[논문]몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과

김숙진; 김건희

doi:10.7318/kjfc/2021.36.1.130

[국내논문] 몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과
Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells 원문보기

韓國食生活文化學會誌 = Journal of the Korean Society of Food Culture, v.36 no.1, 2021년, pp.130 - 142

김숙진 (덕성여자대학교 바이오헬스융합학과) , 김건희 (덕성여자대학교 바이오헬스융합학과)

Abstract ▼ AI-Helper

This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

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표/그림 (6)

표 Contents of total polyphenols and total flavonoids of 14 plants from Mongolia and Myanmar
표 Antioxidant activities of 14 plants from Mongolia and Myanmar
표 Pancreatic lipase inhibition activity and lipid peroxidation level of 14 plants from Mongolia and Myanmar
그림 Effect of 14 plants from Mongolia and Myanmar on cell viability in 3T3-L1 and HepG2 cell. Cells were incubated with 100, 200 and 400 μg/mL of extracts for 24 h. Cell viability of the treatment with extracts were determined in (A) 3T3-L1 and (B) HepG2 cell by MTT assay. Values are expressed as means ± SD of triplicate experiments. Significant differences between means were analyzed using ANOVA with Duncan’s multiple range test at p<0.05. DF, Dasiphora fruticosa Rydb.; CA, Chamaenerion angustifolium Scop.; GS, Glochidion sphaerogynum Kurz; MC, Mangifera caloneura Kurz; CG, Calamus arborescens Griff.; RA, Rosa acicularis Lindl.; SO, Sanguisorba officinalis L.; CL, Caragana arborescens Lam.; GK, Glochidion sphaerogynum Kurz.
그림 Effect of 14 plants from Mongolia and Myanmar on lipid accumulation in 3T3-L1 cell. (A) 3T3-L1 cells were stained with Oil Red O and observed under the microscope and (B) quantified lipid accumulation and (C) measured triglyceride content. Values are expressed as means ± SD of triplicate experiments. Significant differences between means were analyzed using ANOVA with Duncan’s multiple range test at p<0.05. DF, Dasiphora fruticosa Rydb.; CA, Chamaenerion angustifolium Scop.; GS, Glochidion sphaerogynum Kurz; MC, Mangifera caloneura Kurz; CG, Calamus arborescens Griff.
그림 Effect of 14 plants from Mongolia and Myanmar on lipid accumulation in HepG2 cell. (A) HepG2 cells were stained with Oil Red O and observed under the microscope and (B) quantified lipid accumulation and (C) measured triglyceride content. Values are expressed as means ± SD of triplicate experiments. Significant differences between means were analyzed using ANOVA with Duncan’s multiple range test at p<0.05. OA, Oleic acid; RA, Rosa acicularis Lindl.; SO, Sanguisorba officinalis L.; CA, Chamaenerion angustifolium Scop; CL, Caragana arborescens Lam.; GK, Glochidion sphaerogynum Kurz.

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