[국내논문]Effect of Melissa officinalis L. leaf extract on lipid accumulation by modulating specific adipogenic gene transcription factors in 3T3-L1 adipocytes원문보기
The objective of this study was to investigate the effects of a hypodermic injectable solution comprised of an LPM LB meso solution containing Melissa officinalis L. leaf extract (LPM) on the lipogenesis in the 3T3-L1 cells line. The lipid accumulation measured by oil red o staining in the 3T3-L1 ad...
The objective of this study was to investigate the effects of a hypodermic injectable solution comprised of an LPM LB meso solution containing Melissa officinalis L. leaf extract (LPM) on the lipogenesis in the 3T3-L1 cells line. The lipid accumulation measured by oil red o staining in the 3T3-L1 adipocytes treated with LPM, which was reduced in a dose dependent manner and showed 91.7 to 62.9% compared to control group. Its effectiveness with a 50% solution was significantly higher than the hydroxycitric acid (positive control) treatment without showing cell cytotoxicity. In a quantitative real-time PCR, it was demonstrated that the LPM treatment appeared to upregulate the mRNA expression of the adipogenesis-related genes, which included the peroxisome proliferator-activated receptor gamma (50% concentration) while down-regulating the CCAAT-enhancer binding protein alpha (50% concentration) and the sterol regulatory element-binding protein 1c (10, 25, and 50% concentrations). The results from the current study suggest that the LPM could be useful biomaterials that can inhibit obesity in the 3T3-L1 cells, which could possibly be by regulating the specific adipogenic gene transcription factors.
The objective of this study was to investigate the effects of a hypodermic injectable solution comprised of an LPM LB meso solution containing Melissa officinalis L. leaf extract (LPM) on the lipogenesis in the 3T3-L1 cells line. The lipid accumulation measured by oil red o staining in the 3T3-L1 adipocytes treated with LPM, which was reduced in a dose dependent manner and showed 91.7 to 62.9% compared to control group. Its effectiveness with a 50% solution was significantly higher than the hydroxycitric acid (positive control) treatment without showing cell cytotoxicity. In a quantitative real-time PCR, it was demonstrated that the LPM treatment appeared to upregulate the mRNA expression of the adipogenesis-related genes, which included the peroxisome proliferator-activated receptor gamma (50% concentration) while down-regulating the CCAAT-enhancer binding protein alpha (50% concentration) and the sterol regulatory element-binding protein 1c (10, 25, and 50% concentrations). The results from the current study suggest that the LPM could be useful biomaterials that can inhibit obesity in the 3T3-L1 cells, which could possibly be by regulating the specific adipogenic gene transcription factors.
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제안 방법
A 3T3-L1 cell (5×10 3 / well) was seeded in 96 well plates (SPL life Science, Pocheon, Republic of Korea), and it was then treated with various doses (1, 5, 10, 25, 50, and 100%) of the products.
All experiments were performed 4 times or 3 times, and the data is expressed as the mean ± standard deviation (SD) for the independent experiments.
In order to measure the cell viability using an MTT assay, different concentrations of the LPM LB meso solution comprising of the Melissa officinalis extraction (LPM) were treated in the 3T3-L1 adipocytes for 4 hours. The cell viability of the 3T3 cell ranged from 106.
The images were analyzed using an optical microscope (OLYMPUS model CKX41SF, Tokyo, Japan) at a 200× magnification and then spectrophotometric quantification was conducted at 500 nm.
Thus, the purpose of the current study was to evaluate the effect of the LPM LB meso solution containing Melissa officinalis L. leaf extract (LPM) on the cell cytotoxicity, the lipid accumulation, and the adipocytes gene modulation by various transcription factors, which included CEBPα, CCAAT-enhancer binding protein beta (CEBPβ), PPARγ, and SREBP1, by using the 3T3-preadipocyte cell model system.
데이터처리
All experiments were performed 4 times or 3 times, and the data is expressed as the mean ± standard deviation (SD) for the independent experiments. The mean difference between the groups was analyzed by one-way ANOVA using GraphPad Prism 3.0 (Graphpad, San Diego, CA, USA). A p value of less than 0.
성능/효과
The results from the current study concluded that 50% of the LPM solution seems to decrease the lipid accumulation by increasing the PPARγ expression and by also decreasing the CEBPα and the SREBP1 levels. The current study suggests the possible mechanisms of the LPM to inhibit adipogenesis, which include 1) adipogenic differentiation in the terminal stage could be inhibited by decreasing the CEBPα, 2) the BAT associated with weight loss could be induced by increasing the expression of the PPARγ, and 3) the enzyme activity on the lipid synthesis could be suppressed by down-regulating the gene expression of the SREBP1c.
According to this quantitative data obtained by quantifying the red colored portion, 10, 25, and 50% LPM solution contained lipid contents of 91.75±2.34, 86.85±0.89, and 62.87±3.25%, respectively, compared to the untreated control cells (Fig. 2B).
In conclusion, the current study investigated the effects of a hypodermic injectable solution compromising by Melissa officinalis L. leaf extract (LPM) on lipogenesis in the 3T3-L1 cells line. An LPM treatment that has up to a 50% solution reduced the lipid accumulation in the 3T3-L1 adipocytes.
The results from the current study concluded that 50% of the LPM solution seems to decrease the lipid accumulation by increasing the PPARγ expression and by also decreasing the CEBPα and the SREBP1 levels.
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