To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, ...
To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 ${\mu}/g$, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The $IC_{50}$ values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 ${\mu}/g$, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The $IC_{50}$ values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
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제안 방법
The absorbance at 562 nm was determined spectrophotometrically. Ethylendiaminetetraacetate (EDTA) was used as positive control and chelating activity on ferrous ion was calculated using the same equation given in DPPH radical scavenging activity, with ACOntroi as the absorbance of the blank without extract or EDTA and Asampie as the absorbance in the presence of the culture broth or EDTA.
In this study, the antioxidant activities of the culture broth from submerged culture of T. fuciformis were investigated in vitro and evaluated by scavenging abilities on DPPH and ABTS, the reducing power, and the 아lelating ability on ferrous ions.
이론/모형
The total flavonol content was estimated using the p-dimethylaminocinnamaldehyde (DMACA) method [20]. Culture broth (0.
Total polyphenol content was determined using the Folin-Ciocalteu method [18], adapted to a micro scale. In a 15-.
성능/효과
These results suggest that pharmacological functions of T. fuciformis are due to, at least partially, their protective effects against oxidation and that it is a promising way to use the culture broth of the fungus for the antioxidant activity to reduce the human demands on the natural resources of T. fuciformis, an endangered species.
후속연구
Since polyphenolic compounds might be a possible class of antioxidant in this mushroom, future work on the isolation and structural characterization of the active components is needed. Also, the antioxidant activity of these components, in regard to the mechanisms for radical scavenging and protection against lipid peroxidation, will be the primary objective of further investigation.
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