[논문]황기(黃芪)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향

모충정; 이세나; 김명규; 김명희; 김형준; 조학준; 임강현

doi:10.6116/kjh.2011.26.1.059

황기(黃芪)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향
Effects of Astragali Radix Extracts on the Elastase Activity and DPPH and NO Scavenging Activities 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.26 no.1, 2011년, pp.59 - 63

모충정 (세명대학교 한의과대학) , 이세나 (세명대학교 한의과대학) , 김명규 (세명대학교 한의과대학) , 김명희 (세명대학교 간호학과) , 김형준 (세명대학교 한의과대학) , 조학준 (세명대학교 한의과대학) , 임강현 (세명대학교 한의과대학)

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Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. The defects of elastic matrix aggravate hypertension which is associated with alteration in the great arteries, arteries, and arterioles. The elastase inhibitors were undergoing in clinical studies about emphysema and pulmonary hypertension. This study was designed to investigate the effect of Astragali Radix extracts (AR) on elastase activity and anti-oxidative effects. Methods : The elastase inhibitory activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) and NO free radical scavenging activities of AR were measured. Results : The elastase activity was significantly inhibited by AR. The significant DPPH and NO free radical scavenging activities were observed in AR as well. Conclusion : AR showed the anti-elastase effects and anti-oxidative activities in vitro. These results suggest that AR may be a possible drug for the treatment of pulmonary emphysema and pulmonary hypertension.

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데이터처리

Significances of changes were evaluated using the Students' t-test.

이론/모형

It has been reported that reactive free radicals was induced by elastase in neutrophil and that they play a role in inflammation. Assays of the free radical scavenging capacity were carried out by the DPPH method. The free radical scavenging capacity of sample was measured at each concentration (0, 4, 20, 100, and 500 mg/ml).
The elastase activity was evaluated by using a modification of a previously reported method of Kraunsoe et al¹¹⁾. In order to evaluate the inhibition of elastase activity, the amount of released p-nitroaniline, which was hydrolyzed from the substrate, N-succinyl-Ala-Ala-Ala-p-nitroanilide, by elastase, was read with a maximum absorbance at 410 nm¹²⁾.

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