[논문]소건중탕 혼합단미연조엑스의 항염증 및 항위염 효과

김명진; 최혜민; 남원희; 김세진; 손수미; 김정옥

doi:10.15188/kjopp.2021.12.35.6.235

소건중탕 혼합단미연조엑스의 항염증 및 항위염 효과
Anti-inflammatory and Anti-gastritis Effect of Sogunjung-tang Mixed Soft Extract 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.35 no.6, 2021년, pp.235 - 241

김명진 (한국한의약진흥원) , 최혜민 (한국한의약진흥원) , 남원희 (한국한의약진흥원) , 김세진 (한국한의약진흥원) , 손수미 (한국한의약진흥원) , 김정옥 (한국한의약진흥원)

Abstract ▼ AI-Helper

Sogunjung-tang (SGJT) is traditional herbal prescriptions used to treat abdominal pain. In this study, we evaluated the effect on inflammation and gastritis through SGJT formulation development. SGJT composition herbal medicine was boiled in water at 95~100℃ for 3 hours and then concentrated. Sogunjung-tang mix soft extract (STM) was prepared using pharmaceutical excipients such as purified water, sodium benzoate, β-cyclodextrin and CMC-Na. Anti-inflammatory experiment was conducted using STM and lipopolysaccharide (LPS) in RAW 264.7 cells. Cell survival was measured by MTT method. Nitric oxide (NO) was measured using griess reagents, and pro inflammatory cytokines were measured by enzyme-linked immunosorbent assay and RT-PCR. Also, we verified STM validity in acute gastritis using the ICR mouse. STM was administered oral for three days. And 150 mM HCl in 60% ethanol was oral administered 0.5 mL one hour after the last drug administration. Mice were sacrificed 1 hour after 150 mM HCl in 60% ethanol administration. The gastric mucosa was visually observed. STM were not toxic in RAW 264.7 cells. Treatment of STM inhibited the production of NO and inflammatory cytokine at the protein and mRNA levels. Also, in the acute gastritis model with the mouse, the treatment of STM improved gastric mucosal bleeding and edema. In summary, it was confirmed that the treatment of STM exerts anti-inflammatory and anti-gastritis effects. Therefore, we suggest that STM may provide a preclinical evidence for the prevention and treatment of inflammatory and gastritis diseases.

주제어

표/그림 (7)

표 Table 1. Composition of Sogunjung-Tang
표 Table 2. Composition contents of Sogunjung-tang mix soft extract
그림 Fig. 1. Effect of Sogunjung-tang mix soft extract (STM) on cytotoxicity in RAW 264.7 cells. STM (50, 100 and 200 ㎍/mL) was incubated in RAW 264.7 cells for 24 hours. Cytotoxicity was measured by MTT assay. Similar results were obtained in three experiments.
그림 Fig. 2. Effects of Sogunjung-tang mix soft extract (STM) on NO Production in RAW 264.7 Cells. STM (50, 100 and 200 ㎍/mL) was pretreated for 1 hour and then incubated with LPS (500 ng/mL) for 24 hours. NO was measured. Similar results were obtained in three experiments. *P < 0.05 vs LPS treatment alone. "Dex" is "dexamethasone".
그림 Fig. 3. Effect of Sogunjung-tang mix soft extract (STM) on Pro-inflammatory Cytokine Production in RAW 264.7 Cells. STM (50, 100 and 200 ㎍/mL) was pretreated for 1 hour and then incubated with LPS (500 ng/mL) for 24 hours. Pro inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured by ELISA. Similar results were obtained in three experiments. *P < 0.05 vs LPS treatment alone. "Dex" is "dexamethasone".
그림 Fig. 4. Effects of Sogunjung-tang mix soft extract (STM) on the production of inflammatory cytokines at mRNA level in RAW 264.7 cells. STM was pre-treated in RAW 264.7 cells for 1 hour, and then incubated with LPS for 6 hours. The mRNA levels of IL-1β, IL-6, and TNF- α were determined by RT-PCR. Similar results were obtained in three experiments.
그림 Fig. 5. Effect of Sogunjung-tang mix soft extract (STM) on 150 mM HCl in 60% ethanol-induced gastritis. After pre treatment of STM (400 ㎎/㎏, oral) for 3 days, 0.5 mL of 150 mM HCl in 60% ethanol was injected 1 hour later. It was sacrificed 1 hour after 150 mM HCl in 60% ethanol oral administration. (A) Representative photographs of 150 mM HCl in 60% ethanol-induced gastritis. (B) Hematoxylin & eosin (H&E)-stained of histological sections of gastric mucosa in gastritis. (C) Inhibition ratio(%) of gastric mucosal damage in gastritis. Similar results were obtained in three experiments.

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