식품위해성균, 피부사상균 및 식물성 병원균에 대한 산초유의 항균 활성 및 항산화 활성 Antimicrobial Activity against Food-hazardous Microorganisms, Dermatophytes, and Pytopathogens and Antioxidative Activity of Sancho Oil원문보기
Background: Although Sancho (Zanthoxylum schinifolium Siebold & Zucc) oil has traditionally been used for its antibiotics properties, there is currently a lack of scientific evidence regarding its biological activities. In this study, we investigated the antimicrobial and antioxidant activities of S...
Background: Although Sancho (Zanthoxylum schinifolium Siebold & Zucc) oil has traditionally been used for its antibiotics properties, there is currently a lack of scientific evidence regarding its biological activities. In this study, we investigated the antimicrobial and antioxidant activities of Sancho oil against food-hazardous microorganisms, phytopathogens, and dermatophytes. Methods and Results: We investiated the antimicrobial activity of Sancho oil against 11 food-hazardous microorganisms, nine phytopathogens, and six dermatophytes. The Sancho oil was found to show the strongest antibacterial activity against Shigella flexneri and Listeria spp. Sancho oil also showed high antifungal activity against plant pathogens, particularly Fusarium oxysporum, and showed antimicrobial activity against dermatophytes such as Trichophyton rubrum, Microsporum canis and Candida albicans. The antioxidant activity of Sancho oil was measured using the DPPH method, and was found to be stronger than that of unrefined oil. Moreover, this activity increased with increasing oil concentration. Conclusions: We found that Sancho oil showed differing antimicrobial activities against food-hazardous microorganisms, dermatophytes, and plant pathogens. The antimicrobial activity spectrum of Sancho oil was not broad and varied among microbial strains. On the basis of our findings, we consider that Sancho oil could be used an antibacterial material for food-borne S. flexneri and Listeria spp., a biopesticide for Fusarium spp., and a treatment for dermatophytes such as T. rubrum.
Background: Although Sancho (Zanthoxylum schinifolium Siebold & Zucc) oil has traditionally been used for its antibiotics properties, there is currently a lack of scientific evidence regarding its biological activities. In this study, we investigated the antimicrobial and antioxidant activities of Sancho oil against food-hazardous microorganisms, phytopathogens, and dermatophytes. Methods and Results: We investiated the antimicrobial activity of Sancho oil against 11 food-hazardous microorganisms, nine phytopathogens, and six dermatophytes. The Sancho oil was found to show the strongest antibacterial activity against Shigella flexneri and Listeria spp. Sancho oil also showed high antifungal activity against plant pathogens, particularly Fusarium oxysporum, and showed antimicrobial activity against dermatophytes such as Trichophyton rubrum, Microsporum canis and Candida albicans. The antioxidant activity of Sancho oil was measured using the DPPH method, and was found to be stronger than that of unrefined oil. Moreover, this activity increased with increasing oil concentration. Conclusions: We found that Sancho oil showed differing antimicrobial activities against food-hazardous microorganisms, dermatophytes, and plant pathogens. The antimicrobial activity spectrum of Sancho oil was not broad and varied among microbial strains. On the basis of our findings, we consider that Sancho oil could be used an antibacterial material for food-borne S. flexneri and Listeria spp., a biopesticide for Fusarium spp., and a treatment for dermatophytes such as T. rubrum.
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대상 데이터
The Sancho (Zanthoxylum schinifolium Siebold & Zucc) oil used for the antimicrobial test according to acidity was collected from Hadong J. Farm (Hadong, Korea) on November 25, 2012, and Sancho oil stored for 1 year was used high acidity Sancho oil (HA1), low acidity Sancho oil (LA1) was harvested in November 2013 and examined for its antibacterial activity.
The microorganisms used in this study were 11 foodhazardous microorganisms, 6 dermatophytes, and 9 plant pathogens (Table 1). Microbial strains were purchased from KCTC (Korean Collection for Type Cultures, Jeongup, Korea), KCCM (Korean Culture Center of Microorganisms, Seoul, Korea) and KACC (Korean Agricultural Culture Collection, Wanju, Korea).
데이터처리
All experiments were performed 3 times. Data are presented as means and standard deviation and analyzed by One-way ANOVA using the IBM SPSS statistical package (Ver. 24, IBM Co., Armonk, NY, USA). Means were compared at 5% significance level using Duncan’s Multiple Range Test (DMRT) comparison (p < 0.
이론/모형
Antimicrobial activity assay was determined by the disk agar plate diffusion method. Each test strain was incubated in the Tiyptic soy (TS) agar liquid medium for 24 hours, and the plate medium was prepared by coagulating 20 ㎖ of 1.
More methods have been tried to monitor the growth rate of fungi, and the measurement of the hyphae dry weight or increased colony diameter obtained in liquid culture. In this study, the antifungal activity of Sancho oil was assayed using the agar plate-dry weight method. The agar plate-dry weight method is considered a very good method as a tool for assessing mycelial growth of vegetable oil etc.
성능/효과
After incubation at 20℃ for 20 minutes, absorbance was measured at 517 ㎚. Electron donating ability (EDA, %) was measured by the difference of the absorbance of the sample added and blank sample as a percentage (%).
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