunomodulatory and antitumor activity of holyessing. Kor. J. Orient. Med. Physiol. & Pathol. 15, 881-886
Kim, P. G. 1996. Subacute toxicity study of refined wood vinegar. Bull. Nature Sci. Yongin Univ. 1, 35-49
Kim, S. H., T. W. Kwon, Y. S. Lee, M. G. Choung, and G. S. Moon. 2005. A major anti oxidative components and comparison of antioxidative activities in black soybean. Kor. J. Food Sci. Technol. 37, 73-77
Kim, Y. H, S. K. Kim, K. S. Kim, and Y. H Lee. 2001b. Composition of constituents of commercial wood vinegar liquor in Korea. J. Kor. Soc. Agri. Chem. Biotechnol. 44, 262-268
Lee, J. S., S. M. Jeon, E. M. Park, T. 1. Huh, O. S. Kwon, M. K. Lee, and M. S. Choi. 2003. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats. J. Med. Food 6, 183-191

상세보기
Lee, K. M., G. T. Jeong, and D. H Park. 2004. Study of antimicrobial and DPPH radical scavenger activity of wood vinegar. Kor. J. Biotechnol. 19, 381-384
Lee, S. M., S. B. Lee, and M. C. Lee. 2006. The effect of phellinus igmanus extract administration on exercise- performing ability, the level of muscle fatigue and antioxidant system. Kor. J. Exer. Nutr. 10, 173-179
Lo, S., J. c. RusseL and A W. Taylor. 1970. Determination of glycogen in small tissues. J. Appl. Physiol. 28, 234-236
Lowry, O. H, N. J. Rosenbrough, A l. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. BioI. Chem. 193, 265-275
Marklund, S. L., P. M. Andersen, L. Forsgren, P. Nilsson, P. I. Ohlsson, G. Wikander, and A Oberg. 1997. Normal binding and reactivity of copper in mutant superoxide dismutase isolated from amyotrophic lateral sclerosis patients. J. Neurochem. 69, 675-681

상세보기
McGarry, J. D. and N. F. Brown. 1997. The mitochondrial carnitine palmitoyltransferase system. From concept to molecular analysis. Eur. J. Biochem. 244, 1-14

상세보기
Ogawa, N., H. Satsu, H. Watanabe, M. Fukaya, Y. Tsukamoto, Y. Miyamoto, and M. Shimizu. 2000. Acetic acid suppresses the increase in disaccharidase activity that occurs during culture of caco-2 cells. J. Nutr. 130, 507-513

상세보기
Oh, D. Y., and S. J. Chung. 2007. Effect of antioxidant activities of water-soluble chitosan in oak smoke flavoring. J. Chitin & Chitosan 12, 228-233
O'Keefe, J. H, N. M. Gheewala, and J. O. O'Keefe. 2008. Dietary strategies for improving post-prandial glucose, lipids, inflammation, and cardiovascular health. J. Am. ColI. Cardiol. 51, 249-255.

상세보기
Ostman, E., Y. Granfeldt, L. Persson, and I. Bjorck. 2005. Vinegar supplementation lowers glucose and insulin responses and increases satiety after a bread meal in healthy subjects. Eur. J. Clin. Nutr. 59, 983-988

상세보기
Pomare, E. W., W. J. Branch, and J. H. Cummings. 1985. Carbohydrate fermentation in the human colon and its relation to acetate concentrations in venous blood. J. Clin. Invest. 75, 1448-1454

상세보기
Saitoh, K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 90, 37-43

상세보기
Shen, Y., S. Chen, and C. Wang. 2007. Contribution of tomato phenolics to antioxidation and down-regulation of blood lipids. J. Agri. Food Chem. 55, 6475-6481

상세보기
Tagliazucchi, D. 2005. Vinegar poluphenol increase the proteolysis and decrease the oxidative stress during digestion by pepsin, Book of Abstract, International Symposium of Vinegar and Acetic acid bacteria, Reggio Emilia, Italy, pp. 83, www.vinegar2005.com
Van Schaftingen, E., and H G. Hers. 1986. Purification and properties of phosphofructokinase 2/ fructose 2,6-bisphosphatase from chicken liver and from pigeon muscle. Eur. J. Biochem. 159, 359-365

상세보기
Vince, A, M. Killingley, and O. M. Wrong. 1978. Effect of lactulose on ammonia production in a fecal incubation system. Gastroenterology 74, 544-549

상세보기

저자의 다른 논문 :

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증