To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, ...
To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 ${\mu}/g$, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The $IC_{50}$ values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
To investigate antioxidant activity of the culture broth from submerged culture of Tremella fuciformis, we preferentially analyzed the chemical composition of culture broth, which was mainly composed of carbohydrate (296.39 mg/g) and protein (9.24 mg/g), respectively. Also, contents of polyphenols, flavonoids and flavonols were 16.63 mg/g, 9.19 mg/g and 83.74 ${\mu}/g$, respectively. Next, we examined the scavenging abilities on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radical, the reducing power, and chelating ability on ferrous ions. All antioxidant activities of the culture broth were increased in proportion to its concentrations. The $IC_{50}$ values were in order as follows ABTS radical scavenging activity < OPPH radical scavenging activity < chelating power. Accordingly, these results suggest that pharmacological function of T. fuciformis might be due to, at least partially, their protective effects against oxidation and the culture broth of T. fuciformis was free radical inhibitors or scavengers at low concentration, involving possibly in termination of free radical reaction as primary antioxidants.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
In this study, the antioxidant activities of the culture broth from submerged culture of T. fuciformis were investigated in vitro and evaluated by scavenging abilities on DPPH and ABTS, the reducing power, and the 아lelating ability on ferrous ions.
The absorbance at 562 nm was determined spectrophotometrically. Ethylendiaminetetraacetate (EDTA) was used as positive control and chelating activity on ferrous ion was calculated using the same equation given in DPPH radical scavenging activity, with ACOntroi as the absorbance of the blank without extract or EDTA and Asampie as the absorbance in the presence of the culture broth or EDTA.
이론/모형
Total polyphenol content was determined using the Folin-Ciocalteu method [18], adapted to a micro scale. In a 15-.
The total flavonol content was estimated using the p-dimethylaminocinnamaldehyde (DMACA) method [20]. Culture broth (0.
성능/효과
These results suggest that pharmacological functions of T. fuciformis are due to, at least partially, their protective effects against oxidation and that it is a promising way to use the culture broth of the fungus for the antioxidant activity to reduce the human demands on the natural resources of T. fuciformis, an endangered species.
후속연구
Since polyphenolic compounds might be a possible class of antioxidant in this mushroom, future work on the isolation and structural characterization of the active components is needed. Also, the antioxidant activity of these components, in regard to the mechanisms for radical scavenging and protection against lipid peroxidation, will be the primary objective of further investigation.
참고문헌 (41)
Coyle, J. T. and P. Puttfarcken (1993) Stress, glutamate, and neurodegenerative disorders. Science. 262: 689-695.
Diolock, A. T., J. L. Charleux, G. Crozier-Willi, F. J. Kok, C. Rice-Evans, M. Roberfroid, W. Stahl, and J. Vina-Ribes (1998) Functional food science and defense against reactive oxidative species. Br. J. Nutr. 80: 77-112.
Saito, M., H. Sakagami, and S. Fujisawa. (2003) Cytotoxicity and apoptosis induction by butylated hydroxyanisole (BRA) and butylated hydroxytoluene (BHT). Anticancer Res. 23: 4693-4701.
Stefanidou, M., G. Alevisopoulos, A. Chatziioannou, and A. Kouteslinis (2003) Assessing food additive toxicity using a cell model. Vet. Hum. Toxicol. 45: 103-105.
Lee, B. C., J. T. Bae, H. B. Pyo, T. B. Choe, S. W. Kim, H. J. Hwang, and J. W. Yun (2003) Biological activities of the polysaccharides produced from submerged culture of the edible basidiomycete Grifola frondosa. Enzyme Microb. Technol. 32: 574-581.
Lee, I. H., R. L. Huang, C. T. Chen, H. C. Chen, W. C. Hsu, and M. K. Lu (2002) Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol. Lett. 209: 63-67.
Liu, F., V. E. C. Ooi, and S. T. Chang (1997) Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60: 763-771.
Tslapali, E., S. Whaley, J. Kalbfleisch, H. E. Ensley, I. W. Browder, and D. L. Williams (2001) Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity. Free Radic. Biol. Med. 30: 393-402.
Cheung, P. C. K. (1996) The hypercholesterolemic effect of two edible mushrooms: Auricularia auricula (tree-ear) and Tremella fuciformis (white jelly-leaf) in hypercholesterolemic rats. Nutr. Res. 16: 1721-1725.
Gao, Q., R. Seljejid, H. Chen, and R. Jiang (1996) Characterisation of acidic heteroglycans from Tremella fuciformis Berk with cytokine stimulating activity. Carbohydr. Res. 288: 135-142.
