[논문]어류 병원성 균주 Edwardsiella tarda에 대한 키토산-실버 나노입자의 항박테리아 효과

이영득; 조종기; 이제희

doi:10.17555/ksvc.2014.12.31.6.502

[국내논문] 어류 병원성 균주 Edwardsiella tarda에 대한 키토산-실버 나노입자의 항박테리아 효과
Chitosan Silver Nano Composites (CAgNCs) as Antibacterial Agent Against Fish Pathogenic Edwardsiella tarda 원문보기

Journal of veterinary clinics = 한국임상수의학회지, v.31 no.6, 2014년, pp.502 - 506

(충남대학교 수의과대학 &기후변화질병 제어 수의인력양성사업팀) , (제주대학교 해양과학대학 해양의생명과학부) , 이영득 (한국해양과학기술원) , 조종기 (충남대학교 수의과대학 &기후변화질병 제어 수의인력양성사업팀) , 이제희 (제주대학교 해양과학대학 해양의생명과학부)

초록
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최근들어 나노입자들을 활용한 다양한 연구들을 통해 생리활성 능력이 입증되고 있다. 본 연구에서는 어류 병원성 균주인 Edwardsiella tarda에 대해 키토산-실버 나노입자(CAgNCs)의 항박테리아 활성을 측정하였다. CAgNCs의 E. tarda에 대한 최소성장억제농도 및 최소살균농도를 확인한 결과 각각 $25{\mu}g/mL$와 $125{\mu}g/mL$임을 확인할 수 있었으며, 주사전자현미경으로 관찰결과 CAgNCs 처리구가 대조구에 비해 E. tarda의 세포벽 손상을 강하게 일으킨 것을 확인할 수 있었다. 더 나아가, CAgNCs는 E. tarda의 세포내 활성산소를 농도와 시간 의존적으로 증가시킴을 확인하였고, 이는 CAgNCs가 E. trada의 산화스트레스를 발생시켜 세포의 사멸을 유발시킨 것으로 예측된다. 또한, MTT assay 결과 CAgNCs를 E. tarda에 $100{\mu}g/mL$의 농도로 처리했을 때 최저 세포 생존능을 나타내었다. 이러한 결과들은 CAgNCs가 병원성 미생물 조절을 위한 항미생물제로서의 응용 가능성이 크다는 것을 보여주고 있다.

Abstract ▼ AI-Helper

Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterial properties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were $25{\mu}g/mL$ and $125{\mu}g/mL$, respectively. The field emission scanning electron microscope (FE-SEM) image of CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore, treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentration and time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition, MTT assay results showed that the lowest cell viability at $100{\mu}g/mL$ of CAgNCs treated E. tarda. Overall results of this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.

주제어

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문제 정의

This study demonstrates the potential application of CAgNCs to control E. tarda. Previous study showed that MIC and MBC of AgNPc as 124 ± 9.

제안 방법

Cell viability was determined by MTT assay after treatment with different concentrations of CAgNCs (6.25, 12.5, 25, 50, 75 and 100µg/mL).

데이터처리

All data were illustrated as means ± SD for triplicate reactions. Statistical analysis was performed using unpaired, twotailed t-test to calculate the P-value using GraphPad program (GraphPad Software, Inc.). The significant difference was defined at P < 0.

성능/효과

tarda cells upon CAgNCs treatment may cause destruction of cell structures. In conclusion, this study showed that CAgNCs have potent antibacterial activities against E. tarda. The growth and reproduction of E.
Result showed that gradual increase of the diameter of inhibitory zone with respect to CAgNCs concentrations (12.5-75 µg/disc) in a dose dependent manner (Fig 1).
5, 25, 50, and 75 µg/mL. The cell viability (by MTT assay) results revealed that the viable E. tarda cell counts were significantly decreased by CAgNCs in a dose dependent manner (Fig 5). The 100 µg/mL CAgNCs was shown substantial growth reduction of E.

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