The Anti-Adipogenic Activity of a New Cultivar, Pleurotus eryngii var. ferulae 'Beesan No. 2', through Down-Regulation of PPAR γ and C/EBP α in 3T3-L1 Cells원문보기
Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'B...
Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes. Pleurotus eryngii var. ferulae is one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects. Biological activities of 'Beesan No. 2', a new cultivar of Pleurotus eryngii var. ferulae, have not yet been reported. In this study, we found that CEBT suppressed adipogenesis in 3T3-L1 cells through inhibition of key adipogenic transcription factors, such as peroxisome proliferatoractivated receptor ${\gamma}$ and CCAAT/enhancer binding protein ${\alpha}$. Additionally, CEBT reduced the expression of the IRS/PI3K/Akt signaling pathway and its downstream factors, including mammalian target of rapamycin and p70S6 kinase, which stimulate adipogenesis. Furthermore, ${\beta}-catenin$, a suppressor of adipogenesis, was increased in CEBT-treated cells. These results indicate that Pleurotus eryngii var. ferulae 'Beesan No. 2' effectively inhibited adipogenesis, so this mushroom has potential as an anti-obesity food and drug.
Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes. Pleurotus eryngii var. ferulae is one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects. Biological activities of 'Beesan No. 2', a new cultivar of Pleurotus eryngii var. ferulae, have not yet been reported. In this study, we found that CEBT suppressed adipogenesis in 3T3-L1 cells through inhibition of key adipogenic transcription factors, such as peroxisome proliferatoractivated receptor ${\gamma}$ and CCAAT/enhancer binding protein ${\alpha}$. Additionally, CEBT reduced the expression of the IRS/PI3K/Akt signaling pathway and its downstream factors, including mammalian target of rapamycin and p70S6 kinase, which stimulate adipogenesis. Furthermore, ${\beta}-catenin$, a suppressor of adipogenesis, was increased in CEBT-treated cells. These results indicate that Pleurotus eryngii var. ferulae 'Beesan No. 2' effectively inhibited adipogenesis, so this mushroom has potential as an anti-obesity food and drug.
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가설 설정
Nowadays, investigation of the beneficial effects of several natural compounds in the adipocyte-related bioprocess is a new strategy of anti-obesity research [24]. In this study, we investigated how CEBT down-regulated adipogenic differentiation in 3T3-L1 mouse preadipocytes. The results suggested that CEBT inhibited the IRS/PI3K/ Akt pathway and subsequently decreased the expression of key adipogenic transcription factors, such as C/EBP α and PPAR γ.
데이터처리
Differences between means of each experimental group were analyzed using one-way analysis of variance followed by Dunnett’s multiple-comparison test with Prism 5.0 software (Graph-Pad Software, USA).
성능/효과
According to the results, C/EBP α and PPAR γ protein expression levels were decreased by CEBT, similar to their mRNA expression.
Then, palmitate serves as a substrate for long-chain fatty acid [4]. As shown in the Oil Red O staining and triglyceride assay results (Fig. 2), CEBT decreased the lipid accumulation on the treated adipocytes compared with mature adipocytes. Besides that, the protein levels of SREBP1 and FAS were reduced by CEBT (Fig.
In conclusion, we found that CEBT inhibited adipogenesis in 3T3-L1 preadipocytes through inhibition of key adipogenic factors, including PPAR γ, C/EBP α, and IRS/PI3K/Akt signaling.
In our results, CEBT increased the expression of phospho-GSK3β (Ser 9) without any alteration of total GSK3β expression.
In our results, CEBT significantly down-regulated the mRNA and protein expression levels of PPAR γ as well as C/EBP α in a dosedependent manner.
The results suggested that CEBT inhibited the IRS/PI3K/ Akt pathway and subsequently decreased the expression of key adipogenic transcription factors, such as C/EBP α and PPAR γ.
2B and 2C), retained Oil Red O dye and intracellular triglyceride values were decreased in a dose-dependent manner when cells were treated with CEBT. These results demonstrated that CEBT reduced the accumulation of lipid in 3T3-L1 during adipogenic differentiation without cell cytotoxicity.
These results indicated that CEBT inhibited the expression of pivotal adipogenic transcription factors, C/ EBP α and PPAR γ, at the levels of mRNA and protein.
4B, CEBT down-regulated the activation of mTOR and P70S6K, but did not have any effect on the total expression of mTOR as well as P70S6K. These results indicated that the activation of mTOR and P70S6K was reduced by CEBT, because CEBT inhibited their upstream pathway, such as IRS/PI3K/Akt signaling. This inhibitory ability of CEBT can reduce lipogenesis during adipogenic differentiation of 3T3-L1 preadipocytes.
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