[논문]Burkholderia tropica as a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions

Zarate, Ana; Florez, July; Angulo, Edgardo; Varela-Prieto, Lourdes; Infante, Cherlys; Barrios, Fredy; Barraza, Beatriz; Gallardo, D.I; Valdes, Jorge

doi:10.4014/jmb.1611.11059

Burkholderia tropica as a Potential Microalgal Growth-Promoting Bacterium in the Biosorption of Mercury from Aqueous Solutions 원문보기

Journal of microbiology and biotechnology, v.27 no.6, 2017년, pp.1138 - 1149

Zarate, Ana (Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta) , Florez, July (Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico) , Angulo, Edgardo (Microalgae Biotechnology Research Group, Faculty of Basic Sciences, University of Atlantico) , Varela-Prieto, Lourdes (Immunology and Molecular Biology Research Group, University of Atlantico) , Infante, Cherlys (Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena) , Barrios, Fredy (Faculty of Pharmaceutical Sciences, Campus of Zaragocilla, University of Cartagena) , Barraza, Beatriz (Faculty of Health Sciences, CIUL, Universuty Libre) , Gallardo, D.I (Department of Mathematics, Faculty of Engineering, University of Atacama) , Valdes, Jorge (Doctoral Program in Applied Sciences - Coastal Marine Systems, Faculty of Marine Sciences and Biological Resources, University of Antofagasta)

Abstract ▼ AI-Helper

The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the potential of Burkholderia tropica co-immobilized with Chlorella sp. in polyurethane discs for the biosorption of Hg(II) from aqueous solutions and to evaluate the influence of different Hg(II) concentrations (0.041, 1.0, and 10 mg/l) and their exposure to different contact times corresponding to intervals of 1, 2, 4, 8, 16, and 32 h. As expected, microalgal bacterial biomass adhered and grew to form a biofilm on the support. The biosorption data followed pseudo-second-order kinetics, and the adsorption equilibrium was well described by either Langmuir or Freundlich adsorption isotherm, reaching equilibrium from 1 h. In both bacterial and microalgal immobilization systems in the co-immobilization of Chlorella sp. and B. tropica to different concentrations of Hg(II), the kinetics of biosorption of Hg(II) was significantly higher before 60 min of contact time. The highest percentage of biosorption of Hg(II) achieved in the co-immobilization system was 95% at pH 6.4, at 3.6 g of biosorbent, $30{\pm}1^{\circ}C$, and a mercury concentration of 1 mg/l before 60 min of contact time. This study showed that co-immobilization with B. tropica has synergistic effects on biosorption of Hg(II) ions and merits consideration in the design of future strategies for the removal of toxic metals.

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가설 설정

tropica in polyurethane discs. (B) Both microorganisms are randomly immobilized in the polymeric foam. (C) After co-cultivating for a short period of time, the two microorganisms share the cavities within the porous foams.

제안 방법

This study is experimental and was divided into two phases: a field phase and a laboratory phase.

대상 데이터

All experiments were carried out in triplicates (n = 3). To prove the normality and homogeneity of variances of the data, the nonparametric Kruskal-Wallis test (p ≤ 0.
041 mg/l Hg(II). All tests were carried out in triplicates.
The process described above was carried out in a total of 18 immobilization reactors.
Water samples were taken in a coastal wetland (Mallorquin swamp), located in the Colombian Caribbean coast in northeastern Colombia [37]. This is an aquatic ecosystem that is highly urbanized and industrialized, because the chemical, petrochemical, pharmaceutical, metal mechanical, agrochemical, and fishing industries are common activities in this area [38, 39].

데이터처리

Inferential analysis. Statistical analysis of variables influencing A- was conducted in the statistical program R ver. 3.3.1 by beta regression using the package Betareg [49, 50].

이론/모형

The results of the adsorption isotherm study are tabulated in Table 5. The experimental data were plotted according to both Langmuir and Freundlich isotherm equations (Eqs. (2) and (3)), and the goodness of fit was measured by the regression coefficient, R², from the adsorption isotherm curves.
To prove the normality and homogeneity of variances of the data, the nonparametric Kruskal-Wallis test (p ≤ 0.05) was used.

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