Reshetnikov, S. R., S. P. Wasser, I. Duckman, and K. Tsukor (2000) Medicinal value of the geuns Tremella Pers (Heterobasidiomycetes). Int. J. Med. Mushrooms. 2: 169-193.
Waterman, P. G. and S. Mole (1994) Analysis of Polyphenolic Plant Metabolites. p. 83. Blackwell Scientific Publ, Oxford.
Woisky, R. G. and A. Salatino (1998) Analysis of propolis: some parameters and procedures for chemical quality control. J. Apicult. Res. 37: 99-105.
Li, Y. G., G. Tanner, and P. Larkin (1996) The DMACA-HCl protocol and the threshold proantho-cyanidin content for bloat safety in forage legumes. J. Sci. Food Agric. 70: 89-101.
Omran, H., H. Buchenhuskes, B. Zapo, and K. Gierscher (1989) Technical enzymes for the liquefaction of white cabbage and sauerkraut. Food Biotech. 3: 59-63.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye biding. Anal. Biochem. 72: 248-254.
Cheung, L. M., P. C. K. Cheung, and V. E. C. Ooi (2003) Antioxidant activity and total polyphenolics of edible mushroom extracts. Food Chem. 81: 249-255.
Re, R., N. Pellegrini, A. Proteggente, A. Pannala, and M. Yang (1999) Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237.
Forni, L. G., V. O. Mora-Arellano, J. E. Packer, and R. L. Willson (1986) Nitrogen dioxide and related free radicals: electron-transfer reactions with organic compounds in solutions containing nitrite or nitrate. J. Chem. Soc. Perkin Trans. 2: 1-6.
Decker, E. A. and B. Welch (1990) Role of ferritin as a lipid oxidation catalyst in muscle food. J. Agric. Food Chem. 38: 674-677.
Wada, T., Y. Hayashi, and H. Shibata (1996) Asiaticusin A and B, novel prenylated polyphenolics from Boletinus asiaticus and B. paluster (Boletaceae) fungi. Biosci. Biotech. Bioch. 60: 120-121.
Huang, L. C. (2000) Antioxidant Properties and Polysaccharide Composition Analysis of Antrodia camphorate and Agaricus blazei. Master's Thesis. National Chung-Hsing University, Taichung, Taiwan.
Mokbel, M. S. and F. Hashinaga (2006) Evaluation of the antioxidant activity of extracts from buntan (Citrus grandis Osbeck) fruit tissues. Food Chem. 94: 529-534.
Shimada, K., K. Fujikawa, K. Yahara, and T. Nakamura (1992) Antioxidative properties of xantan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agri. Food Chem. 40: 945-948.
Ferreira, I. C., F. R. Baptista, P. Vilas-Boas and L. Barros (2007) Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 100: 1511-1516.
Mau, J. L., S. Y. Tsai, Y. H. Tseng, and S. J. Huang (2005) Antioxidant properties of hot water extracts from Ganoderma tsugae Murrill. LWT-Food Sci. Technol. 38: 589-597.
Huang, G. W. (2003) Taste Quality and Antioxidant and Antimutagenic Properties of Pleutotus citrinopileatus. Master's Thesis. National Chung-Hsing University, Taichung, Taiwan.
Huang, D. J., B. X. Ou, and R. L. Prior (2005) The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53: 1841-1856.
Murcia, A. M., M. Martinez-Tome, A. M. Jimenez, A. M. Vera, M. Honrubia, and P. Parras (2002) Antioxidant activity of edible fungi (truffles and mushrooms): losses during industrial processing. J. Food Prot. 65: 1614-1622.
Mattila, P., P. S. Vaananen, K. Kongo, H. Aro, and T. Jalava (2002) Basic composition and amino acid contents of mushrooms cultivated in Finland. J. Agric. Food Chem. 50: 6419-6422.
Endo, A. (1998) Chemistry, biochemistry, and pharmacology of HMO CoA reductase inhibitors. Klin. Wochenschr. 66: 421-427.
Jose, N. and K. K. Janardhanan (2000) Antioxidant and antitumour activity of Pleurotus florida. Curr. Sci. 79: 941-943.
Lin, H. C. (1999) Evaluation of Taste Quality and Antioxidant Properties of Edible Mushrooms. Master's Thesis. National Chung-Hsing University, Taichung, Taiwan.
이 논문을 인용한 문헌
저자의 다른 논문 :
활용도 분석정보
상세보기
다운로드
내보내기
활용도 Top5 논문
해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다. 더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.