Angelicae gigas Nakai has been used as a traditional medicine as well as an edible vegetable in South Korea. In this study, the total phenolic and flavonoid content and antioxidants of A. gigas Nakai leaves were examined in vitro via hydroxyl-radical-scavenging activity, reducing power activity, met...
Angelicae gigas Nakai has been used as a traditional medicine as well as an edible vegetable in South Korea. In this study, the total phenolic and flavonoid content and antioxidants of A. gigas Nakai leaves were examined in vitro via hydroxyl-radical-scavenging activity, reducing power activity, metal chelating assay, and DPPH-free -radical-scavenging assay. Among all the extracts from A. gigas Nakai leaves, the ethanol extract showed the strongest effects in all of the assays. The $EC_{50}$ values for the DPPH-radical-scavenging activities of ethanol, methanol, and water extracts were 31.47, 42.14, and $58.47{\mu}g/mL$, respectively. Among the extracts from A. gigas Kakai leaves, the ethanol extract had the highest levels of total phenolics ($7.84{\pm}1.46$ mg TAN/g) and total flavonoids ($4.23{\pm}0.03$ mg QE/g), which correlated strongly with the individual phenolic-compound (p-hydroxybenzoic acid, vanillin, and trans-ferulic acid) contents. The ethanol extract also showed stronger antioxidant activity than tocopherol in hydroxyl- radical-scavenging activity assay. These results indicate that the ethanol extract of A. gigas Kakai leaves possesses significant antioxidant properties, which suggests its great potential as a functional-food ingredient in the food industry.
Angelicae gigas Nakai has been used as a traditional medicine as well as an edible vegetable in South Korea. In this study, the total phenolic and flavonoid content and antioxidants of A. gigas Nakai leaves were examined in vitro via hydroxyl-radical-scavenging activity, reducing power activity, metal chelating assay, and DPPH-free -radical-scavenging assay. Among all the extracts from A. gigas Nakai leaves, the ethanol extract showed the strongest effects in all of the assays. The $EC_{50}$ values for the DPPH-radical-scavenging activities of ethanol, methanol, and water extracts were 31.47, 42.14, and $58.47{\mu}g/mL$, respectively. Among the extracts from A. gigas Kakai leaves, the ethanol extract had the highest levels of total phenolics ($7.84{\pm}1.46$ mg TAN/g) and total flavonoids ($4.23{\pm}0.03$ mg QE/g), which correlated strongly with the individual phenolic-compound (p-hydroxybenzoic acid, vanillin, and trans-ferulic acid) contents. The ethanol extract also showed stronger antioxidant activity than tocopherol in hydroxyl- radical-scavenging activity assay. These results indicate that the ethanol extract of A. gigas Kakai leaves possesses significant antioxidant properties, which suggests its great potential as a functional-food ingredient in the food industry.
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제안 방법
Studies of this plant have primarily been conducted using root samples; thus, little information is available regarding the leaves of Angelicae gigas, which are consumed as a vegetable in Korea (17). To investigate the potential antioxidant properties of Angelicae gigas leaf, we utilized a number of in vitro assay systems, including hydroxyl radical scavenging assay, metal chelating assay, DPPH free radical scavenging assay, phenolic acids, total phenolic and total flavonoid measurement.
대상 데이터
1,1-Diphenyl-2-picryhydrazyl (DPPH), α-tocopherol, 3-(2- pyridyl)-5,6-bis (4-phenyl-sulfonic acid)-1,2,4-triazine (ferrozine), trichloroacetic acid (TCA), sulfanilamide, naphthy-lethlenediamine dihydrochloride, tannic acid, Folin-Ciocalteu’s, BHT, and LPS (E. coli 0111:B4) were purchased from Sigma (St. Louis, MO, USA).
Leaves of Angelicae gigas collected in September 2008 in PyeongChang, Korea, were dried in the shade at room temperature and then powdered. Two hundred grams of the leaf powder were extracted separately with 80% methanol, 80% ethanol at 70℃ and with hot water for 8 hr.
이론/모형
The ability of leaf extract to prevent Fe2+/H2O2 induced decomposition of deoxyribose was assessed using the method of Halliwell and Gutteeridge (22).
Total phenolic concentrations were expressed as mg of tannic acid equivalent (TAE) per g of extract. Total flavonoid content was determined using a spectrometric method (19). Each extract (0.
성능/효과
In conclusion, we found that A. gigas leaves are rich in phenolics and possess antioxidant activity. This suggests that A.
The efficient concentration (EC50) was estimated from the percentage of DPPH scavenging activity plotted as function of the concentration of Angelicae gigas leaf extract, which was expressed in terms of the concentration (mg/mL) required for 50% reduction of DPPH.
후속연구
gigas leaves are a good source of natural antioxidants and can be used against diseases caused by oxidative stress. Additional studies are currently underway to assess the in vitro antioxidant activity and to identify the active compounds in this plant.
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