[논문]복분자 에탄올 추출물 및 분획물의 항산화 효과

강현; 이성규

doi:10.5010/jpb.2021.48.4.264

복분자 에탄올 추출물 및 분획물의 항산화 효과
Antioxidant Capacity of Ethanol Extracts and Fractions from Rubus coreanus Miq. 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.48 no.4, 2021년, pp.264 - 270

강현 (단국대학교 보건복지대학 임상병리학과) , 이성규 (단국대학교 보건복지대학 임상병리학과)

초록
AI-Helper

본 연구는 복분자 에탄올 추출물과 용매 분획물의 총 폴리페놀 함량 및 총 플라보노이드 함량과 라디칼 소거능과 환원력을 이용한 항산화 활성 측정하였다. 총 폴리페놀 함량은 70% 에탄올 추출물 238.46 mg GAE/g 으로 나타났으며, ethyl acetate 분획물 413.59 mg GAE/g 으로 다른 분획물보다 높은 함량을 나타내었다(p<0.05). 또한 총 플라보노이드 함량도 ethyl acetate 분획물에서 52.83 mg QE/g으로 높은 함량을 나타내었다(p<0.05). DPPH 라디칼 소거능과 ABTS 라디칼 소거능의 경우 ethyl acetate 분획물의 RC₅₀값이 각각 3.95와 6.25 ㎍/mL 으로 대조군인 ascorbic acid와 유사한 소거능을 나타내었다(p<0.05). FRAP에 의한 환원력 측정 결과에서도 ethyl acetate 분획물에서 높은 활성을 나타내었다(p<0.05). 이상의 결과 복분자 70% 에탄올 추출물과 용매 분획물 중 ethyl acetate 분획물이 다량의 폴리페놀과 플라보노이드를 함유하고 있으며, 항산화 효과가 높게 나타났다. 따라서 본 연구의 결과는 복분자를 기능성 신소재 개발 원료로 활용하는데 기초 자료가 될 것으로 기대된다.

Abstract ▼ AI-Helper

In the present study, the total polyphenol and flavonoid contents, radical scavenging ability, and reducing power of ethanol extracts and fractions from Rubus coreanus Miq. were assessed in order to determine the antioxidant activity. The total polyphenol contents in the 70% ethanol extract and ethyl acetate fraction were 238.46 mg gallic acid equivalents (GAE)/g and 413.59 mg GAE/g, respectively; these values were higher than those in the other fractions (p < 0.05). In addition, the total flavonoid content was as high as 52.83 mg quercetin equivalents (QE)/g in the ethyl acetate fraction (p < 0.05). With regard to DPPH and ABTS radical scavenging activities, the RC50 values of the ethyl acetate fraction were 3.95 and 6.25 ㎍/mL, respectively, indicating that the scavenging activity was similar to that of ascorbic acid (control) (p < 0.05). Moreover, in the FRAP assay performed to assess the reducing power, the ethyl acetate fraction showed high activity (p < 0.05). In summary, the present findings confirmed that the ethyl acetate fraction of the R. coreanus Miq. 70% ethanol extract had high polyphenol and flavonoid contents and excellent antioxidant properties. Therefore, it was confirmed that the ethyl acetate fraction of the mugwort 70% ethanol extract had high polyphenol and flavonoid content and excellent antioxidant effect.

주제어

표/그림 (5)

$Fig. 1. Schematic diagram of preparation of Rubus coreanus Miq. extract and fractions. (A) Ethanol extract preparation. (B) Fraction preparation$ 그림 Fig. 1. Schematic diagram of preparation of Rubus coreanus Miq. extract and fractions. (A) Ethanol extract preparation. (B) Fraction preparation
$Fig 2. Total polyphenol contents in ethanol extracts and fractions from Rubus coreanus Miq. Total polyphenol content, analyzed as gallic acid equivalents (GAE) mg/g of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig 2. Total polyphenol contents in ethanol extracts and fractions from Rubus coreanus Miq. Total polyphenol content, analyzed as gallic acid equivalents (GAE) mg/g of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test
$Fig. 3. Total flavonoid contents in ethanol extracts and fractions from Rubus coreanus Miq. Total flavonoid content, analyzed as quercetin equivalents (QE) mg/g of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig. 3. Total flavonoid contents in ethanol extracts and fractions from Rubus coreanus Miq. Total flavonoid content, analyzed as quercetin equivalents (QE) mg/g of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test
$Fig. 4. DPPH radical and ABTS+ radical scavenging activities of ethanol extracts and fractions from Rubus coreanus Miq. Amount required for 50% reduction of scavenging activity. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig. 4. DPPH radical and ABTS⁺ radical scavenging activities of ethanol extracts and fractions from Rubus coreanus Miq. Amount required for 50% reduction of scavenging activity. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test
$Fig. 5. Reducing power of ethanol extracts and fractions from Rubus coreanus Miq. FRAP value, analyzed as FeSO4 equivalents (FeSO4E) mM/mg of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test$ 그림 Fig. 5. Reducing power of ethanol extracts and fractions from Rubus coreanus Miq. FRAP value, analyzed as FeSO₄ equivalents (FeSO₄E) mM/mg of extract. Data are expressed as the means ± standard deviations (n = 3). Different superscripts in a column indicate significant differences at p < 0.05 using Duncan's multiple range test

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