[논문]Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng

Hong, Jeongeui; Kim, Hogyum; Ryu, Hojin

doi:10.5010/jpb.2018.45.4.306

Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.45 no.4, 2018년, pp.306 - 314

Hong, Jeongeui (Department of Biology, Chungbuk National University) , Kim, Hogyum (Department of Biology, Chungbuk National University) , Ryu, Hojin (Department of Biology, Chungbuk National University)

Abstract ▼ AI-Helper

Korean ginseng (Panax ginseng) has long been cultivated as an important economic medicinal plant. Owing to the seasonal and long-term agricultural cultivation methods of Korean ginseng, they are always vulnerable to various environmental stress conditions. ABSCISIC ACID (ABA) is an essential plant hormone associated with seed development and diverse abiotic stress responses including drought, cold and salinity stress. By modulating ABA responses, plants can regulate their immune responses and growth patterns to increase their ability to tolerate stress. With recent advances in genome sequencing technology, we first reported the functional features of genes related to canonical ABA signaling pathway in P. ginseng genome. Based on the protein sequences and functional genomic analysis of Arabidopsis thaliana, the ABA related genes were successfully identified. Our functional genomic characterizations clearly showed that the ABA signaling related genes consisting the ABA receptor proteins (PgPYLs), kinase family (PgSnRKs) and transcription factors (PgABFs, PgABI3s and PgABI5s) were evolutionary conserved in the P. ginseng genome. We confirmed that overexpressing ABA related genes of P. ginseng completely restored the ABA responses and stress tolerance in ABA defective Arabidopsis mutants. Finally, tissue and age specific spatio-temporal expression patterns of the identified ABA-related genes in P. ginseng tissues were also classified using various available RNA sequencing data. This study provides ABA signal transduction related genes and their functional genomic information related to the growth and development of Korean ginseng. Additionally, the results of this study could be useful in the breeding or artificial selection of ginseng which is resistant to various stresses.

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제안 방법

To determine the functional roles of the identified genes in ABA signal transduction, the germination rate and resistance to drought stress were investigated with Col-0 and overexpressing lines in the rcar, snrk2.2/2.3 and snrk2.6 Arabidopsis genetic mutant backgrounds in the presence of ABA (Fig. 4). We initially confirmed the transgene expression level by a western blotting and germination rates of all wild type control and transgenic lines in the absence of ABA (Fig.
The genes predicted to play a role for ABA signaling in ginseng by blast method using the protein sequence information of genes involved in ABA signaling confirmed by functional genomic studies in Arabidopsis and several model systems. We identified total putative 67 protein sequences which could be ABA signaling components including 29 PYR1 / PYL / RCARs as ABA receptor proteins (PgPYL1 to PgPYL29), the negative regulatory 7 phosphatase (PP2C) ABI1 and 2 of ABA signaling (PgABI1a to PgABI1c, PgABI2a to PgABI2d), 14 phosphorylated enzyme SnRKs (PgSnRK1~ PgSnRK14), 5 transcription factor ABI3 (PgABI3a to PgABI3e), 4 ABI4 (PgABI4a to PgABI4d), 12 ABFs (PgABF1 to PgABF12) and 6 ABI5 (PgABI5a to PgABI5f), respectively (Table 1). The gene sequence information revealed that much number of ABA signal transduction related genes are presented in the P.

대상 데이터

, 2014). Electroporation was used to introduce the PgPYL3-pCAMBIA1303, PgPYL4-pCAMBIA1303 and PgSnRK1- pCB302ES, PgSnRK3-pCB302ES vector into Agrobacterium strain GV3101. All transgenes were integrated into the Arabidopsis genome by Agrobacterium-mediated floral dipping methods.

이론/모형

Electroporation was used to introduce the PgPYL3-pCAMBIA1303, PgPYL4-pCAMBIA1303 and PgSnRK1- pCB302ES, PgSnRK3-pCB302ES vector into Agrobacterium strain GV3101. All transgenes were integrated into the Arabidopsis genome by Agrobacterium-mediated floral dipping methods. Transgene expression was verified by immunoblotting.

성능/효과

ginseng genome compared to that of other plants. It is possible that this feature of P. ginseng is an evolutionary product to maintain the characteristics of heterozygous dielectrics and the ability of ginseng to cope with various external stresses with whole lifetime of more than 100 years. For the further functional genomic study of these predicted genes, the evolutionary similarity among the genes was confirmed based on the protein sequence information of ABA signal transduction genes of Arabidopsis (Fig.
We also found that the expression was regulated by exogenous ABA, suggesting that they are involved in ABA signal transduction. It was also confirmed that the ABA responsiveness was restored by complementation of the ginseng ABA related genes in ABA signaling defective Arabidopsis mutants.
3B). It was confirmed that ARR2-RFP and PgABF1-GFP were co-localized in the nucleus according to the coincidence of intracellular expression positions. These results suggest that ABA signal transduction genes identified in ginseng may play the same roles as previously reported in Arabidopsis research.
3A). When GFP expression was confirmed, all PYLs and SnRKs of P. ginseng were clearly detected in the nuclear and cytoplasm similar to Arabidopsis. The transcription factor, ABFs in Arabidopsis have been reported to be expressed in the nucleus since they are transcription factors (Yoshida et al.

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