[논문]오이 떡잎에서 노쇠화 관련 유전자들의 식물 호르몬에 대한 반응 연구

차현정; 김대재

doi:10.5352/jls.2016.26.8.895

[국내논문] 오이 떡잎에서 노쇠화 관련 유전자들의 식물 호르몬에 대한 반응 연구
Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development 원문보기

생명과학회지 = Journal of life science, v.26 no.8 = no.196, 2016년, pp.895 - 903

차현정 (충북대학교 대학원 과학교육과) , 김대재 (충북대학교 사범대학 생물교육과)

초록
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본 연구는 발달중인 오이 떡잎에 식물호르몬을 처리하여 나타나는 노쇠화 관련 유전자들(SAG)의 유전자 발현 반응을 탐구하기 위하여 수행되었다. 따라서 선별된 오이의 노쇠화 관련 유전자들에 대하여 그들의 유전자 발현 반응을 특정하기 위하여 역전사-중합효소연쇄반응(RT-PCR)을 통하여 조사되었다. 파종 후 14일 된 오이의 떡잎을 절취한 후 100 μM IAA또는 zeatin 용액 위에 두고 빛이 있거나 없는 조건에서 4일 째 까지 처리하였다. 떡잎은 2일 간격으로 회수하여 총RNA추출과 RT-PCR의 시료로 사용하였다. RT-PCR 결과에 따르면 몇몇 오이의 SAG 전사체들은 처리기간 동안에 상당한 변화를 나타냈다. 에틸렌 반응 실험에서는 처리 1일 후 반응을 나타낸 PCK, SAG 158과 SAG 288을 제외한 대부분의 오이 SAGs는 즉각 반응을 보이지 않았으나 ICL과 SAG 281은 10일 처리 후 노란 떡잎에서 강한 반응을 나타냈다. 이러한 결과들은 몇몇 오이의 SAGs가 외부적 자극에 대하여 영양 결핍이나 노쇠화 반응을 나타냄을 의미한다. 떡잎의 발달 동안에 이와 같은 노쇠화 유도 반응은 기관의 노쇠화에서 SAGs의 대사적 역할과 기능을 이해 할 수 있는 정보를 제공한다.

Abstract ▼ AI-Helper

This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence- associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

주제어

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문제 정의

The plant hormones such as auxin and cytokinin, regulate many aspects of plant growth and development [28, 33]. Furthermore, gas form ethylene, which organ senescence and fruit mature motivat- ing plant hormone [26, 31, 34] is another factor to SAGs study in this research to find out the genes reaction.
developing cucumber cotyledons. In this report, we discuss the selected cucumber SAGs biological function during cotyledon senescence and the reactions against the plant hormones in induced cotyledon senescence.
This is a probe study to find out the relations between stress and senescence correlation in cucumber cotyledon development. Application of plant hormone is considered as an internal cause of organ senescence.

가설 설정

**Amino acids numbers are matched length compared (L/C).

대상 데이터

Polymerase chain reaction (PCR) was performed for 30 cy- cles using MyCycler (BioRad, USA) with an AccuPower PCR PreMix Kit (Bioneer, Korea) by gene-specific primers (5'-3'), as described in a previous report [19]. All primers were manufactured through Bioneer Co (Daejeon, Korea). An equal volume (10 μl) of RT-PCR products were fractio- nated on 1.

성능/효과

In conclusion, we were able to confirm hormone-depend- ent cucumber SAGs such as ICL, PCK, SAG 66, SAG158, SAG281, SAG288, SAG 338, SAG3-5 and SAG11-6, using three different type of plant hormones. They can be catego- rized into two group of SAGs from this result as destruc- tion SAG and remobilization SAG; the first group would be SAG 66, 158, 281, 338 and 11-6 and, the second group would be ICL, PCK and SAG3-5.

후속연구

Therefore, these stimuli encouraged senescence development and re- lated genes reactions. Gene’s reaction was diverse to hor- mones, light and darkness that is interesting to preview the succeeding step of study for finding of specific signal route in cotyledon senescence. Oil seed germination associated ICL and PCK showed fairly similar reaction to hormones that implies corresponding signal routes as suggested in earlier observation [18].

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