[논문]Variation in Phenotypic Characteristics and Contents of Sesquiterpene Lactones in Lettuce (Lactuca sativa L.) Germplasm

Sung, Jung-Sook; Hur, On-Sook; Ryu, Kyoung-Yul; Baek, Hyung-Jin; Choi, Susanna; Kim, Sang-Gyu; Luitel, Binod Prasad; Ko, Ho-Cheol; Gwak, Jae-Gyun; Rhee, Ju-Hee

doi:10.7732/kjpr.2016.29.6.679

Variation in Phenotypic Characteristics and Contents of Sesquiterpene Lactones in Lettuce (Lactuca sativa L.) Germplasm 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.29 no.6, 2016년, pp.679 - 689

Sung, Jung-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Hur, On-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Ryu, Kyoung-Yul (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Baek, Hyung-Jin (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Choi, Susanna (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Kim, Sang-Gyu (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Luitel, Binod Prasad (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Ko, Ho-Cheol (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Gwak, Jae-Gyun (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) , Rhee, Ju-Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)

Abstract ▼ AI-Helper

Lettuce is an important annual leafy vegetable and bitterness is its potent flavor character. Lettuce germplasm differ their phenotypic characters and sesquiterpene lactones (SLs) contents which are important for consumer's acceptance. This study was carried out to evaluate the phenotypic characters and SLs contents in one hundred lettuce germplasm in Jeonju, Korea. Twenty-three agro-morphological (16 qualitative and 7 quantitative) traits and two SLs (lactucin and lactucopicrin) contents were studied in these germplasm. Germplasm exhibited the variation in qualitative and quantitative characters. Average plant weight was 423.9 g with a range from 116.0 to 905.0 g. Lactucin content was varied from 19.7 (IT 294226) to $194.4{\mu}g/g$ (IT 294298) with an average concentration of $84.7{\mu}g/g$. Lactucopicrin ranged from 82.5 (IT 300134) to $2228.6{\mu}g/g$ (IT 294210) with an average concentration of $586.3{\mu}g/g$. Total SLs content was ranged from 120.1 (IT 300134) to 2286.6 (IT 294210)${\mu}g/g$ with the average concentration of $671.0{\mu}g/g$. Significant ($p{\leq}0.05$) differences were found between crisp head and butter head germplasm for lactucin, lactucopicrin and total SLs content. Crisp head germplasm revealed the highest average lactucin ($112.9{\mu}g/g$), lactucopicrin ($734.8{\mu}g/g$) and total SLs content ($847.7{\mu}g/g$). Crisp head and leafy type germplasm exhibited more total SLs content (847.7 and $744.7{\mu}g/g$, respectively) than cos ($524.9{\mu}g/g$) and butter head type ($519.4{\mu}g/g$). Principal component analyses of the quantitative traits indicated that the first principal component axis accounted more than 91% of the total variation. This study revealed the ample genetic variation in the agro-morphological traits and SLs contents to support the selection for improved lettuce varieties.

주제어

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문제 정의

However, studies on morphological and biochemical (sesquiterpene lactones) characterization among the lettuce germplasm are not well documented yet in Korea. Therefore, this study was done to assess the variation in phenotypic characters and SLs content in lettuce germplasm collected in the genebank of Korea.

제안 방법

To summarize, the study showed variation among lettuce germplasm for all phenotypic and SLs contents. We employed sixteen qualitative traits and nine quantitative traits for the study of 100 lettuce germplasm.

대상 데이터

The methanol was evaporated under reduced pressure in a High Capacity Centrifugal Evaporator (Genevac, HT-4X, ͌5 ㎜ Hg, 30~35°C). It was analyzed using standard lactucin (C₁₅H₁₆O_5, 276.3) lactucopicrin (C₂₃H₂₂O₇, 410). The extract was then partitioned two times between water/chloroform (20 ㎖; 1:1 mixture by volume) with the chloroform separated, dried over anhydrous magnesium sulfate, and evaporated using a High Capacity Centrifugal Evaporator (Genevac, HT-4X, ͌5 ㎜ Hg, 30~35°C).
This experiment was conducted at the Research Farm of National Agrobiodiversity Center, Jeonju (35°49′18″ N 127°08′ 56″ E), Republic of Korea.

데이터처리

, Chicago, IL, USA). Lettuce accessions were further categorized based on plant growth habit i.e. leafy type, crisp head, cos (romaine), butter head, stem and oil seed and, agronomic and SLs contents data were further analyzed using one-way analysis of variance followed by Student-Newman-Keuls test for multiple comparisons of means. Principal component analysis (PCA) was performed on qualitative and quantitative characters using Microsoft Excel (version 10.
leafy type, crisp head, cos (romaine), butter head, stem and oil seed and, agronomic and SLs contents data were further analyzed using one-way analysis of variance followed by Student-Newman-Keuls test for multiple comparisons of means. Principal component analysis (PCA) was performed on qualitative and quantitative characters using Microsoft Excel (version 10.0, Microsoft, Redmond, WA, USA), Multibase program (http://www.numerical dynamics.com).

성능/효과

PCA of the 16 qualitative traits revealed that the first six axes (PC1-PC6) cumulatively accounted for 85.8% of the total variation observed among the germplasm (Table 6). The first PC axis accounted 41.
PCA using 10 quantitative traits (Table 7) indicated that the first two principal components (PC1 and PC2) contributed 99.1% of the total variation observed among the germplasm.

참고문헌 (25)

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