[논문]한국 내 물쇠뜨기 6개 집단의 RAPD 변이와 표현형 관계

허만규; 최재원; 이장섭; 진보규; 김혜경

doi:10.5352/jls.2014.24.6.612

한국 내 물쇠뜨기 6개 집단의 RAPD 변이와 표현형 관계
RAPD Variation and Phenetic Relationships for Six Populations of Equisetum pratense in Korea 원문보기

생명과학회지 = Journal of life science, v.24 no.6 = no.170, 2014년, pp.612 - 617

허만규 (동의대학교 분자생물학과) , 최재원 (부산일과학고등학교) , 이장섭 (부산일과학고등학교) , 진보규 (부산일과학고등학교) , 김혜경 (부산일과학고등학교)

초록
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한국 내 물쇠뜨기 여섯 개 자연 집단의 표현형적 유연 관계를 RAPD 마커에 근거하여 집단수준에서 조사하였다. 또한 RAPD로 물쇠뜨기 집단의 유전적 다양성과 집단구조를 분석하였다. 물쇠뜨기는 평균 26.7%의 유전적 다형성을 나타내었다. 물쇠뜨기는 대립유전자좌위당 적은 수의 좌위(1.267)와 유효한 유전자좌위(1.176)를 나타내었다. 물쇠뜨기의 유전적 다양도(H)는 0.102로 유사한 생활사를 가진 다른 종에 비해 낮았다. 전체 유전적 다양도($H_T$)는 0.112(OPD-07)에서 0.445(OPD-16)까지였으며 평균은 0.141이었다. 전체 유전적 다양도에서 집단 내 다양도($H_S$)는 0.102로 낮았다. 이런 낮은 물쇠뜨기의 다양도는 무성적 생식, 작은 집단 크기, 집단화 과정 등으로 설명될 수 있었다. 유전자좌위에 근거한 집단간 분화를 나타내는 다양도는 비율은 OPD-07의 0.129에서 OPD-09에 0.455로 평균은 0.277이었다. 전체 유전적 변이에서 집단간 변이는 27.7%였으며 나머지 변이의 72.3%는 집단 내에 있었다. 본 결과는 물쇠뜨기의 분류학적 연구 및 집단유전학에 기여할 수 있을 것이다.

Abstract ▼ AI-Helper

The phenetic relationships among six natural populations of Equisetum pratense in Korea were investigated at the population level by constructing a tree based on Random Amplified Polymorphic DNA (RAPD) markers. RAPD analysis was also conducted to estimate genetic diversity and the population structure of E. pratense. A mean of 26.7% at the six population levels indicated polymorphism. E. pratense was found to have fewer alleles per locus (1.267) and fewer effective alleles per locus (1.176). Genetic diversity (0.102) in E. pratense is lower than the average for species with similar life history traits. Total genetic diversity values (HT) varied between 0.112 (OPD-07) and 0.445 (OPD-16), for an average overall polymorphic locus of 0.141. Inter-locus variation in the within-population genetic diversity ($H_S$) was low (0.102). Asexual reproduction, small population size, and the colonization process are proposed as possible factors contributing to the observed low genetic diversity in E. pratense. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.129 for OPD-07 to 0.455 for OPD-09, with a mean of 0.277. This indicated that about 27.7% of the total variation was among populations. Thus, genetic variation (72.3%) resided within populations. This study contributes new information for research on the taxonomy and population genetics of E. pratense.

주제어

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문제 정의

The objective of study is to estimate the level of genetic diversity in the species. We wanted to detect the pattern of differentiation and speciation using RAPD makers

대상 데이터

Perhaps the main reason for the success of RAPD analysis is the gain of a large number of genetic markers that require small amounts of DNA without the requirement for cloning, sequencing or any other form of the molecular characterization of the genome of the species in question [1]. In this study six populations of E. pratense from Korea were analyzed for RAPD markers. We expected that the RAPD analyses assess the amount and structure of genetic diversity within and among populations and we successfully assess the genetic relationships among the local populations of E.
, Grand Island, New York, USA) according to the manufacturer’s protocol. Twenty arbitrarily chosen 10-mer primers, the kit A (OPA-01 to 20) and kit D (OPD-01 to 20) of Operon Technologies (Alameda, Co., USA) were used. Amplification reactions were performed in 0.

이론/모형

A phenetic tree was constructed by the neighbor joining (NJ) method [20] using the NEIGHBOR program in PHYLIP version 3.57 [4].
DNA was extracted using the plant DNA Zol Reagent (Life Technologies Inc., Grand Island, New York, USA) according to the manufacturer’s protocol.

성능/효과

In a simple measure of inter-populations variability i.e. the percentage of polymorphic bands, the Yecheon population exhibited the highest variation (35.3%) among populations and the Busan population the lowest (20.6%) (Table 3). The average number of alleles per locus (A) was 1.
Total genetic diversity values (H_T) varied between 0.112 (OPD-07) and 0.445 (OPD-16), for an average over all polymorphic loci of 0.141 (Table 4). Interlocus variation in the within-population genetic diversity (H_S) was low (0.

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