고 콜레스테롤을 급여한 SD-Rat에서 효소처리한 토마토케이크가 고지혈증에 미치는 영향 Hypolipidemic Effects of Enzyme-Treated Tomato Cake on Sprague-Dawley Rats Fed a High Cholesterol Diet원문보기
SD-rat에 대조구(Control), 고 콜레스테롤(HC), 3.5% 펙틴-가수분해 효소(Viscozyme L)로 처리된 토마토 케이크(ETC)를 각각 4주 간 급이하였다. 첫 일주일에는 HC-급이군의 체증 증가가 151.7%로 유의적 증가를 보였지만(p<0.05), ETC-급이군의 경우는 115.4%를 보였다. Control- 및 ETC-급이군의 사료 효율은 각각 $0.236{\pm}0.041$ 및 $0.447{\pm}0.030$으로 HC-급이군의 경우($0.355{\pm}0.034$)에 비하여 유의적인 차이를 보였다(p<0.05). ETC-급이군의 신장 및 부고환의 지방체는 각각 0.303 및 0.274 g 씩 감소하였다. HC-와 ETC-급이군 간의 혈청 콜레스테롤, 트리글리세라이드, HDL- 및 LDL-콜레스테롤 수치의 유의적인 차이는 보이지 않았다. ETC-급이군의 경우 2주 급이시 ABTS radicals로 평가한 총 항산화 활성은 약 1.1배 높았으며, 2 및 4주간 급이시 glutathione peroxidase 활성은 각각 2.5 및 1.3배 높게 나타났다.
SD-rat에 대조구(Control), 고 콜레스테롤(HC), 3.5% 펙틴-가수분해 효소(Viscozyme L)로 처리된 토마토 케이크(ETC)를 각각 4주 간 급이하였다. 첫 일주일에는 HC-급이군의 체증 증가가 151.7%로 유의적 증가를 보였지만(p<0.05), ETC-급이군의 경우는 115.4%를 보였다. Control- 및 ETC-급이군의 사료 효율은 각각 $0.236{\pm}0.041$ 및 $0.447{\pm}0.030$으로 HC-급이군의 경우($0.355{\pm}0.034$)에 비하여 유의적인 차이를 보였다(p<0.05). ETC-급이군의 신장 및 부고환의 지방체는 각각 0.303 및 0.274 g 씩 감소하였다. HC-와 ETC-급이군 간의 혈청 콜레스테롤, 트리글리세라이드, HDL- 및 LDL-콜레스테롤 수치의 유의적인 차이는 보이지 않았다. ETC-급이군의 경우 2주 급이시 ABTS radicals로 평가한 총 항산화 활성은 약 1.1배 높았으며, 2 및 4주간 급이시 glutathione peroxidase 활성은 각각 2.5 및 1.3배 높게 나타났다.
Sprague-Dawley rats were fed with normal (Control), high cholesterol (HC), and high cholesterol containing 3.5% pectin-hydrolytic activity industrial enzyme (Viscozyme L)-treated tomato cakes (ETC) for 4 weeks. In the first week, the weight gain of the HC-fed group was significantly increased to 151...
Sprague-Dawley rats were fed with normal (Control), high cholesterol (HC), and high cholesterol containing 3.5% pectin-hydrolytic activity industrial enzyme (Viscozyme L)-treated tomato cakes (ETC) for 4 weeks. In the first week, the weight gain of the HC-fed group was significantly increased to 151.7% (p<0.05), compared to the 115.4% weight gain of the ETC-fed group. The feed efficiencies of the Control- and ETC-fed groups ($0.236{\pm}0.041$ and $0.447{\pm}0.030$, respectively) were significantly different to those of HC-fed group ($0.355{\pm}0.034$) (p<0.05). The kidney and epididymal fat pad of the rats fed with ETC were decreased to 0.303 g and 0.274 g, respectively. There were no significant differences in serum cholesterol, triglycerides, high density lipoprotein-cholesterol or low density lipoprotein-cholesterol levels between the HC- and ETC-fed groups. The ETC-fed group exhibited approximately a 1.1-fold higher total antioxidant activity determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals than the HC-fed group at 2 weeks. Glutathione peroxidase activity of the ETC-fed group exhibited 2.5-and 1.3-fold increases at 2 and 4 weeks, respectively.
Sprague-Dawley rats were fed with normal (Control), high cholesterol (HC), and high cholesterol containing 3.5% pectin-hydrolytic activity industrial enzyme (Viscozyme L)-treated tomato cakes (ETC) for 4 weeks. In the first week, the weight gain of the HC-fed group was significantly increased to 151.7% (p<0.05), compared to the 115.4% weight gain of the ETC-fed group. The feed efficiencies of the Control- and ETC-fed groups ($0.236{\pm}0.041$ and $0.447{\pm}0.030$, respectively) were significantly different to those of HC-fed group ($0.355{\pm}0.034$) (p<0.05). The kidney and epididymal fat pad of the rats fed with ETC were decreased to 0.303 g and 0.274 g, respectively. There were no significant differences in serum cholesterol, triglycerides, high density lipoprotein-cholesterol or low density lipoprotein-cholesterol levels between the HC- and ETC-fed groups. The ETC-fed group exhibited approximately a 1.1-fold higher total antioxidant activity determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals than the HC-fed group at 2 weeks. Glutathione peroxidase activity of the ETC-fed group exhibited 2.5-and 1.3-fold increases at 2 and 4 weeks, respectively.
