Identification of the SNP (Single Nucleotide Polymorphism) for Fatty Acid Composition Associated with Beef Flavor-related FABP4 (Fatty Acid Binding Protein 4) in Korean Cattle원문보기
In this study, we investigated the relationship between unsaturated fatty acids influencing beef flavor and four types of SNPs (c.280A>G, c.388G>A, c.408G>C and c.456A>G) located at exon 2, 3 and 4 of the FABP4 gene, which is a fatty acid binding protein 4 in Korean cattle (n = 513). When analyzing ...
In this study, we investigated the relationship between unsaturated fatty acids influencing beef flavor and four types of SNPs (c.280A>G, c.388G>A, c.408G>C and c.456A>G) located at exon 2, 3 and 4 of the FABP4 gene, which is a fatty acid binding protein 4 in Korean cattle (n = 513). When analyzing the relationship between single genotype, fatty acids and carcass trait, individuals of GG, GG, CC and GG genotypes that are homozygotes, had a higher content of unsaturated fatty acids and marbling scores than other genotypes (p<0.05). Then, haplotype block showed strong significant relationships not only with unsaturated fatty acids (54.73%), but also with marbling scores (5.82) in $ht1{\times}ht1$ group (p<0.05). This $ht1{\times}ht1$ group showed significant differences with unsaturated fatty acids and marbling scores that affected beef flavor in Korean cattle. Therefore, it can be inferred that the $ht1{\times}ht1$ types might be valuable new markers for use in the improvement of Korean cattle.
In this study, we investigated the relationship between unsaturated fatty acids influencing beef flavor and four types of SNPs (c.280A>G, c.388G>A, c.408G>C and c.456A>G) located at exon 2, 3 and 4 of the FABP4 gene, which is a fatty acid binding protein 4 in Korean cattle (n = 513). When analyzing the relationship between single genotype, fatty acids and carcass trait, individuals of GG, GG, CC and GG genotypes that are homozygotes, had a higher content of unsaturated fatty acids and marbling scores than other genotypes (p<0.05). Then, haplotype block showed strong significant relationships not only with unsaturated fatty acids (54.73%), but also with marbling scores (5.82) in $ht1{\times}ht1$ group (p<0.05). This $ht1{\times}ht1$ group showed significant differences with unsaturated fatty acids and marbling scores that affected beef flavor in Korean cattle. Therefore, it can be inferred that the $ht1{\times}ht1$ types might be valuable new markers for use in the improvement of Korean cattle.
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문제 정의
FABP4, which is highly associated with oleic acid, is an important factor for beef flavor, and has been considered a valuable factor for use in improvement of beef quality. Therefore, this study was conducted to obtain genetic information on exonic SNPs of FABP4 and their association with unsaturated fatty acids which could make them important factors in higher beef quality and for application of these factors for improvement of beef flavor in Korean cattle.
제안 방법
In general, they were weaned at 6 months of age, castrated at 6 months of age, fed with growth stage feed for 18 months, and given a high concentration diet in the last 6 months. Twenty-four hours after slaughter, carcass weight was measured and the carcass traits were dissected at the last rib and the first lumber vertebra according to the Animal Product Grading System of Korea for measurement of carcass traits. All steers were slaughtered at 941 days of age (mean±SD:941.
대상 데이터
Steers in Gyeonbuk province were fed according to the feeding program of each farmer. They were the progeny of 18 sires. The pedigree records of 513 steers were collected by the Charmpoom Hanwoo Research Institution.
데이터처리
Associations between individual SNPs and carcass traits and fatty acid composition in the 513 animals were analyzed by mixed ANCOVA (analysis of covariance) linear model using SPSS v19.0 software (SPSS Inc., Chicago, IL, USA). The following model was used: Yijk = μ+Si+Pj+Gk+β×age+eijk in which Yijk is the phenotypic observations of traits, μ is the overall mean of each trait, Si is the random effect of sire, Pj is the fixed effect of the place at the calving (14 classes), Gk is fixed effect of the SNP genotypes, β is a regression coefficient, age is a covariate for age in days at slaughter, and eijk is a random residual.
Significant differences among mean values of different genotypes were calculated using Duncan’s multiple range test and determined by p<0.05.
이론/모형
, 2005). Hardy-Weinberg equilibrium (HWE) in the examined population was tested by comparing expected and observed genotype frequencies using the Chi-square test.
Total lipids were extracted from approximately 500 mg of m. longissimus dorsi muscle with chloroform: methanol (2:1, vol/vol) according to the methods of Folch (Folch et al., 1957). Extracted lipids were then methylated by the method of O’Keefe (O’Keefe et al.
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