[논문]Gallic acid의 Coxsackievirus B3와 Coxsackievirus B4에 대한 항바이러스 효과

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doi:10.4014/kjmb.1404.04004

Gallic acid의 Coxsackievirus B3와 Coxsackievirus B4에 대한 항바이러스 효과
Antiviral Activity of Gallic Acid against Coxsackievirus B3 and Coxsackievirus B4 원문보기

한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, v.42 no.2, 2014년, pp.207 - 210

초록
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바이러스 감염은 감염된 숙주세포에서 활성산소를 유도할 수 있으며, 항산화제들은 많은 바이러스에 대해서 항바이러스 능을 갖는다고 보고되었다. 본 연구에서 cytopathic effect (CPE) reduction 방법에 의한 항바이러스 활성 평가에서 gallic acid는 현저하게 CPE형성을 감소시키면서 coxsackievirus B3 (CB3)와 coxsackievirus B4 (CB4)에 대해 항바이러스 활성을 나타내었다. 그러나 rabavirin은 CB3와 CB4에 대해 CPE를 막지 못하면서 약한 항바이러스 활성을 나타내었다. 그럼으로 gallic acid의 CB3와 CB4에 대한 항바이러스 활성은 항산화제 효능 때문인 것으로 사료된다.

Abstract ▼ AI-Helper

Viral infections are capable of inducing reactive oxygen species (ROS) production in the infected cells and antioxidants have been reported to have antiviral activities against many viruses. In this study, an antiviral assay using the cytopathic effect (CPE) reduction method revealed that gallic acid possesses good anti-coxsackievirus B3 (CB3) and coxsackievirus B4 (CB4) activities, reducing the formation of visible CPE. However, ribavirin did exhibit weak anti-CB3 and CB4 activities and was unable to prevent CPE. Therefore, we conclude that the inhibition of CB3 or CB4 production by gallic acid may be due to its general action as an antioxidant.

주제어

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제안 방법

Hence, this present study was undertaken to evaluate the antiviral activity against CB3 and CB4, and the effect of gallic acid on CB3 or CB4-induced CPE. Furthermore, Our result suggest that the antiviral activity of gallic acid against CB3 and CB4 is correlated with the antioxidant activity of gallic acid.
Results are presented as the mean percentage values obtained from three independent experiments carried out in triplicate ± S.D. (A) Antiviral activity of gallic acid against CB3, (B) antiviral activity of gallic acid against CB4, (C) cytotoxicity of gallic acid in Vero Cells.

대상 데이터

In our experiments, sulforhodamine B (SRB) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were a reagent grade.
This research was supported by Kwangju Women's University in 2014, Korea.

이론/모형

Assays of antiviral activity and cytotoxicity were evaluated by the previous reported SRB method using cytopathic effect (CPE) reduction [3]. Infectivity of virus stock was determined by the SRB method and was determined as infectivity of the virus by SRB ID₅₀ (50% infective dose). The results were transformed to percentage of controls and were drawn the dose-response curves.

참고문헌 (8)

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상세보기
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상세보기
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상세보기
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상세보기
Weiss L, Hildt E, Hofschneider PH. 1996. Anti-hepatitis B virus activity of N-acetyl-L-cysteine (NAC): new aspects of a wellestablished drug. Antiviral Res. 32: 43-53.

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