선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- After incubation for 16 h at 37°C, the formation of plaque and its size were measured for further procedures.
- The specificity of KFS-YE was investigated by employing each overnight culture of bacteria (8 log CFU/ml), and a dot assay was performed following the procedures described for the methods of isolation, propagation, and purification of phages.
- To determine the temperature effect, 100 μl of KFS-YE (9 log PFU/ml) was mixed with 900 μl of SM buffer (pH 7) and incubated at various temperatures (4°C, 22°C, 37°C, 50°C, 60°C, 70°C, and 80°C) for 1 h. Finally, the plaque assay was performed for comparisons following the procedures described for the methods of isolation, propagation, and purification of phages. In addition, KFS-YE (9 log PFU/ml) in TA broth was stored at various temperatures (-80°C, -20°C, 4°C, and 22°C) to determine its stability during the storage period.
- To minimize damage from freezing, 20% glycerol was added to KFS-YE stored in TA broth at -80°C and -20°C. At 2-week intervals, the plaque assay was performed to measure the lytic activity of KFS-YE over the storage periods.
- The experiments were replicated three times and the experimental results are expressed as means ± standard deviations (SD).
- To elucidate the ability of KFS-YE to lyse Y. enterocolitica, the latent period and burst size were determined using a one-step growth curve analysis (Fig. 3). Because the definition of latent period is the time interval between the adsorption and the beginning of the first burst [27], it was determined to be 45 min.
- Based on the one-step growth curve, the multiplication parameters of KFS-YE, such as the latent period and burst size, were analyzed (Fig. 3). The length of the latent period depends on the type of phage, phage species, physiological conditions, type of host, and the composition of the medium and incubation temperature [33].
대상 데이터
- All bacterial strains with ATCC number and without ATCC number were provided from the American Type of Culture Collection and the laboratory in the Department of Plant and Food Sciences at Sangmyung University in Korea, respectively, except for Yersinia tuberculosis and Yersinia frederiksenii.
데이터처리
- Student’s paired t-test for two groups and a one-way analysis of variance (ANOVA) for more than two groups were used to compare the means using the GraphPad and InStatV.3 programs (GraphPad, USA).
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