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문제 정의
The nutritional composition and biological evaluation of tomato seed cakes reported that the nutritional values of tomato seed cakes would improve the cereal-based farm animal feed quality (Rao 1991). The complexity of nutrients in tomato cakes might decrease the absorption of tomato cakes in animal feed, therefore tomato cakes were treated with a hydrolytic enzymes to increase the bioavailability of tomato cakes in this research. The overall goal of this research was to evaluate hypolipidemic activity of enzyme-treated tomato cakes (ETC) in high cholesterol fed rats, and the specific objective of this research was to evaluate the effect of ETC on weight gain, serum cholesterol levels, adipose tissue weight, and hepatic peroxides and enzymes of SD-rats fed with a high cholesterol diet for 4 weeks.
제안 방법
The complexity of nutrients in tomato cakes might decrease the absorption of tomato cakes in animal feed, therefore tomato cakes were treated with a hydrolytic enzymes to increase the bioavailability of tomato cakes in this research. The overall goal of this research was to evaluate hypolipidemic activity of enzyme-treated tomato cakes (ETC) in high cholesterol fed rats, and the specific objective of this research was to evaluate the effect of ETC on weight gain, serum cholesterol levels, adipose tissue weight, and hepatic peroxides and enzymes of SD-rats fed with a high cholesterol diet for 4 weeks.
대상 데이터
Three-week-old male Sprague-Dawley (SD) rats (137±1g) were purchased from Orientbio Company (Gyeonggi, South Korea) and initially maintained under a controlled light/dark cycle and temperature (22±2℃) for 7 days.
데이터처리
* Indicates significantly different in the mean± SD (n=5) analyzed by Duncan’s multiple range test (*p<0.05, **p<0.01, ***p<0.005).
* Indicates significantly different in the mean±SD (n=5) analyzed by Duncan’s multiple range test (*p<0.05, **p<0.01, ***p<0.005).
1) HC diet; Diet containing high cholesterol,2) ETC diet; Diet containing high cholesterol and 3.5% enzyme- treated tomato cake, Different superscripts within the column indicate significantly different in the mean±SD (n=5) analyzed by Duncan’s multiple range test (*p<0.05, **p<0.01, ***p<0.005).
1) HC diet; Diet containing high cholesterol,2) ETC diet; Diet containing high cholesterol and 3.5% enzyme-treated tomato cake, Different superscripts within the column indicate significantly different in the mean±SD (n=5) analyzed by Duncan’s multiple range test (*p<0.05, **p<0.01, ***p<0.005).
1) HC diet; Diet containing high cholesterol,2) ETC diet; Diet containing high cholesterol and 3.5% enzyme-treated tomato cake, Different superscripts within the row indicate significantly different in the mean±SD (n=5) analyzed by Duncan’s multiple range test (*p<0.05, **p<0.01, ***p<0.005).
Each experiment was performed three times in duplicates. Data were analyzed using the paired t-test in SAS software program (Statistical Analysis System Institute Inc., Cary, N.C., USA). Multiple mean comparisons among the samples were carried out by Duncan’s multiple range tests at P<0.
이론/모형
Proximate analysis of enzyme-treated tomato cakes was conducted following the standard AOAC method (AOAC 1997). The extraction and analysis of lycopene from the tomato was conducted following general procedures described by Kozukue & Friedman (2003).
The detailed formula of each diet is presented in Table 1. The compositions of experimental diets were based on the AIN-76 diet. The HC diet was prepared by supplementing 1.
성능/효과
However, there was no significant difference among the groups as the experiment proceeded to 4 weeks. Although the antioxidant activity determined by TBARS did not exhibit any significant difference among the groups both in 2 and 4 weeks (Fig. 2), the MDA value of the ETC-fed group apparently reduced approximately 85%, compared to that of the HC-fed group in 4 weeks (Fig. 1B). Cho et al.
4% of the HC-fed group, respectively. The results indicated that the addition of ETC to the feed was able to reduce the weight gains of SD-rats fed with high cholesterol in 1 week. However, the body weight gains of the entire groups were not significantly different after 1 week.
05); however, there was no significant difference between the two groups after one week. Therefore, feeding SD-rats with ETC could reduce the weight gains in the early time period, but the weight reduction effects were not continued after the 1 week-feeding period, and ETC did not affect the feeding efficiencies of SD-rats fed with high cholesterol.
참고문헌 (20)
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