[논문]혈관내피세포에서 토복령(土茯苓)의 항산화 및 항염증 효과

이창현; 이효승; 김재은; 허숙경; 차창민; 원찬욱; 박선동

doi:10.6116/kjh.2009.24.3.039

혈관내피세포에서 토복령(土茯苓)의 항산화 및 항염증 효과
Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.24 no.3, 2009년, pp.39 - 50

이창현 (동국대학교 한의과대학 방제학교실) , 이효승 (심혈관계질환 천연물 연구개발센터) , 김재은 (심혈관계질환 천연물 연구개발센터) , 허숙경 (동국대학교 한의과대학 방제학교실) , 차창민 (동국대학교 한의과대학 방제학교실) , 원찬욱 (동국대학교 한의과대학 방제학교실) , 박선동 (심혈관계질환 천연물 연구개발센터)

Abstract ▼ AI-Helper

Objectives : Smilacis glabrae rhizoma (SG) has been traditionally used as a herbal medication of musculoskeletal disorders like arthritis, pain, convulsions, and syphilis in traditional Korean medicine. This study was investigated anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell (HUVEC). Methods : SG extract prepared with methanol, and then fractionated with hexane, dichloromethane, ethylacetate, n-butanol and water. Inhibitory effect of SG onto free radical generation was determined by measuring DPPH, superoxide anions and nitric oxide scavenging activities in vitro. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymethoxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Intracelluar oxidation was analysed by DCF-DA assay. The nitric oxide (NO) production was measured by Griess reagent system. The levels of ICAM-1 and VCAM-1 expression were confirmed by western blot. And proinflammatory cytokines were measured by ELISA kit. Results : Our results indicated that fractionated extracts, especially ethyl acetate (EA) extract, significantly inhibited free radical generation, the TNF-$\alpha$-induced intracellular oxidation. Furthermore, the EA extract protected TNF-$\alpha$-induced adhesion to THP-1, expression of adhesion molecules accompanied by an attenuation of IL-6 and IL-8 formation in HUVEC. Conclusions : These results indicate that EA extract of SG have potential as an agent of atherosclerosis and other chronic inflammatory diseases including diabetes, hypertension, and arthritis.

주제어

질의응답

핵심어	질문	논문에서 추출한 답변
	동맥경화을 일으키는 중요한 위험요소는?	동맥경화를 일으키는 중요한 위험 요소로 알려진 것은 고혈압, 고지혈증, 흡연 등을 들 수 있으며, 이 외에도 지방과 염분의 과다섭취, 과식, 운동부족, 스트레스, 당뇨병 등이 위험인자로 작용한다. 이러한 위험 인자들이 동맥경화를 유발시키기까지의 분자 생물학적인 발생기전은 2000년까지도 잘 알려지지 않은 상태였지만, 최근에 많은 연구 결과들이 나오면서 원인에 대한 분자 생물학적인 기전들이 밝혀지고 있고 치료제에 대한 연구도 활발하게 진행되고 있다2).
	죽상동맥경화증으로 인해 유발하는 것은?	죽상동맥경화증 (atherosclerosis)은 죽상판 또는 섬유 지방판을 형성하여 혈관의 내강을 좁히고, 중막을 약화시켜 각종 합병증을 유발한다. 죽상경화증은 특히 대동맥, 심장동맥 및 대뇌의 동맥에 흔해서 심장근육경색, 대뇌경색 및 대동맥 동맥류 등의 합병증을 초래한다. 뿐만 아니라 동맥혈류를 감소시켜서 팔다리의 괴저, 장간막 혈관폐쇄로 인한 장경색증, 급성 심장사, 만성 허혈성 심장질환, 뇌혈관장애질환 등을 유발한다1).

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