[논문]상기생(Loranthus parasiticus) 추출물의 탄수화물 소화 효소 및 식후 고혈당 저해 효과

박민정; 박재은; 한지숙

doi:10.5352/jls.2020.30.1.18

상기생(Loranthus parasiticus) 추출물의 탄수화물 소화 효소 및 식후 고혈당 저해 효과
Inhibitory Effects of Loranthus Parasiticus Extract on Carbohydrate Digestive Enzymes and Postprandial Hyperglycemia 원문보기

생명과학회지 = Journal of life science, v.30 no.1, 2020년, pp.18 - 25

박민정 (동서대학교 식품영양학과) , 박재은 (부산대학교 식품영양학과) , 한지숙 (부산대학교 식품영양학과)

초록
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상기생(Loranthus parasiticus)은 뽕나무에 기생하는 겨우살이로 전세계에 널리 분포되어 있으며, 수 세기 동안 전통 의학의 성분으로 사용되어 왔고, 최근까지 항암, 항산화, 항비만, 항염증 효과 등이 연구되었으나, 탄수화물 효소나 식후 혈당 수치에 미치는 영향에 관한 연구는 부족한 실정이다. 본 연구는 Loranthus parasiticus 추출물 (LPE)이 탄수화물 분해 효소(α-glucosidase, α-amylase) 활성 억제와 streptozotocin (STZ)으로 유도된 당뇨병 마우스에서 식후 고혈당 완화 효과를 조사하였다. 그 결과 LPE는 농도에 비례하여 α-glucosidase와 α-amylase 활성을 억제 하였고, 각각의 IC₅₀ 값으로 0.121±0.007 및 0.157±0.004 mg/ml을 나타내어 양성 대조군인 acabose 보다 유의하게 강한 억제 효과가 있음을 보여주었다(p<0.05). 또한 STZ으로 유도된 당뇨병 마우스에서는 대조군의 높은 혈당과 달리 LPE 첨가군에서는 혈당이 유의하게 감소하였다(p<0.05). 따라서, 이들 결과는 LPE가 α-glucosidase와 α-amylase 억제 효과로 식후 고혈당을 감소시킬 수 있는 천연 항고혈당 식품으로 사용 가능성을 시사한다.

Abstract ▼ AI-Helper

This study was designed to investigate whether Loranthus parasiticus extract (LPE) could inhibit the activities of carbohydrate digestive enzymes and alleviate postprandial hyperglycemia in diabetic mice. Lyophilized L. parasiticus was extracted with 80% ethanol and concentrated. The inhibitory effects of LPE on carbohydrate digestive enzymes were evaluated by examining α-glucosidase and αamylase, and it was seen to inhibit the activities of both enzymes in a dose-dependent manner. More specifically, the IC50 values of LPE against α-glucosidase and α-amylase were 0.121±0.007 and 0.157±0.004 mg/ml, respectively, significantly lower than those of acarbose, showing that LPE has stronger inhibitory effects than the positive control. These results suggest that LPE strongly inhibits the activities of these digestive enzymes. Blood glucose levels in the control group of diabetic mice increased to 490.00±28.52 mg/dl and 474.60±25.30 mg/dl at 60 and 120 min after a meal, respectively. However, when LPE was added to starch, postprandial blood glucose levels were significantly reduced (463.0±23.73 and 418.5±24.50 mg/dl at 60 and 120 min, respectively; p<0.05). The area under the curve also significantly decreased following administration of LPE, with no cytotoxicity. These results therefore indicate that LPE could be used as an α-glucosidase and α-amylase inhibitor and delay carbohydrate digestion and, thus, glucose absorption after a meal.

주제어

표/그림 (7)

그림 Fig. 1. α-Glucosidase inhibitory effects of L. parasiticus extract (LPE).
그림 Fig. 2. α-Amylase inhibitory effects of L. parasiticus extract (LPE).
그림 Fig. 3. Cytotoxic effects of L. parasiticus extract (LPE) in 3T3-L1 cells. 3T3-L1 cells were treated with various concentrations (0.1, 0.2, 0.5, 1.0 and 2.0 mg/ml) of LPE for 20 hr, and cell viability was measured by MTT assay.
그림 Fig. 4. Blood glucose levels after the administration of L. parasiticus extract (LPE) in streptozotocin-induced diabetic mice.
표 Table 1. IC₅₀ values of the inhibitory effect of L. parasiticus extract (LPE) against α-glucosidase and α-amylase activities
그림 Fig. 5. Blood glucose levels after the administration of L. parasiticus extract (LPE) in normal mice.
표 Table 2. Areas under the curve (AUC) of postprandial glucose responses in normal and streptozotocin-induced diabetic mice

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제안 방법

This study investigated the inhibitory effects of the natural product, LPE, against α-amylase and α-glucosidase to uncover potential as a postprandial hyperglycemic inhibitor.

대상 데이터

Four-week-old male mice (ICR, Orient, Inc., Seoul, Korea) were individually housed in a temperature control room (25-30°C) with 45-55% relative humidity.

데이터처리

Differences were assessed with one-way ANOVA followed by Duncan's multi-range test (p<0.05).
Values with different letters (a-c) are significantly different at each time (p<0.05) as analyzed by Duncan’s multiple range test.
Values with different letters (a-d) are significantly different at p<0.05 as analyzed by Duncan’s multiple range test.
a-cValues with different alphabets are significantly different at p<0.05, as analyzed by Duncan's multiple-range test.

이론/모형

Blood glucose was measured using a glucometer (Roche Diagnostics GmbH, Mannheim, Germany). The areas under the curve (AUC) were calculated using the trapezoidal rule [17].

성능/효과

In conclusion, LPE inhibited α-glucosidase and α-amylase activities and resulted in a reduction in postprandial hyperglycemia.
In this study, LPE showed significantly higher inhibitory activities against both α-amylase and α-glucosidase than acarbose, the commercial inhibitor.
, DMSO used as the test solution). The measurements were performed in triplicate, and the IC₅₀value (i.e., the concentration of LPE that results in 50% inhibition of maximal activity) was determined.
Additionally, some triterpenes significantly decreased the hyperglycemia in diabetic rats by inhibiting small intestinal α-amylase, sucrase and α-glucosidase activity [15]. The results of this study showed that LPE could delay the digestion and absorption of dietary carbohydrates in the intestine, which could suppress the rise in blood glucose levels after meals in diabetic mice. The suppression effect on postprandial hyperglycemia of LPE was thought to be due to the active ingredients contained in LPE, such as sesquiterpene lactones, triterpenes and flavonoids.
Therefore, this study was conducted to determine whether L. parasiticus extract (LPE) inhibits α-amylase and α-glucosidase activities in vitro and suppresses postprandial hyperglycemia in diabetic mice in vivo.

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