[논문]Fructus Amomi Cardamomi Extract Inhibits Coxsackievirus-B3 Induced Myocarditis in a Murine Myocarditis Model

Lee, Yun-Gyeong; Park, Jung-Ho; Jeon, Eun-Seok; Kim, Jin-Hee; Lim, Byung-Kwan

doi:10.4014/jmb.1605.05056

Fructus Amomi Cardamomi Extract Inhibits Coxsackievirus-B3 Induced Myocarditis in a Murine Myocarditis Model 원문보기

Journal of microbiology and biotechnology, v.26 no.11, 2016년, pp.2012 - 2018

Lee, Yun-Gyeong (Department of Biomedical Science, Jungwon University) , Park, Jung-Ho (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) , Jeon, Eun-Seok (Cardiac and Vascular Center, Samsung Medical Center, Sungkyunkwan University School of Medicine) , Kim, Jin-Hee (College of Herbal Bio-Industry, Daegu Haany University) , Lim, Byung-Kwan (Department of Biomedical Science, Jungwon University)

Abstract ▼ AI-Helper

Coxsackievirus B3 (CVB3) is the main cause of acute myocarditis and dilated cardiomyopathy. Plant extracts are considered as useful materials to develop new antiviral drugs. We had previously selected candidate plant extracts, which showed anti-inflammatory effects. We examined the antiviral effects by using a HeLa cell survival assay. Among these extracts, we chose the Amomi Cardamomi (Amomi) extract, which showed strong antiviral effect and preserved cell survival in CVB3 infection. We investigated the mechanisms underlying the ability of Amomi extract to inhibit CVB3 infection and replication. HeLa cells were infected by CVB3 with or without Amomi extract. Erk and Akt activities, and their correlation with virus replication were observed. Live virus titers in cell supernatants and viral positive- and negative-strand RNA amplification were measured. Amomi extract significantly increased HeLa cell survival in different concentrations ($100-10{\mu}g/ml$). CVB3 capsid protein VP1 expression (76%) and viral protease 2A-induced eIF4G1 cleavage (70%) were significantly decreased in Amomi extract ($100{\mu}g/ml$) treated cells. The levels of positive- (20%) and negative-strand (80%) RNA were dramatically decreased compared with the control, as revealed by reverse transcription-PCR. In addition, Amomi extract improved mice survival (51% vs 26%) and dramatically reduced heart inflammation in a CVB3-induced myocarditis mouse model. These results suggested that Amomi extract significantly inhibited Enterovirus replication and myocarditis damage. Amomi may be developed as a therapeutic drug for Enterovirus.

주제어

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제안 방법

In this study, we screened a number of plant extracts and selected Amomi extract as a candidate for a strong anti-CVB3 agent. This extract inhibited CVB3 replication, having a strong antiviral effect in HeLa cells.

대상 데이터

Five-week-old male Balb/c mice were infected by 2 × 103 plaque-forming units (PFUs) of CVB3 by intraperitoneal injection.

데이터처리

The data are presented as the mean ± SEM. All measured data were examined using the Student t-test by Prism 4.0 (GraphPad Software, USA). Mice survival was represented by the Kaplan–Meier survival curve and analysis method.

이론/모형

Mice survival was represented by the Kaplan–Meier survival curve and analysis method.

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