Lee, Jae Bum
(Department of Oriental Medical Food & nutrition, Semyung University)
,
Yang, Byung Wook
(School of Industrial Bio-Pharmaceutical Science, Semyung University)
,
Kim, Do Hyeong
(Department of Oriental Medical Food & nutrition, Semyung University)
,
Jin, Dezhong
(International Education College, Jiangxi University of Traditional Chinese Medicine)
,
Ko, Sung Kwon
(Department of Oriental Medical Food & nutrition, Semyung University)
The purpose of this study is to develop a new method of producing tienchi seng (notoginseng, Panax notoginseng) extracts featuring high concentrations of the ginsenoside Rg3, Rg5, and Rg6, special components of Korean red ginseng. The chemical transformation from ginseng saponin glycosides to prosap...
The purpose of this study is to develop a new method of producing tienchi seng (notoginseng, Panax notoginseng) extracts featuring high concentrations of the ginsenoside Rg3, Rg5, and Rg6, special components of Korean red ginseng. The chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by HPLC. Tienchi seng was heat-processed at 100℃ and the optimum conditions were identified. The highest concentrations of total saponin (29.723%) and the ginsenoside Rg3 (1.769%), Rg5 (5.979%), and Rg6 (13.473%) were produced at 48 hours. Also, when tienchi seng was subjected to the ultrasonic thermal fusion (100℃) process, the concentrations of total saponin (30.578%), ginsenoside Rg3 (2.392%), Rg5 (6.614%), and Rg6 (13.017%) were highest at 36 hours. On the other hand, the 2-hour heat-processed extract and 2-hour ultrasonic thermal fusion-processed extract did not contain ginsenoside Rg3, Rg5, and Rg6. The ultrasonic thermal fusion process had an extraction yield that was approximately 1.26 times greater than that of the heat process. These results indicate that the highly functional tienchi seng extracts created through the ultrasonic thermal fusion process are more industrially useful than those produced using the heat process.
The purpose of this study is to develop a new method of producing tienchi seng (notoginseng, Panax notoginseng) extracts featuring high concentrations of the ginsenoside Rg3, Rg5, and Rg6, special components of Korean red ginseng. The chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by HPLC. Tienchi seng was heat-processed at 100℃ and the optimum conditions were identified. The highest concentrations of total saponin (29.723%) and the ginsenoside Rg3 (1.769%), Rg5 (5.979%), and Rg6 (13.473%) were produced at 48 hours. Also, when tienchi seng was subjected to the ultrasonic thermal fusion (100℃) process, the concentrations of total saponin (30.578%), ginsenoside Rg3 (2.392%), Rg5 (6.614%), and Rg6 (13.017%) were highest at 36 hours. On the other hand, the 2-hour heat-processed extract and 2-hour ultrasonic thermal fusion-processed extract did not contain ginsenoside Rg3, Rg5, and Rg6. The ultrasonic thermal fusion process had an extraction yield that was approximately 1.26 times greater than that of the heat process. These results indicate that the highly functional tienchi seng extracts created through the ultrasonic thermal fusion process are more industrially useful than those produced using the heat process.
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제안 방법
The purpose of this study was to investigate the optimum conditions for production of high concentrations of the ginsenoside Rg3, Rg5, and Rg6 by comparing and analyzing the individual ginsenoside contents of the tienchi seng extract obtained through an ultrasonic thermal fusion process. The ginseng saponins that were analyzed included ginsenoside Rb1, Rc, Rd, Re, Rg1, Rg2, Rg3(s), Rg3(r), Rg5(e), Rg5(z), Rg6, Rh2, Rh4, Rk1, Rk3, F1, and F4; these were directly compared with the standard samples and confirmed through HPLC (Fig.
contents, and Rg5(z) and Rk1 were calculated from the sum of the two components. Tienchi seng was subjected to an ultrasonic thermal fusion process and heat process for 2, 4, 8, 12, 16, 20, 24, 36, 48, 60 and 72 hours, and the saponin content of each extract was calculated. When tienchi seng was subjected to ultrasonic thermal fusion, the crude saponin content was 60.
대상 데이터
Materials–The tienchi seng (notoginseng, Panax notoginseng) used in this study was 2kg of three-year-old roots purchased on May 18, 2018 in Changsha, Hunan, China. The tienchi seng specimens were kept in the Oriental medicine science laboratory at Semyung University (Fig.
The tienchi seng specimens were kept in the Oriental medicine science laboratory at Semyung University (Fig. 1).
The pure ginsenoside standards (99% purity) used in this experiment were purchased from Chromadex (SantaAna, CA, USA) and Koyeon (Jecheon, Chung-cheong-bukdo, Korea). The HPLC instrument used was a Waters 1525 binary HPLC system (Waters, Milford, MA, USA) with a Eurospher II 100-5 C18 column (250×3mm; Knauer, Berlin, Germany). The mobile phase was a mixture of acetonitrile (HPLC grade; Burdick&Jackson, 718 Cheo- yong-ro Nam-gu Ulsan, Korea) and distilled water (HPLC grade; Burdick&Jackson, 718 Cheoyong-ro Nam-gu Ulsan, Korea).
성능/효과
The 36-hour ultrasonic thermal fusion-processed extract showed the highest total saponin content (Table 3). The heat processed extract had a total saponin content of 29.665% for HPN-48, 27.917% for HPN-60, and 24.745% for HPN-36, and the 48-hour heat-processed extract showed the highest total saponin content (Table 2).
These prosapogenin components are known to have stronger physiological activity than the glycoside components because of their good cell affinity. The highest total content of these prosapogenin components was 27.594% (UHPN-36), which was obtained from 36 hours of ultrasonic thermal fusion, followed by UHPN-48 (23.649%) and UHPN-24 (19.389%). On the other hand, the content of prosapogenin in the heat-processed extract was 26.
In conclusion, the 2-hour ultrasonic thermal fusion and heat processes did not produce functional prosapogenin components, such as ginsenoside Rg3, Rg5 and Rg6, but after 4 hours, functional prosapogenin components were contained in the extracts. In addition, the 36-hour ultrasonic fusion extract contained the highest concentration of prosapogenin among all ultrasonic fusion extracts.
참고문헌 (43)
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